Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,137,229	A→G	S143S (AGT→AGC)	yijF ←	DUF1287 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,137,229	0	A	G	100.0%	208.4 / NA	58	S143S (AGT→AGC)	yijF	DUF1287 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/25);  total (33/25)

CCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCACTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCAGCAAAATTC  >  NC_000913/4137099‑4137361                                                                                                                                    |                                                                                                                                     ccTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGtt                                                                                                                              <  2:790954/139‑1 (MQ=255)     cgcgcCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCt                                                                                                                            <  2:621778/137‑1 (MQ=255)     cgcgcCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCt                                                                                                                            >  1:621778/1‑137 (MQ=255)     cgcgcCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTg                                                                                                                          >  2:978469/1‑139 (MQ=255)     cgcgcCTGCGCCAATGTTATGTATCACCAGCGGCATACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTg                                                                                                                          >  1:11587/1‑139 (MQ=255)               ccAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc                                                                                                                <  2:900055/139‑1 (MQ=255)                               ccAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATggcg                                                                                                >  2:569937/1‑139 (MQ=255)                               ccAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATggcg                                                                                                >  2:104027/1‑139 (MQ=255)                                 aGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATggcggc                                                                                              >  1:671935/1‑139 (MQ=255)                                  gcggcgTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCt                                                                                             >  2:418375/1‑139 (MQ=255)                                    ggcgTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTaa                                                                                           >  2:1388139/1‑139 (MQ=255)                                      cgTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTgggg                                                                                                                 >  2:674425/1‑115 (MQ=255)                                      cgTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTgggg                                                                                                                 <  1:674425/115‑1 (MQ=255)                                       gTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAAcc                                                                                        <  2:587812/139‑1 (MQ=255)                                        tACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTggg                                                                                                                  <  1:1046997/112‑1 (MQ=255)                                        tACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTggg                                                                                                                  >  2:1046997/1‑112 (MQ=255)                                        tACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc                                                                                                                <  2:1240338/114‑1 (MQ=255)                                        tACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc                                                                                                                >  1:1240338/1‑114 (MQ=255)                                          ccGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAgg                                                                                     >  2:1170678/1‑139 (MQ=255)                                             tCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCa                                                                                       >  2:30055/1‑134 (MQ=255)                                             tCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCa                                                                                       <  1:30055/134‑1 (MQ=255)                                                             cTGAAACCACCCCAATATGTGCCAGCGCGTTATCCAGTCGCCAGGAGACAATATCG‑TCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGAGGCTAAACCAGGTTTCCAGATTAGGCACACg                                                                  >  1:353661/1‑139 (MQ=255)                                                                  accaccCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACgg                                                              >  1:1068845/1‑139 (MQ=255)                                                                  accaccCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATg                                                                                                   <  1:1154699/101‑1 (MQ=255)                                                                  accaccCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATg                                                                                                   >  2:1154699/1‑101 (MQ=255)                                                                   ccaccCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTg                                                            <  1:1170678/139‑1 (MQ=255)                                                                     accCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCTACGGTGAt                                                          <  2:957546/139‑1 (MQ=255)                                                                      ccccAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc                                                                                                                <  2:48047/84‑1 (MQ=255)                                                                      ccccAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc                                                                                                                >  1:48047/1‑84 (MQ=255)                                                                       cccAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGTTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCg                                                        <  2:11587/139‑1 (MQ=255)                                                                       cccAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCg                                                        >  2:217583/1‑139 (MQ=255)                                                                       cccAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCg                                                        >  2:1397760/1‑139 (MQ=255)                                                                       cccAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCg                                                        <  1:104027/139‑1 (MQ=255)                                                                                tgCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTg                                               >  1:558569/1‑139 (MQ=255)                                                                                 gCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCt                                                                                                                                    >  1:1249217/1‑53 (MQ=255)                                                                                 gCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCa                                                                                       <  1:386930/98‑1 (MQ=255)                                                                                 gCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCa                                                                                       >  2:386930/1‑98 (MQ=255)                                                                                      cccGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAAc                                                                                         >  2:925421/1‑91 (MQ=255)                                                                                      cccGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAAc                                                                                         <  1:925421/91‑1 (MQ=255)                                                                                      cccGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGttt                                                                                  >  2:720957/1‑98 (MQ=255)                                                                                      cccGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGttt                                                                                  <  1:720957/98‑1 (MQ=255)                                                                                          ttATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAg                                                                         >  2:1281188/1‑103 (MQ=255)                                                                                          ttATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAg                                                                         <  1:1281188/103‑1 (MQ=255)                                                                                          ttATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCt                                     >  2:551017/1‑139 (MQ=255)                                                                                                  tCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCAcc                                                                    <  1:1343118/100‑1 (MQ=255)                                                                                                  tCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCAcc                                                                    >  2:1343118/1‑100 (MQ=255)                                                                                                    gCCAGGAGACAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCa                           >  2:1242306/1‑139 (MQ=255)                                                                                                         gagaCAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGttt                                                                                  >  1:1210977/1‑79 (MQ=255)                                                                                                         gagaCAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGttt                                                                                  <  2:1210977/79‑1 (MQ=255)                                                                                                           gaCAATATCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGc                    <  2:671935/139‑1 (MQ=255)                                                                                                                tatCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAAc                                                                                         >  1:809960/1‑65 (MQ=255)                                                                                                                tatCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAAc                                                                                         <  2:809960/65‑1 (MQ=255)                                                                                                                tatCG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTa               <  1:353503/139‑1 (MQ=255)                                                                                                                   cG‑CCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTc            >  2:751805/1‑139 (MQ=255)                                                                                                                      cccGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCgg                                          >  1:701115/1‑107 (MQ=255)                                                                                                                      cccGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCgg                                          <  2:701115/107‑1 (MQ=255)                                                                                                                         gCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCAGCaa       >  1:521653/1‑139 (MQ=255)                                                                                                                              aTAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCAGCAAAAttc  <  1:1242306/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                     CCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCG‑CCCGCTTGATAGTCACTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCAGCAAAATTC  >  NC_000913/4137099‑4137361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑