| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 401,548 | (G)5→4 | coding (163/261 nt) | iraP → | anti‑RssB factor, RpoS stabilzer during Pi starvation, anti‑adapter protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 401,544 | 0 | G | . | 98.2% | 221.5 / ‑2.6 | 55 | coding (159/261 nt) | iraP | anti‑RssB factor, RpoS stabilzer during Pi starvation, anti‑adapter protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base . (27/27); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
TTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCAT > NC_000913/401410‑401673 | ttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTagag > 2:814883/1‑135 (MQ=255)ttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTagag < 1:814883/135‑1 (MQ=255) cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg > 2:762291/1‑125 (MQ=255) cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg < 1:762291/127‑3 (MQ=255) cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTa > 1:290458/1‑139 (MQ=255) aaaGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGcc < 1:441645/139‑1 (MQ=255) gaagaagaGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGTAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGa < 2:93586/139‑1 (MQ=255) gaagaagaGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGa < 2:290458/139‑1 (MQ=255) tgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATa < 2:567329/139‑1 (MQ=255) gAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgctgct > 1:1496726/1‑139 (MQ=255) tttGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGcgcg < 2:1496726/139‑1 (MQ=255) tGGAGATTATCGTCACTGCAATGCTTCGCAATATGTCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt > 1:944231/1‑139 (MQ=255) tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt > 2:1169497/1‑139 (MQ=255) tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt > 2:1009224/1‑139 (MQ=255) ggAGATTATCGTCACTGCAATGCTTCGCAATATGTCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAtt < 2:944231/139‑1 (MQ=255) tATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTaa > 1:141625/1‑139 (MQ=255) tATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTaa > 2:818895/1‑139 (MQ=255) cGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTga < 1:258313/104‑1 (MQ=255) cGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTga > 2:258313/1‑104 (MQ=255) tCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaagaag < 1:818895/139‑1 (MQ=255) cACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGt > 1:235985/1‑139 (MQ=255) cAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTg < 1:1169497/139‑1 (MQ=255) aaTGCTTCGCAATATGGTGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTCAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTaaa > 1:956392/1‑128 (MQ=255) aaTGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTaaa < 2:956392/128‑1 (MQ=255) aTGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacg < 1:136149/96‑1 (MQ=255) aTGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacg > 2:136149/1‑96 (MQ=255) aTGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGaa < 2:235985/139‑1 (MQ=255) aTGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGaa < 1:1009224/139‑1 (MQ=255) tGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAg > 2:1190432/1‑139 (MQ=255) gCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGc > 1:975903/1‑139 (MQ=255) cTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACgg < 1:1529588/102‑1 (MQ=255) cTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACgg > 2:1529588/1‑102 (MQ=255) cTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCa < 2:141625/139‑1 (MQ=255) aagagacagggCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGTGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATc < 2:398122/130‑1 (MQ=255) cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaagaag > 1:1237576/1‑125 (MQ=255) cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaagaag < 2:1237576/125‑1 (MQ=255) cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATcc > 2:243483/1‑139 (MQ=255) tGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATa < 2:786124/89‑1 (MQ=255) tGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATa > 1:786124/1‑89 (MQ=255) ggCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGt < 1:243483/139‑1 (MQ=255) ggCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGt > 1:1353380/1‑139 (MQ=255) cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAtt > 1:57250/1‑104 (MQ=255) cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAtt < 2:57250/104‑1 (MQ=255) cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGaa > 1:510725/1‑123 (MQ=255) cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGaa < 2:510725/123‑1 (MQ=255) aCCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑GCTGTACGAGGTAAAGCACGATGACAGAATTCCTGAAGAGGATACGGAGCTGATGGGCGAATAGGTAAAGAAGATATTGAAGCATCCTAGTCAGTaactggctctt > 1:398122/1‑130 (MQ=255) aCCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTaaa < 1:993203/102‑1 (MQ=255) aCCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTaaa > 2:993203/1‑102 (MQ=255) aCCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAAt < 2:761915/139‑1 (MQ=255) aGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCTCGATGCCa < 2:1085712/53‑1 (MQ=255) aGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCa > 1:1085712/1‑53 (MQ=255) gTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACa > 2:293551/1‑139 (MQ=255) attgatCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAtt > 2:1057565/1‑81 (MQ=255) attgatCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAtt < 1:1057565/81‑1 (MQ=255) atCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCAt > 2:702069/1‑139 (MQ=255) tCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATa > 2:1253717/1‑58 (MQ=255) tCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATa < 1:1253717/58‑1 (MQ=255) gTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTa < 2:664597/77‑1 (MQ=255) gTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTa > 1:664597/1‑77 (MQ=255) | TTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCAT > NC_000913/401410‑401673 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |