Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	197,574	T→C	S343S (AGT→AGC)	rseP →	inner membrane zinc RIP metalloprotease, RpoE activator, by degrading RseA, cleaved signal peptide endoprotease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	197,574	0	T	C	100.0%	236.6 / NA	66	S343S (AGT→AGC)	rseP	inner membrane zinc RIP metalloprotease, RpoE activator, by degrading RseA, cleaved signal peptide endoprotease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/34);  total (32/34)

ATTGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGTATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTG  >  NC_000913/197440‑197709                                                                                                                                        |                                                                                                                                        aTTGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGc                                                                                                                                     <  2:1641788/139‑1 (MQ=255) aTTGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGc                                                                                                                                     >  1:424917/1‑139 (MQ=255)  ttGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCt                                                                                                                                    <  1:1503862/139‑1 (MQ=255)       tttGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGaa                                                                                                                               <  1:1220139/139‑1 (MQ=255)        ttGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGaaa                                                                                                                              >  2:425976/1‑139 (MQ=255)         tGTCGGTATTGAGCCGAAAGCCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGaaaa                                                                                                                             >  2:1592817/1‑139 (MQ=255)                    aGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCgg                                                                                                                  >  1:1261538/1‑139 (MQ=255)                    aGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCgg                                                                                                                  >  1:159365/1‑139 (MQ=255)                     gCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGc                                                                                                                                     <  2:676806/119‑1 (MQ=255)                     gCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGc                                                                                                                                     >  1:676806/1‑119 (MQ=255)                         aaaGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGAtg                                                                                                             >  2:624712/1‑139 (MQ=255)                         aaaGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGAtg                                                                                                             <  2:159365/139‑1 (MQ=255)                           aGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCGGATGAAGCTGACGGTCAGCATGCTGGGAAAATTTATCACCGGTGAtgtg                                                                                                           >  2:342714/1‑139 (MQ=255)                                       gCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaacaac                                                                                               <  2:424917/139‑1 (MQ=255)                                            aTGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAg                                                                                          >  2:1624669/1‑139 (MQ=255)                                             tGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATAGATCACCGGTGATGTGAAACTGAACAACCTCAGt                                                                                         <  1:896538/139‑1 (MQ=255)                                                 tataAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCgg                                                                                                                  <  2:549175/110‑1 (MQ=255)                                                 tataAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCgg                                                                                                                  >  1:549175/1‑110 (MQ=255)                                                     aaGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGAt                                                                                 <  1:624712/139‑1 (MQ=255)                                                           tACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGAtg                                                                                                             <  2:611840/105‑1 (MQ=255)                                                           tACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGAtg                                                                                                             >  1:611840/1‑105 (MQ=255)                                                           tACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTAt                                                                           >  1:1484594/1‑139 (MQ=255)                                                           tACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTAt                                                                           >  1:456825/1‑139 (MQ=255)                                                            aCGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATc                                                                          <  1:425976/139‑1 (MQ=255)                                                                 aGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCaa                                                                     <  2:43358/139‑1 (MQ=255)                                                                   tATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAgg                                                                   <  2:1195970/139‑1 (MQ=255)                                                                       ggCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGc                                                               <  2:456825/139‑1 (MQ=255)                                                                        gCCGTTCAACGCCATTATCCAACCCTC‑GGACCAAACGTGGCCGCTGATGCAGCTGCCCCTCAGCATGTTGGGAAAATTGATCACCGGTCATGTGAAACTGAACAACCTCAGTCGGCCGATCTCTATCGCCAAGGGGGCt                                                              <  1:342714/139‑1 (MQ=255)                                                                        gCCGTTCAACGCCATCGTCGAAGCCACGGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGaaaa                                                                                                                             >  1:454492/1‑77 (MQ=255)                                                                        gCCGTTCAACGCCATCGTCGAAGCCACGGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGaaaa                                                                                                                             <  2:454492/77‑1 (MQ=255)                                                                        gCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAg                                                                    >  1:701656/1‑133 (MQ=255)                                                                        gCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAg                                                                    <  2:701656/133‑1 (MQ=255)                                                                            ttCAACGCCTTCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaa                                                                                                   >  1:61493/1‑98 (MQ=255)                                                                            ttCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTgg                                                                                                                                  >  2:1579934/1‑67 (MQ=255)                                                                            ttCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTgg                                                                                                                                  <  1:1579934/67‑1 (MQ=255)                                                                            ttCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaa                                                                                                   <  2:61493/98‑1 (MQ=255)                                                                            ttCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTggg                                                                                      >  1:72209/1‑111 (MQ=255)                                                                            ttCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTggg                                                                                      <  2:72209/111‑1 (MQ=255)                                                                            ttCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGa                                                          >  2:310917/1‑139 (MQ=255)                                                                            ttCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGa                                                          >  1:94811/1‑139 (MQ=255)                                                                            ttCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGa                                                          >  2:1346668/1‑139 (MQ=255)                                                                            ttCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGa                                                          <  1:1523886/139‑1 (MQ=255)                                                                            ttCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGa                                                          >  1:855227/1‑139 (MQ=255)                                                                             tCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCt                                                                                                                                    >  1:446449/1‑64 (MQ=255)                                                                             tCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCt                                                                                                                                    <  2:446449/64‑1 (MQ=255)                                                                             tCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCaa                                                                     >  2:761935/1‑127 (MQ=255)                                                                             tCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCaa                                                                     <  1:761935/127‑1 (MQ=255)                                                                                              gCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                                        <  2:1484594/139‑1 (MQ=255)                                                                                              gCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                                        <  1:1624669/139‑1 (MQ=255)                                                                                                  c‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGgttgg                                    >  1:1207509/1‑138 (MQ=255)                                                                                                  c‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGgt                                       >  1:1131976/1‑136 (MQ=255)                                                                                                  c‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGgt                                       <  2:1131976/136‑1 (MQ=255)                                                                                                    ggACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                                        <  1:553314/134‑1 (MQ=255)                                                                                                    ggACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                                        >  1:1066669/1‑134 (MQ=255)                                                                                                    ggACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                                        <  2:1066669/134‑1 (MQ=255)                                                                                                    ggACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                                        >  2:553314/1‑134 (MQ=255)                                                                                                               ggCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                                        <  1:1543865/123‑1 (MQ=255)                                                                                                               ggCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                                        >  2:1543865/1‑123 (MQ=255)                                                                                                               ggCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGcc                        >  1:1336949/1‑139 (MQ=255)                                                                                                                gCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGcc                        >  2:12147/1‑138 (MQ=255)                                                                                                                gCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGcc                        <  1:12147/138‑1 (MQ=255)                                                                                                                    cTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTc                   <  2:1261538/139‑1 (MQ=255)                                                                                                                      gatgaAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTTCCTGCCGTTTCtt                 <  1:1592817/139‑1 (MQ=255)                                                                                                                             cTGACGGTCAGCATGCTGGGAAAATTGATCACCgg                                                                                                                  >  2:986936/1‑35 (MQ=255)                                                                                                                             cTGACGGTCAGCATGCTGGGAAAATTGATCACCgg                                                                                                                  <  1:986936/35‑1 (MQ=255)                                                                                                                                     cAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTg  <  2:1327058/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        ATTGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCAC‑GGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGTATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGTG  >  NC_000913/197440‑197709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑