Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,014,259	Δ1 bp	intergenic (‑20/+28)	yqeB ← / ← yqeC	XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold/putative selenium‑dependent hydroxylase accessory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,014,258	0	C	.	100.0%	204.6 / NA	49	intergenic (‑19/+29)	yqeB/yqeC	XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold/putative selenium‑dependent hydroxylase accessory protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (22/27);  total (22/27)

CGCGCACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCA  >  NC_000913/3014122‑3014392                                                                                                                                         |                                                                                                                                       cgcgcACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATct                                                                                                                                       >  1:1422998/1‑137 (MQ=255) cgcgcACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATct                                                                                                                                       <  2:1422998/138‑2 (MQ=255)     cACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAg                                                                                                                                 <  2:1478399/139‑1 (MQ=255)        tAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTaa                                                                                                                              <  1:1442146/139‑1 (MQ=255)                           cGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCgt                                                                                                           <  1:522824/139‑2 (MQ=255)                             gggAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCgtta                                                                                                         <  2:227989/139‑4 (MQ=255)                                aGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAAt                                                                                                       >  2:666605/1‑138 (MQ=255)                                aGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAAt                                                                                                       <  1:666605/138‑1 (MQ=255)                                  tCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTca                                                                                                    >  1:698017/1‑139 (MQ=255)                                    gAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTcaca                                                                                                  >  2:891015/1‑139 (MQ=255)                                       ccTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAAt                                                                                               <  1:891015/139‑1 (MQ=255)                                                      aTTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCa                                                                                <  2:698017/139‑1 (MQ=255)                                                       tttGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGa                                                                                                                        <  2:212834/98‑1 (MQ=255)                                                       tttGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGa                                                                                                                        >  1:212834/1‑98 (MQ=255)                                                        ttGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg                                                                              <  2:157113/139‑1 (MQ=255)                                                          gcgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGAt                                                                            >  2:466005/1‑139 (MQ=255)                                                           cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                           <  2:1670062/139‑1 (MQ=255)                                                           cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                           <  1:1066205/139‑1 (MQ=255)                                                                        ggACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                             <  1:1419110/124‑1 (MQ=255)                                                                        ggACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                             >  2:1419110/1‑124 (MQ=255)                                                                           cAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATAcc                                                                                                                   <  2:1450367/83‑1 (MQ=255)                                                                           cAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATAcc                                                                                                                   >  1:1450367/1‑83 (MQ=255)                                                                           cAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGccc                                                           >  1:1618542/1‑139 (MQ=255)                                                                            aaTTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCgt                                                                                                           >  2:361949/1‑89 (MQ=255)                                                                            aaTTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCgt                                                                                                           <  1:361949/90‑2 (MQ=255)                                                                                      ttCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAAc                                                                                                                             >  1:314656/1‑62 (MQ=255)                                                                                      ttCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAAc                                                                                                                             <  2:314656/62‑1 (MQ=255)                                                                                        cGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                           <  1:1525313/110‑1 (MQ=255)                                                                                        cGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                           >  2:1525313/1‑110 (MQ=255)                                                                                               tGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTAcgtc                                       >  2:471687/1‑139 (MQ=255)                                                                                                gCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTAcgtcg                                      >  2:485038/1‑139 (MQ=255)                                                                                                gCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTAcgtcg                                      >  2:328299/1‑139 (MQ=255)                                                                                                      tctGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGtgttgt                                <  1:466005/139‑1 (MQ=255)                                                                                                       ctGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCct                                              <  1:1330828/124‑1 (MQ=255)                                                                                                       ctGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCct                                              >  2:1330828/1‑124 (MQ=255)                                                                                                        tgtgAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                             <  1:64564/92‑1 (MQ=255)                                                                                                        tgtgAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                             >  2:64564/1‑92 (MQ=255)                                                                                                          tgAAAATATTCATACCAACCTCAATGTATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCa                            <  2:1541658/139‑1 (MQ=255)                                                                                                                  ttCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                                        <  2:976177/71‑1 (MQ=255)                                                                                                                  ttCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgagcg                                                                                        >  1:976177/1‑71 (MQ=255)                                                                                                                  ttCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCa                            <  1:603406/131‑1 (MQ=255)                                                                                                                  ttCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCa                            >  2:603406/1‑131 (MQ=255)                                                                                                                           aCCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTg                                                                                            <  2:1642574/58‑1 (MQ=255)                                                                                                                           aCCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTg                                                                                            >  1:1642574/1‑58 (MQ=255)                                                                                                                           aCCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCg           >  1:484561/1‑139 (MQ=255)                                                                                                                            ccTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg                                                                              >  2:247836/1‑71 (MQ=255)                                                                                                                            ccTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg                                                                              <  1:247836/71‑1 (MQ=255)                                                                                                                            ccTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGc          >  2:1587020/1‑139 (MQ=255)                                                                                                                             cTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCt         <  1:471687/139‑1 (MQ=255)                                                                                                                                aaTGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTtg      <  1:328299/139‑1 (MQ=255)                                                                                                                                    gATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCa  <  1:485038/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CGCGCACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCA  >  NC_000913/3014122‑3014392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑