Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	183,620	G→A	*386* (TAG→TAA)	cdaR →	carbohydrate diacid regulon transcriptional regulator, autoregulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	183,620	0	G	A	100.0%	182.8 / NA	57	*386* (TAG→TAA)	cdaR	carbohydrate diacid regulon transcriptional regulator, autoregulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/25);  total (32/25)

TTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCT  >  NC_000913/183492‑183755                                                                                                                                  |                                                                                                                                        ttgttTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTa                                                                                                                              >  2:759230/1‑139 (MQ=255)              gTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTG‑‑TGGAGAGGGGGAAGTTATTCGGTTAGGTTTAgggggtg                                                                                                                >  1:754157/1‑137 (MQ=255)               tAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                                <  1:759230/139‑1 (MQ=255)                       tGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCa                                                                                                        >  1:881242/1‑139 (MQ=255)                       tGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCa                                                                                                        >  2:764644/1‑139 (MQ=255)                            tATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTccc                                                                                                   >  1:636977/1‑139 (MQ=255)                            tATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTccc                                                                                                   >  1:86175/1‑139 (MQ=255)                            tATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTccc                                                                                                   <  2:86175/139‑1 (MQ=255)                              tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                                 >  1:900949/1‑139 (MQ=255)                               ccggttaaatgTAAATTGGAACTGGGCCGGGTTGGTTTGGGGAATTTTGGATGACAGG‑TGGTTCTTTATGTTGCCTTAAAACTTG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTc                                                                                                <  2:754157/129‑1 (MQ=255)                               cGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTc                                                                                                >  2:527674/1‑139 (MQ=255)                               cGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTc                                                                                                <  2:636977/139‑1 (MQ=255)                               cGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTc                                                                                                >  1:203083/1‑139 (MQ=255)                                 gCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAg                                                                                              >  2:353683/1‑139 (MQ=255)                                     aaTCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                                          <  2:881242/139‑1 (MQ=255)                                      aTCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGa                                                                                         >  1:266943/1‑139 (MQ=255)                                       tCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                        <  1:764644/139‑1 (MQ=255)                                         gTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACACGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAgg                                                                                      <  2:900949/139‑1 (MQ=255)                                            tatCGGAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGCGTa                                                                                   >  2:1085099/1‑139 (MQ=255)                                                 gAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGC‑TTACAACTGG‑TGAAGAGCGGTAAGTTATGCGtt                                                                                                                               >  2:1063876/1‑89 (MQ=255)                                                 gAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGC‑TTACAACTGG‑TGAAGAGCGGTAAGTTATGCGtt                                                                                                                               <  1:1063876/89‑1 (MQ=255)                                                    cTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGc                                                                                             >  1:1227214/1‑121 (MQ=255)                                                    cTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGc                                                                                             <  2:1227214/121‑1 (MQ=255)                                                    cTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGaa                                                                           <  1:1085099/139‑1 (MQ=255)                                                      gACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATg                                                                         >  1:253691/1‑139 (MQ=255)                                                       aCCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                        <  1:1270882/123‑1 (MQ=255)                                                       aCCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                        >  2:1270882/1‑123 (MQ=255)                                                            gCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCa                                                                                                        >  1:1085799/1‑102 (MQ=255)                                                            gCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCa                                                                                                        <  2:1085799/102‑1 (MQ=255)                                                            gCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                                          <  1:462185/116‑1 (MQ=255)                                                            gCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                                          >  2:462185/1‑116 (MQ=255)                                                            gCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGaa                                                                   >  1:148704/1‑139 (MQ=255)                                                                    tGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGt                                                           <  1:353683/139‑1 (MQ=255)                                                                    tGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGt                                                           <  2:148704/139‑1 (MQ=255)                                                                      ggCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTGTGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATCAACCGTCa                                                         >  2:994019/1‑139 (MQ=255)                                                                      ggCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCa                                                         >  1:1581681/1‑139 (MQ=255)                                                                                       ggTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTAc                                         >  1:318888/1‑139 (MQ=255)                                                                                          tgctgcTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTAcgcg                                      <  1:527674/139‑1 (MQ=255)                                                                                          tgctgcTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTAcgcg                                      <  1:994019/139‑1 (MQ=255)                                                                                                  tATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGtttt                              >  2:24041/1‑139 (MQ=255)                                                                                                    tgtgGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTc                            >  1:1496411/1‑139 (MQ=255)                                                                                                    tgtgGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTc                            >  1:330112/1‑139 (MQ=255)                                                                                                         cGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGt                                                           >  1:1138600/1‑103 (MQ=255)                                                                                                         cGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGt                                                           <  2:1138600/103‑1 (MQ=255)                                                                                                         cGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCggg                                                  >  1:775704/1‑112 (MQ=255)                                                                                                         cGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCggg                                                  <  2:775704/112‑1 (MQ=255)                                                                                                            tACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCgg                    >  2:95982/1‑139 (MQ=255)                                                                                                              cAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCt                  <  2:1496411/139‑1 (MQ=255)                                                                                                               aaCTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCtt                 <  2:330112/139‑1 (MQ=255)                                                                                                                          gagCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                                          >  1:881240/1‑56 (MQ=255)                                                                                                                          gagCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                                          <  2:881240/56‑1 (MQ=255)                                                                                                                          gagCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCt       <  2:592483/139‑1 (MQ=255)                                                                                                                          gagCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCt       <  1:262358/139‑1 (MQ=255)                                                                                                                             cGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGAt    <  1:1152492/139‑1 (MQ=255)                                                                                                                             cGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGAt    >  2:1152492/1‑139 (MQ=255)                                                                                                                               gTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGc        >  2:1570334/1‑133 (MQ=255)                                                                                                                               gTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCt  >  2:1031068/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                        TTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTT‑GATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCT  >  NC_000913/183492‑183755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑