Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,964,697	G→A	L118L (CTC→CTT)	flhA ←	putative flagellar export pore protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,964,697	0	G	A	100.0%	247.3 / NA	77	L118L (CTC→CTT)	flhA	putative flagellar export pore protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/37);  total (40/37)

CTTACCCGGCATACCATCGAGAACAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACGAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGAA  >  NC_000913/1964562‑1964821                                                                                                                                        |                                                                                                                              cTTACCCGGCATACCATCGAGAACAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAgg                                                                                                                            >  1:566400/1‑139 (MQ=255)     cccGGCATACCATCGAGAACAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGt                                                                                                                        >  1:980802/1‑139 (MQ=255)           aTACCATCGAGAACAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATTACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGa                                                                                                                  <  2:1128445/139‑1 (MQ=255)                    agaACAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGa                                                                                                         >  1:1059026/1‑139 (MQ=255)                    agaACAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGa                                                                                                         <  2:1059026/139‑1 (MQ=255)                    agaACAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGa                                                                                                         >  2:907029/1‑139 (MQ=255)                     gaACAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGa‑c                                                                                                       <  2:968172/139‑1 (MQ=255)                        cAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGa‑ccac                                                                                                    >  2:185368/1‑139 (MQ=255)                         aaaGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACTAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGa‑cc                                                                                                      <  2:946617/136‑1 (MQ=255)                         aaaGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGa‑cc                                                                                                      >  1:946617/1‑136 (MQ=255)                           aGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCg                                                                                                          <  1:866109/131‑1 (MQ=255)                           aGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCg                                                                                                          >  2:866109/1‑131 (MQ=255)                           aGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCtt                                                                                                 >  1:105031/1‑139 (MQ=255)                                          cTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCcgccgccgc                                                                                  <  1:1517235/139‑1 (MQ=255)                                           tGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCcgccgcg                                                                                 <  2:980802/139‑1 (MQ=255)                                           tGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCcgccgcg                                                                                 <  2:105031/139‑1 (MQ=255)                                                  cGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCcgccgcg                                                                                 >  1:84609/1‑132 (MQ=255)                                                  cGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCcgccgcg                                                                                 <  2:84609/132‑1 (MQ=255)                                                  cGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTa                                                                           >  2:1476838/1‑138 (MQ=255)                                                  cGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTa                                                                           <  1:1476838/138‑1 (MQ=255)                                                  cGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTAt                                                                          >  2:816331/1‑139 (MQ=255)                                                  cGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTAt                                                                          >  1:634258/1‑139 (MQ=255)                                                  cGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTAt                                                                          >  1:1190073/1‑139 (MQ=255)                                                    cccGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAAc                                                                                                                >  2:244475/1‑100 (MQ=255)                                                    cccGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAAc                                                                                                                <  1:244475/100‑1 (MQ=255)                                                      cGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGccc                                                                      <  1:816331/139‑1 (MQ=255)                                                       ggCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCt                                                                     <  2:566400/139‑1 (MQ=255)                                                             tttGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCtt                                                                    <  2:1261879/134‑1 (MQ=255)                                                             tttGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCtt                                                                    >  1:1261879/1‑134 (MQ=255)                                                                ggTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTaaa                                                            <  2:1327350/139‑1 (MQ=255)                                                                      gACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACg                                                                                                               >  1:1488237/1‑83 (MQ=255)                                                                      gACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACg                                                                                                               <  2:1488237/83‑1 (MQ=255)                                                                      gACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATa                                                      <  2:1190073/139‑1 (MQ=255)                                                                      gACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATa                                                      <  2:1652603/139‑1 (MQ=255)                                                                      gACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTCCCATTAAAATGATa                                                      >  1:1052384/1‑139 (MQ=255)                                                                      gACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCAACAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATa                                                      >  1:1565271/1‑139 (MQ=255)                                                                           tAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGt                                                 >  2:1501929/1‑139 (MQ=255)                                                                               gTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGaa                                             <  2:1565271/139‑1 (MQ=255)                                                                                 tgatgaTCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTCCCATTAAAATGATACGGGTTGAAGc                                           <  2:1052384/139‑1 (MQ=255)                                                                                  gatgatCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGcc                                          >  1:1645346/1‑139 (MQ=255)                                                                                   atgatCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGa                                                                                                                  >  1:1408419/1‑67 (MQ=255)                                                                                   atgatCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGa                                                                                                                  <  2:1408419/67‑1 (MQ=255)                                                                                   atgatCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCAt                                                                >  2:986268/1‑116 (MQ=255)                                                                                   atgatCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGtt                                                <  2:1643632/132‑1 (MQ=255)                                                                                   atgatCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGtt                                                >  1:1643632/1‑132 (MQ=255)                                                                                   atgatCACGAGAATGACAAACACCACGATACCGAAAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCAt                                                                <  1:986268/116‑1 (MQ=255)                                                                                        cACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGACCCACCTTCCCTGCCGCcgccgcg                                                                                 >  1:1537629/1‑95 (MQ=255)                                                                                        cACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGACCCACCTTCCCTGCCGCcgccgcg                                                                                 <  2:1537629/95‑1 (MQ=255)                                                                                          cGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAg                                  >  1:1573016/1‑139 (MQ=255)                                                                                            agaATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTg                                <  2:1645346/139‑1 (MQ=255)                                                                                             gaATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATa                                                      <  2:512140/116‑1 (MQ=255)                                                                                             gaATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATa                                                      >  1:512140/1‑116 (MQ=255)                                                                                                tGACAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAg                            >  2:1540253/1‑139 (MQ=255)                                                                                                  aCAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGAc                          >  1:1409214/1‑139 (MQ=255)                                                                                                  aCAAACACCACGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGAc                          >  1:128865/1‑139 (MQ=255)                                                                                                        accacGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACa                    <  1:907029/139‑1 (MQ=255)                                                                                                         ccacGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCcgccgcg                                                                                 >  2:1671465/1‑77 (MQ=255)                                                                                                         ccacGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCcgccgcg                                                                                 <  1:1671465/77‑1 (MQ=255)                                                                                                            cGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAAc                                                                                                                >  2:17001/1‑44 (MQ=255)                                                                                                            cGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAAc                                                                                                                <  1:17001/44‑1 (MQ=255)                                                                                                            cGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGt                >  1:1394595/1‑139 (MQ=255)                                                                                                            cGATACCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGt                >  1:46763/1‑139 (MQ=255)                                                                                                                aCCGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTa            <  2:1573016/139‑1 (MQ=255)                                                                                                                  cGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCaa                      <  2:709910/127‑1 (MQ=255)                                                                                                                  cGATAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCaa                      >  1:709910/1‑127 (MQ=255)                                                                                                                    aTAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGccc                                                                      <  1:891958/77‑1 (MQ=255)                                                                                                                    aTAGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGccc                                                                      >  2:891958/1‑77 (MQ=255)                                                                                                                      aGCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAaacaac      >  2:556652/1‑139 (MQ=255)                                                                                                                       gCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCcgccgcg                                                                                 >  1:942246/1‑63 (MQ=255)                                                                                                                       gCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCcgccgcg                                                                                 <  2:942246/63‑1 (MQ=255)                                                                                                                       gCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAg                                            >  2:1663373/1‑100 (MQ=255)                                                                                                                       gCGAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAg                                            <  1:1663373/100‑1 (MQ=255)                                                                                                                         gAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGa   >  1:216716/1‑139 (MQ=255)                                                                                                                         gAAATTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGa   <  1:185368/139‑1 (MQ=255)                                                                                                                          aaaTTGCCACCAACAAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGaa  <  2:634258/139‑1 (MQ=255)                                                                                                                                ccaccaACAAGGAAGTGACCGAACGCTTCGA‑CCACCTT‑CCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGaa  <  2:1224397/132‑1 (MQ=255)                                                                                                                                ccaccaACAAGGAAGTGACCGAACGCTTCGA‑CCACCTT‑CCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGaa  >  1:1224397/1‑132 (MQ=255)                                                                                                                                        |                                                                                                                              CTTACCCGGCATACCATCGAGAACAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACGAGGAAGTGACCGAACGCTTCGA‑CCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGAA  >  NC_000913/1964562‑1964821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑