| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,700,201 | (T)5→6 | coding (847/1593 nt) | malX → | maltose and glucose‑specific PTS enzyme IIB component and IIC component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,700,196 | 1 | . | T | 97.4% | 115.7 / ‑3.5 | 38 | coding (842/1593 nt) | malX | maltose and glucose‑specific PTS enzyme IIB component and IIC component |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (17/20); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
TGGTCTGCATCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑G‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAG > NC_000913/1700065‑1700321 | tGGTCTGCATCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑G‑ttttttc > 2:217424/1‑137 (MQ=255) cTGCATCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGaa < 2:1316827/139‑1 (MQ=255) cATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCttt > 2:230591/1‑139 (MQ=255) ttAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCg < 1:700093/139‑1 (MQ=255) aTTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCaa < 2:185/139‑1 (MQ=255) gCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCg > 1:474849/1‑139 (MQ=255) ggcggcACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGttt > 1:434555/1‑139 (MQ=255) cGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTcggcg > 2:1429931/1‑139 (MQ=255) cGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGc < 1:1154226/139‑1 (MQ=255) cGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGc > 1:39930/1‑139 (MQ=255) gTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGc < 2:1155091/137‑1 (MQ=255) gTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGc > 1:1155091/1‑137 (MQ=255) tCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTcggcg > 2:639147/1‑123 (MQ=255) tCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTcggcg < 1:639147/123‑1 (MQ=255) aaCCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCg > 1:1213813/1‑92 (MQ=255) aaCCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCg < 2:1213813/92‑1 (MQ=255) aaCCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTcggcg < 2:749989/120‑1 (MQ=255) aaCCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTcggcg > 1:749989/1‑120 (MQ=255) aaCCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGtt < 1:1429931/139‑1 (MQ=255) aaCCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGt < 2:970648/138‑1 (MQ=255) aaCCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTAAGTTGCCCGACCACTCACGAT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGt > 1:970648/1‑138 (MQ=255) ccGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAg < 1:217424/139‑1 (MQ=255) ccGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAg < 2:474849/139‑1 (MQ=255) aaTTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTcg > 2:1304928/1‑82 (MQ=255) aaTTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTcg < 1:1304928/82‑1 (MQ=255) aTTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCa > 2:1174581/1‑139 (MQ=255) ttGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCAt < 2:1172981/139‑1 (MQ=255) ttGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGc > 1:331661/1‑51 (MQ=255) ttGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGc < 2:331661/51‑1 (MQ=255) cccGACCATTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGt < 1:739115/93‑1 (MQ=255) cccGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTGCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGtt < 1:111214/94‑1 (MQ=255) cccGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTGCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGtt > 2:111214/1‑94 (MQ=255) cccGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGcc < 1:207332/82‑1 (MQ=255) cccGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGcc > 2:207332/1‑82 (MQ=255) cccGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGt > 2:739115/1‑93 (MQ=255) cccGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTaa > 2:1385428/1‑139 (MQ=255) cGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAg < 2:39930/139‑1 (MQ=255) ccACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCaa > 2:591493/1‑45 (MQ=255) ccACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCaa < 1:591493/45‑1 (MQ=255) | TGGTCTGCATCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑G‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAG > NC_000913/1700065‑1700321 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |