| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,474,138 | (A)6→7 | coding (15/594 nt) | bdcR → | transcriptional repressor for divergent bdcA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,474,132 | 1 | . | A | 96.8% | 197.2 / ‑1.3 | 63 | coding (9/594 nt) | bdcR | transcriptional repressor for divergent bdcA |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base A (35/26); total (35/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGG > NC_000913/4474013‑4474263 | ccACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTAATATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGtt > 1:163934/1‑139 (MQ=255) aCCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTcc > 2:288548/1‑139 (MQ=255) tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGtt > 2:697300/1‑139 (MQ=255) tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGtt > 1:415833/1‑139 (MQ=255) aaaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAAtctc < 2:636216/105‑1 (MQ=255) aaaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAAtctc > 1:636216/1‑105 (MQ=255) aaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGc > 2:477156/1‑139 (MQ=255) cgcCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGttt < 2:430899/125‑1 (MQ=255) cgcCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGttt > 1:430899/1‑125 (MQ=255) tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGttt > 2:541784/1‑112 (MQ=255) tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGttt < 1:541784/112‑1 (MQ=255) tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGc > 1:118005/1‑139 (MQ=255) cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa > 2:235626/1‑139 (MQ=255) tatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTc > 2:194226/1‑105 (MQ=255) tatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTc < 1:194226/105‑1 (MQ=255) tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGttt > 1:317829/1‑96 (MQ=255) tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGttt < 2:317829/96‑1 (MQ=255) tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg < 1:704791/106‑1 (MQ=255) tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg > 2:704791/1‑106 (MQ=255) aCATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCa > 1:421706/1‑139 (MQ=255) cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGa < 2:898481/88‑1 (MQ=255) cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGa > 1:898481/1‑88 (MQ=255) cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaa > 1:222972/1‑139 (MQ=255) cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaa > 2:19721/1‑139 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAAtctc > 2:820201/1‑66 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAAtctc < 1:820201/66‑1 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTcg < 2:898152/67‑1 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTcg > 1:898152/1‑67 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa < 1:19721/139‑1 (MQ=255) gCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAAtctc < 1:279837/65‑1 (MQ=255) gCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAAtctc > 2:279837/1‑65 (MQ=255) aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAgg < 2:738117/73‑1 (MQ=255) aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAgg > 1:738117/1‑73 (MQ=255) aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAgg > 1:129959/1‑139 (MQ=255) aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAgg > 2:740601/1‑139 (MQ=255) aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAgg > 2:753314/1‑139 (MQ=255) tACAAAACTGTTTTAACTCTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAgg > 2:421545/1‑71 (MQ=255) tACAAAACTGTTTTAACTCTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAgg < 1:421545/71‑1 (MQ=255) aaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGAt < 1:477156/139‑1 (MQ=255) cTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGc > 1:338992/1‑139 (MQ=255) ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGAt > 1:759312/1‑134 (MQ=255) ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGAt < 2:759312/134‑1 (MQ=255) ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt < 2:222972/139‑1 (MQ=255) tAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAg < 1:288548/139‑1 (MQ=255) tAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAg < 2:384773/139‑1 (MQ=255) ttCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTg > 2:919088/1‑139 (MQ=255) cTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAg > 2:121331/1‑132 (MQ=255) cTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAg < 1:121331/132‑1 (MQ=255) cTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCt < 2:338992/139‑1 (MQ=255) tGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa > 2:366834/1‑94 (MQ=255) tGTCAACAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa < 1:366834/94‑1 (MQ=255) aaCAGGTTTTGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGt < 2:242171/139‑1 (MQ=255) aaCAGGTTTTGTAATGGTCACT‑AA‑AAACACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGt < 2:118005/139‑1 (MQ=255) aCAGGTTTTGTAATGGTCACT‑AAGAAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGtt < 2:163934/139‑1 (MQ=255) ttGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAtt > 1:441517/1‑139 (MQ=255) ttGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAt < 2:27467/138‑1 (MQ=255) ttGTAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAt > 1:27467/1‑138 (MQ=255) tAATGGTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATc < 2:441517/139‑1 (MQ=255) ggTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCttttt > 2:344590/1‑86 (MQ=255) ggTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCttttt < 1:344590/86‑1 (MQ=255) ggTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGt > 2:714688/1‑124 (MQ=255) ggTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGt < 1:714688/124‑1 (MQ=255) ggTCACTAAA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTgg > 1:673535/1‑139 (MQ=255) | CCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AA‑AAA‑ACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGG > NC_000913/4474013‑4474263 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |