| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 197,757 | (G)6→7 | coding (1212/1353 nt) | rseP → | inner membrane zinc RIP metalloprotease, RpoE activator, by degrading RseA, cleaved signal peptide endoprotease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 197,751 | 1 | . | G | 96.0% | 80.6 / ‑3.0 | 25 | coding (1206/1353 nt) | rseP | inner membrane zinc RIP metalloprotease, RpoE activator, by degrading RseA, cleaved signal peptide endoprotease |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (11/13); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.69e-01 | |||||||||||
CCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGAC‑GGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATG > NC_000913/197616‑197881 | ccTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGAC‑ggg < 2:1420828/139‑1 (MQ=255) gTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGAC‑ggggg‑ggc < 2:238373/139‑3 (MQ=255) gTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGAC‑ggggg‑ggc < 1:764620/139‑3 (MQ=255) gTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCATTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCAGTACTTGAC‑ggggg‑ggc < 1:841245/139‑3 (MQ=255) gggCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGAC‑ggggg‑ggcat < 1:1384580/139‑5 (MQ=255) ggCCGATCTCTATCACCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGAC‑ggggg‑gggc < 2:117252/137‑4 (MQ=255) ggCCGATCTCTATCACCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGAC‑ggggg‑gggc > 1:117252/1‑134 (MQ=255) ggggCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGaa < 2:1403752/139‑1 (MQ=255) cTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGa > 2:1386912/1‑139 (MQ=255) gACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAggg < 2:965330/139‑1 (MQ=255) ttgttTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGc < 2:1475319/139‑1 (MQ=255) tttATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGAC‑ggggg‑ggc < 1:1060387/85‑3 (MQ=255) tttATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGAC‑ggggg‑ggc > 2:1060387/1‑83 (MQ=255) cTGCCGTTTCTTGCGCTTATTAGCGTGAATTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGAC‑GGGGGTGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGAc > 2:982729/1‑139 (MQ=255) gCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACtt < 1:1386912/139‑1 (MQ=255) gCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACtt > 2:1276765/1‑139 (MQ=255) gCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACtt > 1:727777/1‑139 (MQ=255) gCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACtt > 1:953974/1‑139 (MQ=255) gCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGAC‑ggggg‑ggc < 1:181976/75‑3 (MQ=255) gCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGAC‑ggggg‑ggc > 2:181976/1‑73 (MQ=255) ccGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACttt > 2:1021642/1‑139 (MQ=255) aCTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTctgc > 2:92525/1‑139 (MQ=255) cTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTctgct < 2:1195305/139‑1 (MQ=255) aGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGt < 1:1118826/139‑1 (MQ=255) ggATAATTAACCTGTTTCCGTTGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGc > 1:779804/1‑139 (MQ=255) ggATAATTAACCTGTTTCCGTTGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGc < 1:240178/139‑1 (MQ=255) gATAATTAACCTGTTTCCGTTGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCt < 2:727777/139‑1 (MQ=255) ttAACCTGTTTCCGTTGCCCGTACTTGAC‑ggggg‑ggc < 1:652681/37‑3 (MQ=255) ttAACCTGTTTCCGTTGCCCGTACTTGAC‑ggggg‑ggc > 2:652681/1‑35 (MQ=255) tAACCTGTTTCTGTTGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAAt < 2:953974/139‑1 (MQ=255) ccTGTTTCCGTTGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGAt > 2:1072710/1‑52 (MQ=255) ccTGTTTCCGTTGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGAt < 1:1072710/52‑1 (MQ=255) tCCGTTGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGc > 1:103695/1‑139 (MQ=255) cGTTGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCAc < 2:103695/139‑1 (MQ=255) ttGCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGAcc > 1:993687/1‑63 (MQ=255) ttGCCCGTACGTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGAcc < 2:993687/63‑1 (MQ=255) gCCCGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACtttt < 1:1230089/139‑1 (MQ=255) ccGTACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAGGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCa < 1:1025020/139‑1 (MQ=255) tACTTGACGGGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATg > 2:238774/1‑139 (MQ=255) | CCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTGAACTTAGGGATAATTAACCTGTTTCCGTTGCCCGTACTTGAC‑GGGGG‑GCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATG > NC_000913/197616‑197881 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |