| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 380,022 | (G)10→11 | intergenic (‑141/+47) | frmR ← / ← yaiO | regulator protein that represses frmRAB operon/outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 380,012 | 1 | . | G | 75.6% | 114.1 / 32.6 | 45 | intergenic (‑131/+57) | frmR/yaiO | regulator protein that represses frmRAB operon/outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (5/6); new base G (23/11); total (28/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑A‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAC > NC_000913/379874‑380135 | cTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGa‑a‑‑a < 1:411940/139‑1 (MQ=255) atcatcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑A‑‑gggggggggggctaaggg > 2:100696/1‑131 (MQ=255) cTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATGGGCAgg > 2:1476116/1‑139 (MQ=255) tgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAgg < 1:646666/139‑1 (MQ=255) ggggggATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCGGGGAAGGCCGCCCGGGAAAggg > 2:929254/1‑137 (MQ=255) gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AGGGGGGGGGGGGCTATTGGGCAGGGAAGGCCGCCCCGGATAgc > 2:1254060/1‑139 (MQ=255) gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATc > 2:773289/1‑113 (MQ=255) gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATc < 1:773289/113‑1 (MQ=255) gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg > 2:330223/1‑139 (MQ=255) gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg > 2:214175/1‑139 (MQ=255) gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg > 2:389949/1‑139 (MQ=255) gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg > 2:1393063/1‑139 (MQ=255) tATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑A‑‑‑GGGGGGGGGCTAATCGGCAGGGAAgg < 1:1183908/117‑1 (MQ=255) tATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑A‑‑‑GGGGGGGGGCTAATCGGCAGGGAAgg > 2:1183908/1‑117 (MQ=255) tGCAAACCCCCCTATAGTATAAAACACTGGAGAAAAAAATTTATCCGGTGAAGGTGGTCGGAAAAAAAAGAGGA‑AGGG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGa < 1:1254060/139‑1 (MQ=255) cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑A‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAttt > 2:15344/1‑139 (MQ=255) ataGTATATAACACTGGAAAATAAAATTTATTCGGTGAATGTGGTCGGAAAAAAAAGGGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGa < 2:1262807/139‑1 (MQ=255) tatataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACggg > 1:1466597/1‑139 (MQ=255) tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGc > 1:332180/1‑139 (MQ=255) tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt > 1:809253/1‑139 (MQ=255) tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑A‑‑ggggggggggg > 1:1347479/1‑67 (MQ=255) tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑A‑‑ggggggggggg < 2:1347479/68‑2 (MQ=255) aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑A‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCaa > 2:1212556/1‑139 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAg > 1:547357/1‑139 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGAAAGCGGGGGGAAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGAAAg > 1:1262807/1‑139 (MQ=255) cTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTATTGGGCGGGGAAGCCCCCCCGGGTAAGGGGGGGGAGGAAGGATTAAAAATTTCCGGGGCAGAGGGGcggaaggtt > 1:1191683/1‑131 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATTGGGAGGGAAGGGCGCCCCGGATAGCGGGCGGGAGAAGGAATCAAAATTTCCAGGGCAGGAGGGCTGCAAGttt > 2:227514/1‑138 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAgg > 1:572097/1‑77 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg > 2:104416/1‑139 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCACCGGATAGCGGGCGGCAGAAGGAATCAGATTTTCCAGGTCAGACGGGCTGCAAGTTg > 1:317326/1‑139 (MQ=255) tGGAGAATAAAATTTATCCGGGGAATGTGGGCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAgg < 2:572097/77‑1 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa > 1:230060/1‑139 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa > 1:3596/1‑139 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGAAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa > 2:127076/1‑139 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑A‑‑GGGGGGGGGGCAAATCGGTAGGGAAGGCCGCCCCGGATAGCCGGCGGCAGAAGGAAACAGAATTTCGAGGTTAGACGGGGTGCAAGTTGCAg > 2:962826/1‑139 (MQ=255) ggaAAAAAATTTTTCCGGGGAATGGGGTGGGAAAAAAAAAAGGA‑A‑‑A‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGTCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGa < 2:18984/138‑1 (MQ=255) gaAAAAAATTTTTCCGGGGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGa < 2:1191683/139‑1 (MQ=255) cccGATGAATGTGGGCGGAAAAACACGGAGA‑A‑‑A‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCATAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACTGTTAAAatcat < 1:568467/138‑1 (MQ=255) ccGGTGAATGTGGTCGGAAAAAAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatcat < 2:547357/139‑1 (MQ=255) tGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAgg > 1:337707/1‑56 (MQ=255) tGAAGGTGGTCGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAgg < 2:337707/56‑1 (MQ=255) ggaaaggggTCGGAAAAAAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGt < 1:1393063/132‑1 (MQ=255) gggggggggAAAACAAAGGGGA‑AG‑G‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTggg < 1:227514/132‑1 (MQ=255) ggTCGGAAAACAAAGAGGAGA‑‑G‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgtg < 1:330223/139‑1 (MQ=255) gTCGTAAAAAAAAAAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgtgt < 1:1476116/139‑1 (MQ=255) tCGGAAAACAAAGAGGA‑A‑‑A‑‑GGGGGGGGGGCCAATCGGCAGGGAAAGCCGCCCCGGAAAGGGGGGGGCAGAAGGGATCAGAATTTTCAGGTCAGAAGGGCCGGAAGTTGCCGGCCGGTAAAATCATTGGTTTGGGTGGCg > 2:1436808/1‑139 (MQ=255) cGGAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCg > 2:1161306/1‑139 (MQ=255) ggAAAACAAAGAGGA‑C‑‑A‑‑GGTGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCACAAGTTCCAGGTCAGCCGGGCTGCAACTTGCACACCGTTAAAATCATCGGTTGGGCTGTCGTa < 1:962826/139‑1 (MQ=255) gAAAACAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTa < 1:1161306/139‑1 (MQ=255) aaaaCAAAGAGGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAc < 1:559087/139‑1 (MQ=255) gggggA‑A‑‑G‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc < 1:264226/88‑1 (MQ=255) gagGA‑A‑‑AG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGACTTTCCAGGTCAGACGGGCTGc > 2:264226/1‑91 (MQ=255) | CTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑A‑‑A‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAC > NC_000913/379874‑380135 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |