Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	152,036	C→T	V66I (GTA→ATA)	yadL ←	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	152,036	0	C	T	100.0%	152.4 / NA	47	V66I (GTA→ATA)	yadL	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/28);  total (19/28)

CAGTTCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGA  >  NC_000913/151907‑152156                                                                                                                                  |                                                                                                                          cAGTTCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCaaa                                                                                                                  <  1:1453747/139‑1 (MQ=255)    ttCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATc                                                                                                               >  1:1477145/1‑139 (MQ=255)      cccTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATcaa                                                                                                             <  2:554087/139‑1 (MQ=255)              tgtgGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGaa                                                                                                    <  1:230498/139‑1 (MQ=255)                 ggCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATC‑ACAACAAGAAATg                                                                                                 >  2:1031572/1‑139 (MQ=255)                  gCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGt                                                                                                >  1:310012/1‑139 (MQ=255)                   ctctcgtGGTTTATTTTATTTGCGCTTTTTTAGTGTGTATACCCGAACAGTTTATGAATTTGAGTTTTAGTGTTTTTTGTTTGGTTGGGGCATAGATTTGAAAAATATATATATCACCAAAATCAACAAC‑AGAAATGTc                                                                                               <  1:1031572/133‑1 (MQ=255)                   cTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTc                                                                                               <  1:1064950/139‑1 (MQ=255)                       cTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGa                                                                                           >  2:434435/1‑139 (MQ=255)                          ggTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCa                                                                                        <  1:576964/139‑1 (MQ=255)                                  ttATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCAtt                                                                                <  2:572288/139‑1 (MQ=255)                                                 tATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGt                                                                 <  2:1109308/139‑1 (MQ=255)                                                 tATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGt                                                                 <  1:876200/139‑1 (MQ=255)                                                  aTTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTa                                                                <  2:162624/139‑1 (MQ=255)                                                        gATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTc                                                                                               <  2:1126427/102‑1 (MQ=255)                                                        gATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTc                                                                                               >  1:1126427/1‑102 (MQ=255)                                                        gATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTa                                                          >  1:1061362/1‑139 (MQ=255)                                                          tACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTAcc                                                        <  1:434435/139‑1 (MQ=255)                                                          tACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTAcc                                                        <  2:568359/139‑1 (MQ=255)                                                           aCCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTAccc                                                       <  2:1061362/139‑1 (MQ=255)                                                                  caGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTa                                                <  2:671351/139‑1 (MQ=255)                                                                  caGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGACTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCagag                                                                                     >  1:633098/1‑102 (MQ=255)                                                                  caGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGACTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCagag                                                                                     <  2:633098/102‑1 (MQ=255)                                                                     tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGaa                                                                                                    <  1:38175/84‑1 (MQ=255)                                                                     tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGaa                                                                                                    >  2:38175/1‑84 (MQ=255)                                                                     tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTa                                                >  1:80292/1‑136 (MQ=255)                                                                     tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTa                                                <  2:80292/136‑1 (MQ=255)                                                                      cTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTAGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACagt                                            <  2:1231387/139‑1 (MQ=255)                                                                       tttGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACagta                                           >  1:635803/1‑139 (MQ=255)                                                                         tGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACagtagt                                         >  2:1148814/1‑139 (MQ=255)                                                                               tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCag                                                                                       <  1:323737/87‑1 (MQ=255)                                                                               tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCag                                                                                       >  2:323737/1‑87 (MQ=255)                                                                               tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCAtt                                                                                <  2:920413/94‑1 (MQ=255)                                                                               tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCAtt                                                                                >  1:920413/1‑94 (MQ=255)                                                                               tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTa                                                          >  1:1266656/1‑116 (MQ=255)                                                                               tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTa                                                          <  2:1266656/116‑1 (MQ=255)                                                                               tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAAc                                   >  1:669719/1‑139 (MQ=255)                                                                                gAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACg                                  <  2:669719/139‑1 (MQ=255)                                                                                  gTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGt                                <  2:1373617/139‑1 (MQ=255)                                                                                  gTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGt                                <  2:1477145/139‑1 (MQ=255)                                                                                                    tGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGACCAATActgtctgt              >  1:905706/1‑139 (MQ=255)                                                                                                        cTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGcc          <  1:1148814/139‑1 (MQ=255)                                                                                                                tGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCAtt                                                                                >  1:1084171/1‑61 (MQ=255)                                                                                                                tGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCAtt                                                                                <  2:1084171/61‑1 (MQ=255)                                                                                                                tGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACa                                              >  2:1292192/1‑95 (MQ=255)                                                                                                                tGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACa                                              <  1:1292192/95‑1 (MQ=255)                                                                                                                tGATTTCAGAAATATATATATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAaga  >  2:1443003/1‑139 (MQ=255)                                                                                                                              tatataTCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTAc                                               >  1:1091425/1‑80 (MQ=255)                                                                                                                              tatataTCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTAc                                               <  2:1091425/80‑1 (MQ=255)                                                                                                                                  |                                                                                                                          CAGTTCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAAC‑AGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGA  >  NC_000913/151907‑152156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑