Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,276	G→A	*392* (TAG→TAA)	ubiF →	2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,276	0	G	A	100.0%	29.9 / NA	11	*392* (TAG→TAA)	ubiF	2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/8);  total (3/8)

GGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAGCCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAA  >  NC_000913/696163‑696387                                                                                                                    |                                                                                                                 ggATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGc                                                                                        <  2:568985/139‑1 (MQ=255)  gATTCAGCAATAATCTGCCACCCCTGGGTTTTA‑GCCGAATCCCGGGGTAATGGGCGCCGAGCCTGCCTGCGTGGTTAAACGTTAGGGCGTTG‑AATAATCGTTAAGGGTTGTACCCTAAAAAC‑TTGCCGGAATGCGTccc                                                                                       >  2:46156/1‑139 (MQ=255)            taatCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGt                                                                             <  1:251169/139‑1 (MQ=255)                                      gTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATc                                                   >  2:817620/1‑139 (MQ=255)                                        aaTCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCg                                                 <  1:817620/139‑1 (MQ=255)                                        aaTCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCg                                                 <  2:718179/139‑1 (MQ=255)                                                    aTGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGAt                                     <  1:46156/139‑1 (MQ=255)                                                                  tGCTGGCGTGTTGAAACGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACa                       <  2:777744/139‑1 (MQ=255)                                                                            ttGAAACGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGa             >  2:605861/1‑139 (MQ=255)                                                                                  cGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATtga       <  2:195517/139‑1 (MQ=255)                                                                                                         t‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATtgaatgaa  >  1:74698/1‑122 (MQ=255)                                                                                                         t‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATtgaatgaa  <  2:74698/122‑1 (MQ=255)                                                                                                                    |                                                                                                                 GGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAGCCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAA  >  NC_000913/696163‑696387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑