| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,757,721 | C→T | *335* (TAG→TAA) | ldtE ← | murein L,D‑transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,757,721 | 0 | C | T | 100.0% | 164.4 / NA | 51 | *335* (TAG→TAA) | ldtE | murein L,D‑transpeptidase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (27/24); total (27/24) | |||||||||||
TTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGC > NC_000913/1757584‑1757855 | ttCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGAGCACATTGTGCGCCA‑‑TTTTTTTGTCTGCCGTTTACCGTTa > 1:344292/1‑139 (MQ=255) cGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccatt > 2:976609‑M1/1‑131 (MQ=255) acaacaTGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct < 1:829268‑M1/139‑16 (MQ=255) aacaTGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctga < 1:976609‑M1/139‑18 (MQ=255) acaTGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgac > 1:1598507‑M1/1‑121 (MQ=255) ggCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct < 2:73680‑M1/132‑16 (MQ=255) ggCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct > 1:73680‑M1/1‑117 (MQ=255) aCCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGt > 1:1022522/1‑83 (MQ=255) aCCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGt < 2:1022522/83‑1 (MQ=255) aCCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct < 1:1295526‑M1/121‑16 (MQ=255) aCCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct > 2:1295526‑M1/1‑106 (MQ=255) cGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTc > 2:595684/1‑82 (MQ=255) cGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTc < 1:595684/82‑1 (MQ=255) aaGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgct > 1:106995‑M1/1‑101 (MQ=255) gTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgc > 2:1514436‑M1/1‑99 (MQ=255) gTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgc < 1:1514436‑M1/113‑15 (MQ=255) tACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcggg < 2:106995‑M1/139‑48 (MQ=255) tgtgAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgc > 2:85774‑M1/1‑88 (MQ=255) tgtgAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgc < 1:85774‑M1/102‑15 (MQ=255) gtgaagtgaaAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACat > 1:623727/1‑76 (MQ=255) gtgaagtgaaAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACat < 2:623727/76‑1 (MQ=255) tgaagtgaaAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgc > 2:1591134‑M1/1‑86 (MQ=255) tgaagtgaaAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATACCCTTGCTtcaccattctgcgc < 1:1203294‑M1/100‑15 (MQ=255) tgaagtgaaAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATACCCTTGCTtcaccattctgcgc > 2:1203294‑M1/1‑86 (MQ=255) gaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgct > 1:602370‑M1/1‑86 (MQ=255) gaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgct < 2:602370‑M1/124‑39 (MQ=255) aaaaaTGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactg < 2:1598507‑M1/139‑61 (MQ=255) aaaaaTGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactg > 2:224828‑M1/1‑79 (MQ=255) aaaaaTGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct < 2:1624426‑M1/94‑16 (MQ=255) aaaaaTGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct > 1:1624426‑M1/1‑79 (MQ=255) aaTGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgct > 1:1539129‑M1/1‑76 (MQ=255) aaTGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgct < 2:1539129‑M1/114‑39 (MQ=255) cgcACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTt > 2:490958‑M1/1‑71 (MQ=255) cgcACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTt < 1:490958‑M1/72‑2 (MQ=255) aTTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct < 1:1567248‑M1/81‑16 (MQ=255) aTTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct > 2:1567248‑M1/1‑66 (MQ=255) tgCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACa > 2:349963/1‑50 (MQ=255) tgCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACa < 1:349963/50‑1 (MQ=255) a‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggataccctgc > 1:1441622‑M1/1‑56 (MQ=255) tttttcttGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattttgcgctgactctactgaaggcgcagtgctggctgcgggagttgctccactgctccccgaaaccggataccctgcc < 1:1072204‑M1/139‑85 (MQ=255) cTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCt > 2:510652/1‑41 (MQ=255) cTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCt < 1:510652/41‑1 (MQ=255) cTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggataccctgcccgacgataca > 1:291909‑M1/1‑45 (MQ=255) cTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcg < 1:1228977‑M1/76‑32 (MQ=255) cTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcg > 2:1228977‑M1/1‑45 (MQ=255) cTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgc < 2:1350851‑M1/57‑13 (MQ=255) cTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgc > 1:1350851‑M1/1‑45 (MQ=255) gCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcg > 2:276665‑M1/1‑43 (MQ=255) gCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcg < 1:276665‑M1/74‑32 (MQ=255) tttACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggataccctgcccgacgatacaacgctt > 2:137409‑M1/1‑39 (MQ=255) aCCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggataccctgcccgacgatacaacgctttat < 1:137409‑M1/139‑104 (MQ=255) | TTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGC > NC_000913/1757584‑1757855 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |