Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	915,730	T→C	K106K (AAA→AAG)	aqpZ ←	aquaporin Z

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	915,730	0	T	C	100.0%	106.6 / NA	30	K106K (AAA→AAG)	aqpZ	aquaporin Z
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/16);  total (14/16)

GGATCACCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTTTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCATAAACCAATAGTGACCG  >  NC_000913/915600‑915854                                                                                                                                    |                                                                                                                             ggATCACCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACt                                                                                                                      <  1:783789/139‑1 (MQ=255)                             tACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCG‑CAAAACCCTTAGCAGCAAAACCCCCTGCCTGCGCCGCAAAAACCCG‑CCTAACACTGGGAAATAAATAccgccggcccggctgaa                                                                                         >  2:187726/1‑126 (MQ=255)                                cAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGcaaca                                                                                       >  1:848786/1‑139 (MQ=255)                                   ttCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATa                                                                                    >  1:304087/1‑139 (MQ=255)                                   ttCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATa                                                                                    >  1:312986/1‑139 (MQ=255)                                     cAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTgg                                                                                                                    >  1:277452/1‑105 (MQ=255)                                     cAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTgg                                                                                                                    <  2:277452/105‑1 (MQ=255)                                       aCTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTgg                                                                                                                    >  2:590116/1‑103 (MQ=255)                                       aCTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTgg                                                                                                                    <  1:590116/103‑1 (MQ=255)                                       aCTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAACTAAATACAGCAGCGCCGCTGCAACAATAccgc                                                                                >  2:275921/1‑139 (MQ=255)                                         tACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATAccgccg                                                                              <  2:312986/139‑1 (MQ=255)                                              gcgcGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAAc                                                                         <  2:304087/139‑1 (MQ=255)                                               cgcgGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAAcc                                                                        >  2:878897/1‑139 (MQ=255)                                                gcgAAAAGAAGGAAATAACCGCCGGGTGATTCCTC‑CCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTg                                                                      <  1:187726/139‑1 (MQ=255)                                                 cgGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTg                                                                      <  1:783400/139‑1 (MQ=255)                                                        cATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAAtt                                                               >  2:824210/1‑139 (MQ=255)                                                           ggAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACg                                                            <  1:530677/139‑1 (MQ=255)                                                                     ccTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGa                                                  >  2:108239/1‑139 (MQ=255)                                                                              tGCTCGCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCa                                                                  >  2:326415/1‑114 (MQ=255)                                                                              tGCTCGCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCa                                                                  <  1:326415/114‑1 (MQ=255)                                                                                   gCCATAACCGTTAGAAGCAAAA‑CCGCTGTCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATGCAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGa                                    >  1:576936/1‑139 (MQ=255)                                                                                   gCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGa                                    >  1:150342/1‑139 (MQ=255)                                                                                               aGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCa                        <  1:824210/139‑1 (MQ=255)                                                                                               aGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCa                        <  2:576936/139‑1 (MQ=255)                                                                                               aGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCa                        <  2:150342/139‑1 (MQ=255)                                                                                                    cAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGGCAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCa                   <  1:417152/139‑1 (MQ=255)                                                                                                               ggctgCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCATAAACCAATa         >  1:476344/1‑139 (MQ=255)                                                                                                                     cgcgTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCATAAACCAATAGTGAcc   >  2:212415/1‑139 (MQ=255)                                                                                                                      gcgTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCATAAACCAATAGTGAccg  <  2:476344/139‑1 (MQ=255)                                                                                                                      gcgTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCATAAACCAATAGTGAccg  <  2:868877/139‑1 (MQ=255)                                                                                                                      gcgTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCATAAACCAATAGTGAccg  <  1:108239/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                             GGATCACCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAA‑CCGCTGGCTGCCGCGTCAAAACCCGTTTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCATAAACCAATAGTGACCG  >  NC_000913/915600‑915854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑