| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,399,985 | (C)7→8 | coding (252/516 nt) | ogt ← | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,399,978 | 1 | . | C | 90.9% | 68.5 / 0.4 | 22 | coding (259/516 nt) | ogt | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (9/11); total (10/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCC > NC_000913/1399844‑1400111 | aTTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑cccc < 2:211886/139‑1 (MQ=255) aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccg < 2:413722/139‑3 (MQ=255) aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccg < 2:192180/139‑3 (MQ=255) aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTACGTAGTATTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccg > 2:396955/1‑137 (MQ=255) cGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccgtag < 1:419204/139‑6 (MQ=255) gTTTGCCGCACCAACGACACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGc < 1:702387/139‑1 (MQ=255) aCGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGGTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTa > 1:627194/1‑139 (MQ=255) gcgccgcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCaataa > 1:262675/1‑139 (MQ=255) cgccgcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCaataat < 2:427106/139‑1 (MQ=255) gccgcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATg > 1:443363/1‑139 (MQ=255) ccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCaa < 2:443363/139‑1 (MQ=255) ttGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt < 1:599452/139‑1 (MQ=255) ttGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt < 2:262675/139‑1 (MQ=255) aaTGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTa > 1:312579/1‑139 (MQ=255) ttACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGc < 2:811195/139‑1 (MQ=255) tGCCCGCAGGGGATAGTGCGTAGTGTTTTCTAGACTTCGCGCTGAAATGGCGAC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAACATTCACGAAGCTTGTCGCTTAAACCGCCTGGa < 2:739910/139‑1 (MQ=255) gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGa > 2:360754/1‑72 (MQ=255) gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGa < 1:360754/72‑1 (MQ=255) ccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCaata > 1:687880/1‑82 (MQ=255) ccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCaata < 2:687880/82‑1 (MQ=255) cAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccc < 2:767458/54‑1 (MQ=255) cAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccc > 1:767458/1‑54 (MQ=255) aTAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGaa > 2:200324/1‑139 (MQ=255) tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccg < 2:735812/46‑3 (MQ=255) tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑ccccccccg > 1:735812/1‑44 (MQ=255) gCGTAGTGTTTTCCAGACTTCGCGCTGAAATAGCGAC‑CCCCCCCGTAGAAGTGGGAAGCGTTTCAATAATGCTACGATTACCGGCAAAATATTCACGAAGCTTGACTCTTAAACTGACTGGATTGGTGGCAGTAATgcg > 2:24204/1‑139 (MQ=255) aGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaa < 1:930108/108‑1 (MQ=255) aGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaa > 2:930108/1‑108 (MQ=255) aGACTTCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCcttctt > 2:67813/1‑139 (MQ=255) ttCGCGCTGAAATGGCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCg < 1:200324/139‑1 (MQ=255) ggCG‑TCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTcc < 1:67813/139‑1 (MQ=255) | ATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCG‑T‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCC > NC_000913/1399844‑1400111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |