Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,976,605	T→C	F110L (TTT→CTT)	wzxE →	O‑antigen translocase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,976,605	0	T	C	100.0%	197.6 / NA	55	F110L (TTT→CTT)	wzxE	O‑antigen translocase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/28);  total (27/28)

TACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTT  >  NC_000913/3976470‑3976726                                                                                                                                        |                                                                                                                           tACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTg                                                                                                                         >  1:1343783/1‑139 (MQ=255)           aGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGa                                                                                                               <  2:1369919/139‑1 (MQ=255)                cATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATc                                                                                                          >  2:1258611/1‑139 (MQ=255)                  tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGa                                                                                                               <  2:642334/132‑1 (MQ=255)                  tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGa                                                                                                               >  1:642334/1‑132 (MQ=255)                  tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATc                                                                                                          <  2:657276/137‑1 (MQ=255)                  tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATc                                                                                                          >  1:657276/1‑137 (MQ=255)                  tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑g                                                                                                       >  2:302672/1‑139 (MQ=255)                     tAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑gggg                                                                                                    <  2:450037/139‑1 (MQ=255)                       aTCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGc                                                                                                   <  2:1413029/138‑1 (MQ=255)                       aTCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGc                                                                                                   >  1:1413029/1‑138 (MQ=255)                          cGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGt                                                                                               <  2:1343783/139‑1 (MQ=255)                                             ggTCGGCACTTCATCATCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTCGTg                                                                            <  2:1136466/139‑1 (MQ=255)                                                      ttCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATgggg                                                                   <  1:302672/139‑1 (MQ=255)                                                       tcatcaGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGa                                                                  <  1:1258611/139‑1 (MQ=255)                                                        catcaGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGAt                                                                 >  1:897078/1‑139 (MQ=255)                                                         atcaGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATc                                                                >  1:1422777/1‑139 (MQ=255)                                                         atcaGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATc                                                                >  2:955834/1‑139 (MQ=255)                                                                aTGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTg                                                                            >  2:80120/1‑120 (MQ=255)                                                                aTGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTg                                                                            <  1:80120/120‑1 (MQ=255)                                                                aTGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTggg                                                         >  2:1643075/1‑139 (MQ=255)                                                                 tGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTgggg                                                        <  2:1422777/139‑1 (MQ=255)                                                                  ggTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGACTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAATTGGGGATCGCCTGGGGc                                                       <  1:955834/139‑1 (MQ=255)                                                                    tACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTg                                                                            >  2:682822/1‑116 (MQ=255)                                                                    tACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTg                                                                            <  1:682822/116‑1 (MQ=255)                                                                    tACTTGGTTTCTCTACGCTGATGGCGCTGGGTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTACCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCaa                                                     >  1:103716/1‑139 (MQ=255)                                                                     aCTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAAc                                                    <  2:201217/139‑1 (MQ=255)                                                                           tttCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGGCTGGTGCGTTTAGTGGCGCTGGTg                                                                            >  2:1413467/1‑110 (MQ=255)                                                                           tttCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGGCTGGTGCGTTTAGTGGCGCTGGTg                                                                            <  1:1413467/110‑1 (MQ=255)                                                                           tttCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGt                                                                                               >  2:703699/1‑90 (MQ=255)                                                                           tttCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGt                                                                                               <  1:703699/90‑1 (MQ=255)                                                                              ctctACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGa                                                                                                               <  2:1609666/72‑1 (MQ=255)                                                                              ctctACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGa                                                                                                               >  1:1609666/1‑72 (MQ=255)                                                                              ctctACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCaaa                                                                        >  1:977216/1‑110 (MQ=255)                                                                              ctctACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCaaa                                                                        <  2:977216/110‑1 (MQ=255)                                                                                        aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCt                                                            <  2:257773/112‑1 (MQ=255)                                                                                        aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCt                                                            >  1:257773/1‑112 (MQ=255)                                                                                        aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCtgatga                                 <  2:897078/139‑1 (MQ=255)                                                                                         tGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGaa                                >  1:1195166/1‑139 (MQ=255)                                                                                              cTGGTTTTTGTGTTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑gggg                                                                                                    >  1:914478/1‑66 (MQ=255)                                                                                              cTGGTTTTTGTGTTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑gggg                                                                                                    <  2:914478/66‑1 (MQ=255)                                                                                                      tgtgCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGAt                   <  2:411861/139‑1 (MQ=255)                                                                                                          cTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCg               <  2:1195166/139‑1 (MQ=255)                                                                                                           tGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGt                                                                                      <  1:515714/67‑1 (MQ=255)                                                                                                           tGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGt                                                                                      >  2:515714/1‑67 (MQ=255)                                                                                                           tGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTgggg                                                        >  2:981063/1‑97 (MQ=255)                                                                                                           tGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTgggg                                                        <  1:981063/97‑1 (MQ=255)                                                                                                            ggCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCa             >  2:1310270/1‑139 (MQ=255)                                                                                                                  tGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTcgc                      >  2:1601952/1‑124 (MQ=255)                                                                                                                  tGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTcgc                      <  1:1601952/124‑1 (MQ=255)                                                                                                                       cAATCAGCCAGGGATTGCTTGGTAACACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGtt  >  1:775545/1‑139 (MQ=255)                                                                                                                         aTCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCt                                      <  2:1230383/101‑1 (MQ=255)                                                                                                                         aTCAGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCt                                      >  1:1230383/1‑101 (MQ=255)                                                                                                                            aGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGc                                                       >  2:420307/1‑81 (MQ=255)                                                                                                                            aGCCAGGGATTGCTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGc                                                       <  1:420307/81‑1 (MQ=255)                                                                                                                                        |                                                                                                                           TACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCA‑GGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTT  >  NC_000913/3976470‑3976726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑