| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,728,118 | (C)8→9 | coding (1254/1455 nt) | xylB ← | xylulokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,728,110 | 1 | . | C | 97.6% | 137.4 / NA | 41 | D421G (GAT→GGT) | xylB | xylulokinase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (18/22); minor base A (1/0); total (19/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCA > NC_000913/3727972‑3728242 | ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CAt > 2:871246/1‑139 (MQ=255)ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CAt > 1:313087/1‑139 (MQ=255)ggCTGAGAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGTTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CAt > 1:1523849/1‑139 (MQ=255) aTAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CAT‑‑ccccc > 1:1383701/1‑139 (MQ=255) aaGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CAT‑‑ccccccc < 2:313087/139‑1 (MQ=255) gCATAACGCTGCGCATCTGTTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CAT‑‑cccccccccgta < 1:1529995/139‑5 (MQ=255) gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CAT‑‑cccccccccgta < 2:195454/139‑5 (MQ=255) tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACgg > 1:485531/1‑139 (MQ=255) tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CAT‑‑cccccccccgtac < 1:1434359/137‑6 (MQ=255) tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CAT‑‑cccccccccgtac > 2:1434359/1‑132 (MQ=255) aCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTa > 1:1469968/1‑139 (MQ=255) cTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATc < 2:1383701/139‑1 (MQ=255) cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAACCGAgctgct < 2:349937/139‑1 (MQ=255) tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGAcc < 1:871246/139‑1 (MQ=255) gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata < 2:485531/139‑1 (MQ=255) ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CAT‑‑cccc > 1:520182/1‑99 (MQ=255) ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CAT‑‑cccc < 2:520182/99‑1 (MQ=255) aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAg > 1:620171/1‑108 (MQ=255) aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAg < 2:620171/108‑1 (MQ=255) aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGCTATCCGCCAGCATCTGa > 1:920814/1‑139 (MQ=255) aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa > 1:391041/1‑139 (MQ=255) ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg < 2:1257150/139‑1 (MQ=255) gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc > 2:281784/1‑139 (MQ=255) cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAc < 2:154508/125‑1 (MQ=255) cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAc > 1:154508/1‑125 (MQ=255) gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccg < 2:391041/139‑1 (MQ=255) tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt < 2:1469968/139‑1 (MQ=255) tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt < 1:281784/139‑1 (MQ=255) tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt < 2:1070123/139‑1 (MQ=255) gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc > 1:1219579/1‑108 (MQ=255) gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc < 2:1219579/108‑1 (MQ=255) aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCCCTGAAATCCGCCAGCACCTGACGCCAGAACTCACTCCGCGCCCCCCCCCCaacaca > 1:1070123/1‑135 (MQ=255) cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CAT‑‑gccccccccg > 2:753676/1‑55 (MQ=255) cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCGGGCCCCA‑CAT‑‑cccccccccg < 1:753676/57‑3 (MQ=255) gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGGTATCCGCCAGCATCTGACGCCATACCTCACTACGCGCCCCGCCCCCAATCAAcaga > 1:1302924/1‑136 (MQ=255) gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt < 1:319257/98‑1 (MQ=255) gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt > 2:319257/1‑98 (MQ=255) gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTa > 1:556385/1‑139 (MQ=255) cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGAc > 2:806992/1‑75 (MQ=255) cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGAc < 1:806992/75‑1 (MQ=255) gATCTGCGCCCGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATA‑CCCCCCCCGTACGGCAATCAAGCACCTGATCGCTGGTATCCGCCCGCATCCGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACt > 1:1645099/1‑139 (MQ=255) aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAcc > 2:616107/1‑96 (MQ=255) aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg < 1:616107/97‑1 (MQ=255) cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg < 1:1549152/139‑1 (MQ=255) cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAg < 1:284579/58‑1 (MQ=255) cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAg > 2:284579/1‑58 (MQ=255) gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgc < 2:168650/113‑1 (MQ=255) gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgc > 1:168650/1‑113 (MQ=255) ccTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg > 2:999375/1‑130 (MQ=255) ccTTGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg < 1:999375/130‑1 (MQ=255) ttGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAg < 1:1454772/50‑1 (MQ=255) ttGCTGCGCCCAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAg > 2:1454772/1‑50 (MQ=255) gcgcCCAGCGCGGGCCCAACCCC‑‑CCCCCCCCGTCCGGTAATCGAGCTTCGTACCGCTGATATCCGCCAGCAACTGACGCCAATACTCACTTCGCGCGCCGCCCCCAGATGTCTTTACACTTTGCGGTTTAAGACCGCAg < 1:1266792/139‑1 (MQ=255) cccAGTGCTGGCCCCA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa < 2:1523849/139‑1 (MQ=255) ccA‑CATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa < 2:556385/139‑1 (MQ=255) | GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCA > NC_000913/3727972‑3728242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |