| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,399,985 | (C)7→8 | coding (252/516 nt) | ogt ← | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,399,978 | 1 | . | C | 97.2% | 121.5 / ‑3.2 | 36 | coding (259/516 nt) | ogt | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (17/18); total (17/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01 | |||||||||||
GGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAA‑TGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCC > NC_000913/1399842‑1400111 | ggATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAA‑TGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑cc < 2:206579/139‑1 (MQ=255) aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAA‑TGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccg < 1:1029468/139‑3 (MQ=255) aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAA‑TGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccg > 1:116132/1‑137 (MQ=255) aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAA‑TGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccg < 2:821313/139‑3 (MQ=255) cGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAA‑TGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccgtag < 2:1281857/139‑6 (MQ=255) gTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAA‑TGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGc < 1:736316/139‑1 (MQ=255) tGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAA‑TGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGt > 1:409478/1‑139 (MQ=255) cGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAA‑TGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccg < 2:346319/129‑3 (MQ=255) cGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAA‑TGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccg > 1:346319/1‑127 (MQ=255) gCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAA‑TGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGa < 1:579001/139‑1 (MQ=255) gCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAA‑TGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGa < 2:1385383/139‑1 (MQ=255) aaCGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAA‑TGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGt < 2:1255710/139‑1 (MQ=255) cgcgccgcgcCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAA‑TGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCaata > 2:818890/1‑139 (MQ=255) cgggccggaaCGGCCCAATTGCTAAGCCATTTGG‑CGTAATTGCATTCCCTGCCGCAAGGGGTTAGGGCGTATTTTTTTCCAGACTTCGCGCTGAATTGCCGCC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCt < 1:959767/130‑1 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAA‑TGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCa > 2:180056/1‑139 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAA‑TGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑cc < 1:1009892/91‑1 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAA‑TGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑cc > 2:1009892/1‑91 (MQ=255) cAGCCAGTTGGCCGTAA‑TGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCAc > 2:641848/1‑139 (MQ=255) tACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGcc < 1:180056/139‑1 (MQ=255) gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTGAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGAt < 2:834706/139‑1 (MQ=255) gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGAt > 1:534804/1‑139 (MQ=255) gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccg > 1:471464/1‑59 (MQ=255) gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccg < 2:471464/61‑3 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGAtt > 1:1359763/1‑139 (MQ=255) ccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATtg > 2:1366219/1‑139 (MQ=255) cAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATtggtg > 2:318962/1‑139 (MQ=255) cAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATtggtg > 2:1429106/1‑139 (MQ=255) aGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATtggtgg < 1:318962/139‑1 (MQ=255) ggggATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGc > 1:538349/1‑68 (MQ=255) ggggATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGc < 2:538349/68‑1 (MQ=255) tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑cccc > 2:870532/1‑41 (MQ=255) tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑cccc < 1:870532/41‑1 (MQ=255) cGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgcgc < 1:1429106/139‑1 (MQ=255) aGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGc < 1:1162898/68‑1 (MQ=255) aGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGc > 2:1162898/1‑68 (MQ=255) aGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCa > 2:1019142/1‑139 (MQ=255) tgtTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATa > 2:198796/1‑139 (MQ=255) tttCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATg > 2:1440156/1‑128 (MQ=255) tttCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATg < 1:1440156/128‑1 (MQ=255) tCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTggg > 2:211584/1‑44 (MQ=255) tCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTggg < 1:211584/44‑1 (MQ=255) aGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCcttctt < 1:641848/139‑1 (MQ=255) ttCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATGGCCTTCTTTGCg > 2:1404218/1‑139 (MQ=255) cgcgCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaa < 1:837571/92‑1 (MQ=255) cgcgCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaa > 2:837571/1‑92 (MQ=255) cTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATGGCCTTCTTTGCGATAATg < 1:1404218/139‑1 (MQ=255) ggCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTcc < 2:409478/139‑1 (MQ=255) ggCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTcc < 1:1366219/139‑1 (MQ=255) ggCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTcc < 1:1176539/139‑1 (MQ=255) | GGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAA‑TGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCC > NC_000913/1399842‑1400111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |