Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,153,047	A→G	D370G (GAT→GGT)	fabF →	3‑oxoacyl‑[acyl‑carrier‑protein] synthase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,153,047	0	A	G	100.0%	225.7 / NA	64	D370G (GAT→GGT)	fabF	3‑oxoacyl‑[acyl‑carrier‑protein] synthase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/29);  total (35/29)

CAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAG  >  NC_000913/1152918‑1153174                                                                                                                                   |                                                                                                                                cAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGa                                                                                                                        >  2:1105963/1‑139 (MQ=255)            ttGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCg                                                                                                             <  2:1273295/139‑1 (MQ=255)              ggTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGAt                                                                                                           <  1:150501/139‑1 (MQ=255)                    cTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGCAGAATCTTTCTTCCCCCTCCCGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCCACCTGGGACACCCCGAAGGAAGGtgcggttttgat                                                                                                      >  1:516798/1‑128 (MQ=255)                           aaaTCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTAc                                                                                              <  1:1323273/139‑1 (MQ=255)                                 aTGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACg                                                                                        >  2:1348696/1‑139 (MQ=255)                                        gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTCCTCCATCCTGGCGCAGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGcgcg                                                                                 >  1:968613/1‑139 (MQ=255)                                        gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCCGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGcgcg                                                                                 >  2:792793/1‑139 (MQ=255)                                        gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTTCGATCTGGATTTCGCACCGCACGAAGcgcg                                                                                 >  2:55341/1‑139 (MQ=255)                                        gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTAc                                                                                              >  2:765417/1‑126 (MQ=255)                                        gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTAc                                                                                              <  1:765417/126‑1 (MQ=255)                                        gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAg                                                                                     <  2:1063815/135‑1 (MQ=255)                                        gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGcgcg                                                                                 >  2:1209093/1‑139 (MQ=255)                                        gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGcgcg                                                                                 >  2:772592/1‑139 (MQ=255)                                        gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGcgcg                                                                                 >  2:443459/1‑139 (MQ=255)                                        gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCCCCAACCATCCACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAg                                                                                     >  1:1063815/1‑135 (MQ=255)                                                ttAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTa                                                                         >  2:555062/1‑139 (MQ=255)                                                   ggTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCg                                                                      <  2:516798/139‑1 (MQ=255)                                                       cggcggGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaat                                                                  <  1:443459/139‑1 (MQ=255)                                                       cggcggGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaat                                                                  <  1:555062/139‑1 (MQ=255)                                                       cggcggGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaat                                                                  >  2:722547/1‑139 (MQ=255)                                                       cggcggGTGCAATAGGATCCATCTACTCCCTCCTGGCGCTGCGCGAACAGGCTGTTCCGCCAACCAACAACCT‑GGGTCACCCGGATGAAGGTTGCGGTCTGGGTTTTGTACCGCACGAAGCGCGTCATGTTAACggaat                                                                  >  1:600340/1‑139 (MQ=255)                                                               gCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAAc                                                                                                                             <  1:893684/72‑1 (MQ=255)                                                               gCAGTAGAATCTATATACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAAc                                                                                                                             >  2:893684/1‑72 (MQ=255)                                                                cAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATacac                                                         <  1:1209093/139‑1 (MQ=255)                                                                 aGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACAct                                                        <  1:722547/139‑1 (MQ=255)                                                                     gAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCtgt                                                    <  1:772592/139‑1 (MQ=255)                                                                        tctatctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGt                                                                                <  1:1163528/108‑1 (MQ=255)                                                                        tctatctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGt                                                                                >  2:1163528/1‑108 (MQ=255)                                                                        tctatctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCg                                                                      >  2:920146/1‑118 (MQ=255)                                                                        tctatctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCg                                                                      <  1:920146/118‑1 (MQ=255)                                                                        tctatctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTa                                                 >  1:584684/1‑139 (MQ=255)                                                                          tatctaCTCCATCCGGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCTTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAAc                                               >  2:729783/1‑139 (MQ=255)                                                                            tctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTc                                             >  2:514224/1‑139 (MQ=255)                                                                                cTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATaca                                                          <  2:424870/122‑1 (MQ=255)                                                                                cTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATaca                                                          >  1:424870/1‑122 (MQ=255)                                                                                cTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATac                                                           <  1:225946/121‑1 (MQ=255)                                                                                cTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATac                                                           >  2:225946/1‑121 (MQ=255)                                                                                cTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttc                                         >  1:1065764/1‑139 (MQ=255)                                                                                cTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttc                                         >  1:1265338/1‑139 (MQ=255)                                                                                 tCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACAct                                                        <  2:1182117/123‑1 (MQ=255)                                                                                 tCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACAct                                                        >  1:1182117/1‑123 (MQ=255)                                                                                   cATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttcggc                                      <  2:1065764/139‑1 (MQ=255)                                                                                      ccTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttcggcttc                                   >  2:553176/1‑139 (MQ=255)                                                                                       cTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttcggcttcg                                  <  1:514224/139‑1 (MQ=255)                                                                                         ggCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGt                                <  1:729783/139‑1 (MQ=255)                                                                                          gcgcTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAAcc                                                                                                                            <  1:566562/46‑1 (MQ=255)                                                                                          gcgcTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAAcc                                                                                                                            >  2:566562/1‑46 (MQ=255)                                                                                          gcgcTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTTCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaat                                                                  <  1:1126841/104‑1 (MQ=255)                                                                                          gcgcTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaat                                                                  >  2:1126841/1‑104 (MQ=255)                                                                                          gcgcTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCgga                                                                    <  1:921847/102‑1 (MQ=255)                                                                                          gcgcTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCgga                                                                    >  2:921847/1‑102 (MQ=255)                                                                                                cgcgATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttcggc                                      >  2:156536/1‑126 (MQ=255)                                                                                                cgcgATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttcggc                                      <  1:156536/126‑1 (MQ=255)                                                                                                cgcgATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTa                         >  1:583369/1‑139 (MQ=255)                                                                                                   gATCAGGCTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATg                      <  2:1265338/139‑1 (MQ=255)                                                                                                          cTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaatgga                                                               >  1:392122/1‑91 (MQ=255)                                                                                                          cTGTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaatgga                                                               <  2:392122/91‑1 (MQ=255)                                                                                                            gTTCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGa             <  1:553176/139‑1 (MQ=255)                                                                                                             ttCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGtt                                                                                                                <  2:696459/39‑1 (MQ=255)                                                                                                             ttCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGtt                                                                                                                >  1:696459/1‑39 (MQ=255)                                                                                                             ttCCGCCAACCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGAt            >  2:1342480/1‑139 (MQ=255)                                                                                                                    aaCCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTaaa     <  1:1348696/139‑1 (MQ=255)                                                                                                                     aCCATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTaaaa    >  2:780582/1‑139 (MQ=255)                                                                                                                       cATCAACCT‑GGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAg  >  1:123393/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                CAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCT‑GGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAG  >  NC_000913/1152918‑1153174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑