| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,250,629 | (C)5→6 | coding (210/1071 nt) | dhaK ← | dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,250,624 | 1 | . | C | 98.5% | 237.4 / ‑3.8 | 68 | coding (215/1071 nt) | dhaK | dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (33/34); total (34/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATG > NC_000913/1250493‑1250754 | cTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑ccc‑cccg < 1:1384550/139‑3 (MQ=255) gAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAc < 1:828983/139‑1 (MQ=255) tatCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGa < 2:1546247/139‑1 (MQ=255) cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAc < 1:91536/133‑1 (MQ=255) cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAc > 2:91536/1‑133 (MQ=255) cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGatat > 2:403621/1‑139 (MQ=255) gtgtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACca > 2:28147/1‑139 (MQ=255) gTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGc > 1:504603/1‑139 (MQ=255) cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc < 1:28147/139‑1 (MQ=255) cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc < 2:504603/139‑1 (MQ=255) tCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa > 2:432795/1‑97 (MQ=255) tCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa < 1:432795/97‑1 (MQ=255) cgccgcCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGTCCGGACAGGCCCC‑CCGAAAGCATccc > 1:962993/1‑92 (MQ=255) cgccgcCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGTCCGGACAGGCCCC‑CCGAAAGCATccc < 2:962993/92‑1 (MQ=255) cgccgcCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCAc > 2:985915/1‑139 (MQ=255) ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCAt < 2:1112232/114‑1 (MQ=255) ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCa < 2:738525/136‑1 (MQ=255) ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCa > 1:738525/1‑136 (MQ=255) ccgccACCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCAt > 1:1112232/1‑114 (MQ=255) cATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGAca > 1:1381829/1‑139 (MQ=255) aTCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc < 1:749652/91‑1 (MQ=255) aTCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc < 2:735803/91‑1 (MQ=255) aTCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc > 1:735803/1‑91 (MQ=255) aTCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGAcag > 1:30380/1‑139 (MQ=255) aTCAACTTGCATGGCGCATTCAAAGACTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc > 2:749652/1‑91 (MQ=255) cATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa < 1:558874/80‑1 (MQ=255) cATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa > 2:558874/1‑80 (MQ=255) tGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAg > 2:933398/1‑68 (MQ=255) tGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAg < 1:933398/68‑1 (MQ=255) tGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑c > 2:302906/1‑58 (MQ=255) tGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑c < 1:302906/58‑1 (MQ=255) gcgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAg > 1:199467/1‑66 (MQ=255) gcgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAg < 2:199467/66‑1 (MQ=255) cgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGa < 2:811101/79‑1 (MQ=255) cgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGa > 1:811101/1‑79 (MQ=255) cgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTc > 1:1106205/1‑139 (MQ=255) gcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa < 2:759912/74‑1 (MQ=255) gcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa > 1:759912/1‑74 (MQ=255) gcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTcc < 2:803564/139‑1 (MQ=255) cATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCt < 2:1381829/139‑1 (MQ=255) tCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCa > 2:904030/1‑139 (MQ=255) tCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCa > 2:74515/1‑139 (MQ=255) tCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑c < 1:1311605/50‑1 (MQ=255) tCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑c > 2:1311605/1‑50 (MQ=255) aaGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACa < 1:904030/139‑1 (MQ=255) aGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATccc < 1:444505/62‑1 (MQ=255) aGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATccc > 2:444505/1‑62 (MQ=255) tttATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccg < 1:768439/101‑1 (MQ=255) tttATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccg > 2:768439/1‑101 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCAt < 2:1007866/80‑1 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCAt > 1:1007866/1‑80 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑ccc‑cccg > 2:1590204/1‑44 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑ccc‑cccg < 1:1590204/46‑3 (MQ=255) gCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATGGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTc < 2:625383/139‑1 (MQ=255) gCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc < 1:534854/94‑1 (MQ=255) gCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc > 2:534854/1‑94 (MQ=255) gCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTc > 1:353913/1‑139 (MQ=255) gCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTc > 2:592219/1‑139 (MQ=255) cGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTcc < 2:469583/118‑1 (MQ=255) cGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTcc > 1:469583/1‑118 (MQ=255) gTCGGTGAGGTGAAATTTTCGCCCGGACAGG‑CCCACCGAAAGCATCCCCTGATCGa > 2:208873/1‑56 (MQ=255) gTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGa < 1:208873/56‑1 (MQ=255) tCGGTGAGGTGAAAATTTCGCCTGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTc > 1:988204/1‑136 (MQ=255) tCGGTGAGGTGAAAATTTCGCCTGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTc < 2:988204/136‑1 (MQ=255) tCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCggg < 2:421638/139‑1 (MQ=255) tCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCggg < 2:353913/139‑1 (MQ=255) tgaggtgaAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGAc < 2:1106205/139‑1 (MQ=255) ggtgaAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGc > 1:332568/1‑127 (MQ=255) ggtgaAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGc < 2:332568/127‑1 (MQ=255) gtgaAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATac > 1:1468170/1‑139 (MQ=255) aaaTTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCg < 2:1468170/139‑1 (MQ=255) cGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAg > 2:1158709/1‑106 (MQ=255) cGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAg < 1:1158709/106‑1 (MQ=255) gCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTg > 2:1119332/1‑139 (MQ=255) cGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATg < 1:403621/139‑1 (MQ=255) | CTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATG > NC_000913/1250493‑1250754 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |