| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,709,561 | (C)6→7 | coding (915/1692 nt) | eptB ← | KDO phosphoethanolamine transferase, Ca(2+)‑inducible |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,709,555 | 1 | . | C | 97.5% | 134.3 / ‑3.3 | 40 | coding (921/1692 nt) | eptB | KDO phosphoethanolamine transferase, Ca(2+)‑inducible |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (15/24); total (16/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01 | |||||||||||
GTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACC > NC_000913/3709423‑3709686 | gttgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc < 1:1385399/139‑1 (MQ=255) ttgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccc < 1:822139/139‑2 (MQ=255) tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccctga > 1:1221055/1‑135 (MQ=255) tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccctga > 2:215444/1‑135 (MQ=255) tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cc < 2:336091/131‑1 (MQ=255) tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cc > 1:336091/1‑131 (MQ=255) ccATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTa < 2:715631/139‑1 (MQ=255) ccATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTa < 1:1399847/139‑1 (MQ=255) ccATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTa < 2:1295061/139‑1 (MQ=255) tCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACaa < 2:1083793/139‑1 (MQ=255) tCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACaa < 2:1221055/139‑1 (MQ=255) tgtTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACg > 2:619216/1‑131 (MQ=255) tgtTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACg < 1:619216/131‑1 (MQ=255) aaCCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGt < 2:634407/139‑1 (MQ=255) acaTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTgaga > 2:993964/1‑139 (MQ=255) caTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc < 2:324309/109‑1 (MQ=255) caTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc > 1:324309/1‑109 (MQ=255) cGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCcgcg < 1:670095/98‑1 (MQ=255) cGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCcgcg > 2:670095/1‑98 (MQ=255) ctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc < 1:902984/139‑1 (MQ=255) gTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGt > 2:305951/1‑121 (MQ=255) gTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGt < 1:305951/121‑1 (MQ=255) aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACg < 2:200823/88‑1 (MQ=255) aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACg > 1:200823/1‑88 (MQ=255) aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcac > 1:800054/1‑129 (MQ=255) aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcac < 2:800054/129‑1 (MQ=255) aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAg > 1:1139031/1‑134 (MQ=255) aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAg < 2:1139031/134‑1 (MQ=255) aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc < 1:10973/75‑1 (MQ=255) aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc > 2:10973/1‑75 (MQ=255) aTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAcg < 1:993964/139‑1 (MQ=255) aaCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACgccgcc < 2:813816/139‑1 (MQ=255) aCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCa > 1:1175528/1‑139 (MQ=255) cTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccc > 1:989206/1‑64 (MQ=255) cTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccc < 2:989206/65‑2 (MQ=255) ttCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACg < 1:398313/69‑1 (MQ=255) ttCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACg > 2:398313/1‑69 (MQ=255) tCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAttt > 1:621893/1‑138 (MQ=255) tCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAttt < 2:621893/138‑1 (MQ=255) cAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c < 1:868098/56‑1 (MQ=255) cAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c > 2:868098/1‑56 (MQ=255) cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGt > 1:354911/1‑89 (MQ=255) cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGt < 2:354911/89‑1 (MQ=255) aaaaTGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGcc > 1:755853/1‑139 (MQ=255) ctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTaa < 2:34786/93‑1 (MQ=255) ctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTaa > 1:34786/1‑93 (MQ=255) ttAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTAt > 1:1345886/1‑139 (MQ=255) ttAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc < 1:306876/35‑1 (MQ=255) ttAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc > 2:306876/1‑35 (MQ=255) cgcTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCg > 2:603678/1‑122 (MQ=255) cgcTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCg < 1:603678/122‑1 (MQ=255) cgcTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTa < 1:133550/133‑1 (MQ=255) cgcTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTa > 2:133550/1‑133 (MQ=255) cacTGCGGATTATCTTCCGCG‑CCCCCCTTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa > 1:335810/3‑139 (MQ=255) gATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGa < 1:136212/139‑1 (MQ=255) aTCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAAt < 2:148872/139‑1 (MQ=255) cTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATAc < 2:1345886/139‑1 (MQ=255) ttCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATAcc < 1:215444/139‑1 (MQ=255) | GTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACC > NC_000913/3709423‑3709686 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |