Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,072,060	G→A	L34L (CTG→TTG)	rutC ←	putative aminoacrylate deaminase, reactive intermediate detoxification, weak enamine/imine deaminase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,072,060	0	G	A	100.0%	181.6 / NA	58	L34L (CTG→TTG)	rutC	putative aminoacrylate deaminase, reactive intermediate detoxification, weak enamine/imine deaminase activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/28);  total (30/28)

GCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAA  >  NC_000913/1071929‑1072188                                                                                                                                    |                                                                                                                                  gCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                            >  1:1331925/1‑139 (MQ=255)   tGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGga                                                                                                                          >  1:441613/1‑139 (MQ=255)          ggTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATac                                                                                                                   >  1:856957/1‑139 (MQ=255)            tCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATacac                                                                                                                 <  1:738779/139‑1 (MQ=255)                  ccGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGcc                                                                                                           <  2:1331925/139‑1 (MQ=255)                          gTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACAt                                                                                                                     <  2:1440382/121‑1 (MQ=255)                          gTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACAt                                                                                                                     >  1:1440382/1‑121 (MQ=255)                                cccGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGc                                                                                            >  1:755864/1‑139 (MQ=255)                                     cgTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATAcacca                                                                                                               >  2:774990/1‑116 (MQ=255)                                     cgTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATAcacca                                                                                                               <  1:774990/116‑1 (MQ=255)                                                   tGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGc                                                                            >  2:582980/1‑136 (MQ=255)                                                   tGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGc                                                                            <  1:582980/136‑1 (MQ=255)                                                          aGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTgcg                                                                  >  2:1058335/1‑139 (MQ=255)                                                           gTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                            >  1:303540/1‑81 (MQ=255)                                                           gTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                            <  2:303540/81‑1 (MQ=255)                                                              tCCAGAACGT‑GCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGaa                                                                                      <  2:1492195/114‑1 (MQ=255)                                                              tCCAGAACGT‑GCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGaa                                                                                      >  1:1492195/1‑114 (MQ=255)                                                               ccAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGaa                                                                                      >  1:1490113/1‑114 (MQ=255)                                                               ccAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGaa                                                                                      <  2:1490113/114‑1 (MQ=255)                                                                    aCGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGaa                                                                                      <  1:953424/109‑1 (MQ=255)                                                                    aCGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGaa                                                                                      >  2:953424/1‑109 (MQ=255)                                                                      gTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCg                                                      >  2:1435980/1‑139 (MQ=255)                                                                        ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGaa                                                                                      >  2:827860/1‑105 (MQ=255)                                                                        ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGaa                                                                                      <  1:827860/105‑1 (MQ=255)                                                                        ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAg                                                                         >  2:330596/1‑118 (MQ=255)                                                                        ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCggg                                                    <  1:1435980/139‑1 (MQ=255)                                                                        ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACAGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAg                                                                         <  1:330596/118‑1 (MQ=255)                                                                                tGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTa                                            >  2:1267137/1‑139 (MQ=255)                                                                                     cTTTGGGTCATCGGCAAACAGCACGTTATTACGTTGATCAAAAGCCAACGTACCg                                                                                                                            >  1:972554/1‑55 (MQ=255)                                                                                     cTTTGGGTCATCGGCAAACAGCACGTTATTACGTTGATCAAAAGCCAACGTACCg                                                                                                                            <  2:972554/55‑1 (MQ=255)                                                                                       ttGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATc                                                                                                        <  2:1667755/73‑1 (MQ=255)                                                                                       ttGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATc                                                                                                        >  1:1667755/1‑73 (MQ=255)                                                                                          ggTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTgg                                  <  1:1267137/139‑1 (MQ=255)                                                                                                   cAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTaa                         <  2:856957/139‑1 (MQ=255)                                                                                                    aaaCAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAT‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTaaa                        <  1:1058335/139‑1 (MQ=255)                                                                                                          cACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCTTCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTG‑CAGCGGGAATAAtt                                             <  1:126842/111‑1 (MQ=255)                                                                                                          cACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTa                          >  1:961621/1‑131 (MQ=255)                                                                                                          cACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTa                          <  2:961621/131‑1 (MQ=255)                                                                                                          cACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTG‑CAGCGGGAATAAtt                                             >  2:126842/1‑111 (MQ=255)                                                                                                            cGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAg                                                                         <  2:943364/82‑1 (MQ=255)                                                                                                            cGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAg                                                                         >  1:943364/1‑82 (MQ=255)                                                                                                                         aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCTG‑CCAGCGTGcc                                                                                           <  2:789546/51‑1 (MQ=255)                                                                                                                         aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGcc                                                                                           >  1:789546/1‑51 (MQ=255)                                                                                                                         aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAg                                                                                 >  2:1192064/1‑61 (MQ=255)                                                                                                                         aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAg                                                                                 <  1:1192064/61‑1 (MQ=255)                                                                                                                         aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACg                                          <  2:1665273/100‑1 (MQ=255)                                                                                                                         aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACg                                          >  1:1665273/1‑100 (MQ=255)                                                                                                                         aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa   >  1:780883/1‑139 (MQ=255)                                                                                                                         aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa   >  2:42568/1‑139 (MQ=255)                                                                                                                         aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCa    >  1:1136946/1‑138 (MQ=255)                                                                                                                         aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCa    <  2:1136946/138‑1 (MQ=255)                                                                                                                          tCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaaa  <  2:441613/139‑1 (MQ=255)                                                                                                                           cAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCa                                                         >  2:1241094/1‑84 (MQ=255)                                                                                                                           cAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCa                                                         <  1:1241094/84‑1 (MQ=255)                                                                                                                              aaGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCa                                <  1:107429/105‑1 (MQ=255)                                                                                                                              aaGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCa                                >  2:107429/1‑105 (MQ=255)                                                                                                                              aaGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTa                          >  2:820713/1‑111 (MQ=255)                                                                                                                              aaGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTa                          <  1:820713/111‑1 (MQ=255)                                                                                                                                    |                                                                                                                                  GCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAA  >  NC_000913/1071929‑1072188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑