| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,734,543 | (C)8→9 | coding (492/732 nt) | yfiH ← | UPF0124 family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,734,535 | 1 | . | C | 96.7% | 208.7 / ‑0.5 | 60 | coding (500/732 nt) | yfiH | UPF0124 family protein |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (27/31); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACG > NC_000913/2734399‑2734653 | gccgAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑cc < 2:1525801/139‑1 (MQ=255) tGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑cccccccccact < 2:1257620/139‑5 (MQ=255) aaCACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGaa < 1:220363/139‑1 (MQ=255) aCGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑cccccc > 1:1669344/1‑121 (MQ=255) aCGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑cccccc < 2:1669344/121‑1 (MQ=255) tCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGAc < 2:124017/139‑1 (MQ=255) cGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGAcc < 2:83422/139‑1 (MQ=255) cGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGAcc < 2:1388387/139‑1 (MQ=255) gCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAgg < 2:794940/139‑1 (MQ=255) cTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGAc < 1:491912/117‑1 (MQ=255) cTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGAc > 2:491912/1‑117 (MQ=255) tGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑CCCCCCCCACt < 2:1664729/99‑1 (MQ=255) tGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑CCCCCCCCACt > 1:1664729/1‑99 (MQ=255) atCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc < 2:1068110/115‑1 (MQ=255) atCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc > 1:1068110/1‑115 (MQ=255) ccGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGcc < 2:153934/118‑1 (MQ=255) ccGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGcc > 1:153934/1‑118 (MQ=255) gCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc > 1:1174948/1‑111 (MQ=255) gCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc < 2:1174948/111‑1 (MQ=255) aTACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGAtt < 2:1143675/139‑1 (MQ=255) aTCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc > 2:726478/1‑100 (MQ=255) aTCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc < 1:726478/100‑1 (MQ=255) aTCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGc > 1:284348/1‑139 (MQ=255) tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc > 1:108049/1‑93 (MQ=255) tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc < 2:108049/93‑1 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGaa > 2:709459/1‑74 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGaa < 1:709459/74‑1 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc > 2:1671364/1‑92 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc > 2:723241/1‑92 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc < 2:1128487/92‑1 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc < 1:723241/92‑1 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc < 1:1671364/92‑1 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc > 1:1128487/1‑92 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAgg > 1:1386900/1‑139 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCCACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAgg > 1:1500747/1‑106 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCCACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAgg < 2:1500747/106‑1 (MQ=255) gCTGAATGAAACCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAgg < 2:284348/139‑1 (MQ=255) aatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAAc > 2:1281956/1‑139 (MQ=255) gCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTc > 1:1479476/1‑139 (MQ=255) gCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGc > 1:1441832/1‑82 (MQ=255) gCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGc < 2:1441832/82‑1 (MQ=255) gCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTcc > 1:1620606/1‑139 (MQ=255) ttGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGaaa < 1:907010/124‑1 (MQ=255) ttGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGaaa > 2:907010/1‑124 (MQ=255) gTCTACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca < 1:1281956/139‑1 (MQ=255) tCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca < 2:1479476/139‑1 (MQ=255) tACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc > 1:1151341/1‑61 (MQ=255) tACTGCCATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc < 2:1151341/61‑1 (MQ=255) ccATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc < 1:1646975/56‑1 (MQ=255) ccATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc > 2:1646975/1‑56 (MQ=255) ccATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTc > 2:144818/1‑83 (MQ=255) ccATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTc < 1:144818/83‑1 (MQ=255) cATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca > 1:559848/1‑130 (MQ=255) cATAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca < 2:559848/130‑1 (MQ=255) aTAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAat > 1:378704/1‑75 (MQ=255) aTAAACGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAat < 2:378704/75‑1 (MQ=255) aaaCGCCTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTAt < 1:1540507/87‑1 (MQ=255) aaaCGCCTAGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTCTCCGGATTAt > 2:1540507/1‑87 (MQ=255) cTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc > 2:886813/1‑46 (MQ=255) cTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc < 1:886813/46‑1 (MQ=255) cTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACg < 1:1322054/130‑1 (MQ=255) cTCGCGAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACg > 2:1322054/1‑130 (MQ=255) cgAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc > 1:93200/1‑42 (MQ=255) cgAACCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc < 2:93200/42‑1 (MQ=255) | GCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACG > NC_000913/2734399‑2734653 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |