| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 472,742 | (G)6→7 | coding (145/339 nt) | glnK → | nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 472,736 | 1 | . | G | 97.6% | 137.1 / ‑3.4 | 41 | coding (139/339 nt) | glnK | nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (21/19); total (22/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
ATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACC‑GG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGAAAAATTGGCGACG > NC_000913/472598‑472862 | aTGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTAcc > 1:1243042/1‑139 (MQ=255) gCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACC‑gg‑ggg > 1:68105/1‑139 (MQ=255) ggTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACC‑gg‑gggggc > 2:1171983/1‑137 (MQ=255) gTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACC‑gg‑gggggcg > 1:1256914/1‑136 (MQ=255) gTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACC‑gg‑gggggcg > 1:588957/1‑136 (MQ=255) gTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACC‑gg‑gggggcg > 1:532265/1‑136 (MQ=255) gTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACC‑gg‑gg > 1:1258874/1‑134 (MQ=255) gTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACC‑gg‑gg < 2:1258874/134‑1 (MQ=255) cGTGATAATCAAACCATTCAAGCTGGAGGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATa > 1:1050609/1‑139 (MQ=255) cGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATa > 1:760076/1‑139 (MQ=255) ttCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCGATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCCTCAGAAAGGGCATGACGAGCTGTACC‑GGCGGGCCGGAATACAGCGGCAGTTTACTg > 1:1375761/1‑139 (MQ=255) aaGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCa < 2:566526/139‑1 (MQ=255) gCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCaaa < 1:1171983/139‑1 (MQ=255) cGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAAt < 2:760076/139‑1 (MQ=255) gTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACC‑gg‑ggggg > 1:432073/1‑100 (MQ=255) gTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACC‑gg‑ggggg < 2:432073/101‑2 (MQ=255) cGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGa < 2:532265/139‑1 (MQ=255) cTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCtg < 2:1184051/139‑1 (MQ=255) tGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACC‑gg‑gg > 2:32699/1‑75 (MQ=255) tGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACC‑gg‑gg < 1:32699/75‑1 (MQ=255) gTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCa > 2:431248/1‑137 (MQ=255) gTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCa < 1:431248/137‑1 (MQ=255) gTATTCAGGGCCTGACCGACACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAAc > 1:816999/1‑139 (MQ=255) gggCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGa > 2:1300871/1‑139 (MQ=255) ggCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCaa < 1:239271/95‑1 (MQ=255) ggCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCaa > 2:239271/1‑95 (MQ=255) ggCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGaa > 1:645310/1‑139 (MQ=255) ggCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGaa > 2:7259/1‑139 (MQ=255) gCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAg > 1:395229/1‑139 (MQ=255) cTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTg > 1:98776/1‑139 (MQ=255) tGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGa > 2:628865/1‑139 (MQ=255) gACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGAt > 1:493705/1‑139 (MQ=255) gACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGAt > 1:747650/1‑139 (MQ=255) gTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAGGTGATCGat < 2:1256914/139‑1 (MQ=255) gTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGat < 2:98776/139‑1 (MQ=255) gTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGat < 2:1050609/139‑1 (MQ=255) gTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGat < 2:588957/139‑1 (MQ=255) cACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGatat < 2:68105/139‑1 (MQ=255) aCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGAtgt > 2:315973/1‑99 (MQ=255) aCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGAtgt < 1:315973/99‑1 (MQ=255) aCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGa < 2:67164/104‑1 (MQ=255) aCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGa > 1:67164/1‑104 (MQ=255) tGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAATGATCGATATCGTCAGTa < 2:645310/139‑1 (MQ=255) aaaGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAg < 2:816999/139‑1 (MQ=255) aaaGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAg < 1:1300871/139‑1 (MQ=255) aaaGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACC‑gg‑gg > 1:168226/1‑44 (MQ=255) aaaGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACC‑gg‑gg < 2:168226/44‑1 (MQ=255) aaGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAgg < 2:395229/139‑1 (MQ=255) aGGTTTCGGGCGTCAGAAAGGGCATGCAGAGCAGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAggc > 1:874982/1‑139 (MQ=255) cGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCCGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTa > 2:145439/1‑139 (MQ=255) tCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCa > 2:768618/1‑48 (MQ=255) tCAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCa < 1:768618/48‑1 (MQ=255) cAGAAAGGGCATGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGAAGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGa > 1:1338553/1‑139 (MQ=255) aTGCCGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGAAAAATTGGCg > 1:948677/1‑139 (MQ=255) ccGAGCTGTACCGGG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGAAAAATTGGCGACg > 2:344601/1‑139 (MQ=255) | ATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACC‑GG‑GGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGAAAAATTGGCGACG > NC_000913/472598‑472862 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |