Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	183,620	G→A	*386* (TAG→TAA)	cdaR →	carbohydrate diacid regulon transcriptional regulator, autoregulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	183,620	0	G	A	100.0%	246.8 / NA	76	*386* (TAG→TAA)	cdaR	carbohydrate diacid regulon transcriptional regulator, autoregulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/35);  total (41/35)

AAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCT  >  NC_000913/183485‑183750                                                                                                                                           |                                                                                                                                   aaaGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTa                                                                                                                                <  1:543751/139‑1 (MQ=255)       gttgttTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGtt                                                                                                                          <  1:761018/139‑1 (MQ=255)        ttgttTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCg                                                                                                                            >  1:1253484/1‑139 (MQ=255)          gttTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAg                                                                                                                       >  2:359141/1‑139 (MQ=255)                   tCGTAATACCCTGGAGTATCGGCTTAATCGTACATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTa                                                                                                                                >  2:959961/1‑121 (MQ=255)                   tCGTAATACCCTGGAGTATCGGCTTAATCGTACATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTa                                                                                                                                <  1:959961/121‑1 (MQ=255)                   tCGTAATACCCGGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAggcg                                                                                                              >  1:851989/1‑139 (MQ=255)                    cGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAggcgg                                                                                                             >  1:1275818/1‑139 (MQ=255)                     gTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAggcggc                                                                                                            >  1:1356816/1‑139 (MQ=255)                           cccTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGc                                                                                                      <  2:930513/139‑1 (MQ=255)                            ccTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGc                                                                                                      >  1:67059/1‑138 (MQ=255)                            ccTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGc                                                                                                      <  2:67059/138‑1 (MQ=255)                              tGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAgg                                                                                                                >  1:1380445/1‑126 (MQ=255)                              tGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAgg                                                                                                                <  2:1380445/126‑1 (MQ=255)                                gAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTAGCTCAtt                                                                                                 >  2:883818/1‑139 (MQ=255)                                   tATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTccc                                                                                              >  2:910970/1‑139 (MQ=255)                                     tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGTGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTgg                                                                                                       >  1:1186988/1‑128 (MQ=255)                                     tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGTGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTgg                                                                                                       <  2:1186988/128‑1 (MQ=255)                                     tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGgcgc                                                                                                          >  1:158840/1‑125 (MQ=255)                                     tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGgcgc                                                                                                          <  2:158840/125‑1 (MQ=255)                                     tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                            >  2:703480/1‑139 (MQ=255)                                     tCGGCTTAATCGTATATCGAAACTGACCGTGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                            >  2:981706/1‑139 (MQ=255)                                      cGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGc                                                                                                      <  2:524355/128‑1 (MQ=255)                                      cGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGc                                                                                                      >  1:524355/1‑128 (MQ=255)                                         cTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTc                                                                                           <  2:1253484/139‑1 (MQ=255)                                              tCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                   <  1:910970/139‑1 (MQ=255)                                              tCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                   <  1:703480/139‑1 (MQ=255)                                              tCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                   <  2:1356816/139‑1 (MQ=255)                                              tCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                   <  2:851989/139‑1 (MQ=255)                                              tCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTAGCTCATTCCCCTCAGCCCGaa                                                                                   <  1:883818/139‑1 (MQ=255)                                                  atatCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGt                                                                               <  2:1275818/139‑1 (MQ=255)                                                        gAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGATGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGTTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAggg                                                                         >  2:1222638/1‑139 (MQ=255)                                                           cTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTag                                                                             <  2:1422199/132‑1 (MQ=255)                                                           cTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTag                                                                             >  1:1422199/1‑132 (MQ=255)                                                              aCCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTa                                                                                                                                <  1:828184/78‑1 (MQ=255)                                                              aCCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTa                                                                                                                                >  2:828184/1‑78 (MQ=255)                                                              aCCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                   >  1:637896/1‑139 (MQ=255)                                                               ccGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGAGTAGAGGGGAATGcc                                                                  <  1:981706/139‑1 (MQ=255)                                                                   gCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAgggg                                                                        <  2:1275627/129‑1 (MQ=255)                                                                   gCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAgggg                                                                        >  1:1275627/1‑129 (MQ=255)                                                                        aTTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAAc                                                         >  2:320999/1‑139 (MQ=255)                                                                        aTTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAAc                                                         >  1:1410664/1‑139 (MQ=255)                                                                             ggCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                           <  1:1436080/84‑1 (MQ=255)                                                                             ggCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                           >  2:1436080/1‑84 (MQ=255)                                                                             ggCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTg                                                                                                        <  1:304927/87‑1 (MQ=255)                                                                             ggCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTg                                                                                                        >  2:304927/1‑87 (MQ=255)                                                                             ggCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                   <  1:46849/124‑1 (MQ=255)                                                                             ggCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                   >  2:46849/1‑124 (MQ=255)                                                                                  ttttGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCGCTCAGCCCCaa                                                                                   <  1:853167/103‑1 (MQ=255)                                                                                  ttttGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                   >  2:853167/1‑103 (MQ=255)                                                                                  ttttGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTag                                                                             >  2:735357/1‑109 (MQ=255)                                                                                  ttttGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTag                                                                             <  1:735357/109‑1 (MQ=255)                                                                                  ttttGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCg                                               >  2:1031614/1‑139 (MQ=255)                                                                                     tgatgaCAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGa                                            <  2:638106/139‑1 (MQ=255)                                                                                            aGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGtt                                                                                                                          <  1:1274122/54‑1 (MQ=255)                                                                                            aGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGtt                                                                                                                          >  2:1274122/1‑54 (MQ=255)                                                                                                             tgtgGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTc                       >  1:1334409/1‑139 (MQ=255)                                                                                                                  cGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGc                                                                                                      <  2:987533/55‑1 (MQ=255)                                                                                                                  cGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGc                                                                                                      >  1:987533/1‑55 (MQ=255)                                                                                                                  cGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTag                                                                             >  2:1403370/1‑80 (MQ=255)                                                                                                                  cGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTag                                                                             <  1:1403370/80‑1 (MQ=255)                                                                                                                  cGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGAt                  >  1:317664/1‑139 (MQ=255)                                                                                                                  cGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGAt                  >  1:297515/1‑139 (MQ=255)                                                                                                                     tACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTg                                                                                                        >  2:1393579/1‑50 (MQ=255)                                                                                                                     tACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTg                                                                                                        <  1:1393579/50‑1 (MQ=255)                                                                                                                     tACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCgg               >  2:1012258/1‑139 (MQ=255)                                                                                                                     tACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCgg               >  2:899183/1‑139 (MQ=255)                                                                                                                       cAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                           <  2:31011/45‑1 (MQ=255)                                                                                                                       cAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                           >  1:31011/1‑45 (MQ=255)                                                                                                                       cAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCt             >  2:682874/1‑139 (MQ=255)                                                                                                                              gtGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                                     <  2:182745/60‑1 (MQ=255)                                                                                                                              gtGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                                     >  1:182745/2‑61 (MQ=255)                                                                                                                                gAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAAtctc     >  2:1164926/1‑139 (MQ=255)                                                                                                                                 aaGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCg    <  2:1334409/139‑1 (MQ=255)                                                                                                                                   gagCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCt  <  2:278722/139‑1 (MQ=255)                                                                                                                                   gagCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCt  <  1:1222638/139‑1 (MQ=255)                                                                                                                                           |                                                                                                                                   AAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGT‑‑‑TGCTGCTGTATGTGGCGTTACAACTGGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCT  >  NC_000913/183485‑183750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑