Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,117,123	G→A	R34C (CGC→TGC)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,123	0	G	A	100.0%	288.7 / NA	88	R34C (CGC→TGC)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/45);  total (43/45)

CCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTA  >  NC_000913/4116987‑4117247                                                                                                                                          |                                                                                                                             ccGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACt                                                                                                                            >  1:122546/1‑139 (MQ=255)    aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCg                                                                                                                         >  2:278701/1‑139 (MQ=255)    aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCg                                                                                                                         >  1:18848/1‑139 (MQ=255)    aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCg                                                                                                                         >  2:1318483/1‑139 (MQ=255)     tAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                        <  1:278701/139‑1 (MQ=255)     tAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                        <  2:18848/139‑1 (MQ=255)      aGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGac                                                                                                                       <  2:1524803/139‑1 (MQ=255)      aGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGac                                                                                                                       <  1:756935/139‑1 (MQ=255)        cTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGaca                                                                                                                      <  1:582438/139‑1 (MQ=255)         tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGaca                                                                                                                      >  1:1465931/1‑137 (MQ=255)         tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGaca                                                                                                                      <  2:1465931/137‑1 (MQ=255)         tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACg                                                                                                                    >  2:684623/1‑139 (MQ=255)         tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACg                                                                                                                    >  1:1650516/1‑139 (MQ=255)         tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACg                                                                                                                    >  2:337255/1‑139 (MQ=255)         tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACg                                                                                                                    >  2:814261/1‑139 (MQ=255)         tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACg                                                                                                                    <  1:1318483/139‑1 (MQ=255)           cAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCt                                                                                                                  >  1:1599121/1‑139 (MQ=255)            aaTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTa                                                                                                                 <  1:814261/139‑1 (MQ=255)            aaTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTa                                                                                                                 <  2:1650516/139‑1 (MQ=255)                  aTCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGata                                                                                                           <  1:337255/139‑1 (MQ=255)                   tCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGatat                                                                                                          >  1:624908/1‑139 (MQ=255)                     ggAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTg                                                                                                        <  2:624908/139‑1 (MQ=255)                       aaCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGc                                                                                                                          <  1:156140/119‑1 (MQ=255)                       aaCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGc                                                                                                                          >  2:156140/1‑119 (MQ=255)                          tGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATc                                                                                                   <  2:1599121/139‑1 (MQ=255)                           gATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCg                                                                                                  <  2:634653/139‑1 (MQ=255)                                      tCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGaca                                                                                                                      >  1:331825/1‑108 (MQ=255)                                      tCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGaca                                                                                                                      <  2:331825/108‑1 (MQ=255)                                      tCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTa                                                                                                                 <  2:1470541/113‑1 (MQ=255)                                      tCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTa                                                                                                                 >  1:1470541/1‑113 (MQ=255)                                      tCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTAc                                                                                       >  1:246520/1‑139 (MQ=255)                                      tCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTAc                                                                                       >  1:1370655/1‑139 (MQ=255)                                                 tCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCggg                                                                            <  2:246520/139‑1 (MQ=255)                                                  cTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGac                                                                                                                       <  2:1344759/95‑1 (MQ=255)                                                  cTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGac                                                                                                                       >  1:1344759/1‑95 (MQ=255)                                                    aCCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                                                         <  2:122546/139‑1 (MQ=255)                                                        gCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggt                                                                     >  1:1540316/1‑139 (MQ=255)                                                         cGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTa                                                                                                                 <  2:1415352/94‑1 (MQ=255)                                                         cGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTa                                                                                                                 <  2:1479789/94‑1 (MQ=255)                                                         cGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTa                                                                                                                 >  1:1479789/1‑94 (MQ=255)                                                         cGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTa                                                                                                                 >  1:1415352/1‑94 (MQ=255)                                                          gTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTg                                                                                                        <  2:243710/102‑1 (MQ=255)                                                          gTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTg                                                                                                        >  1:243710/1‑102 (MQ=255)                                                          gTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                               <  1:1428662/111‑1 (MQ=255)                                                          gTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                               >  2:1428662/1‑111 (MQ=255)                                                          gTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                                                                   >  1:666897/1‑139 (MQ=255)                                                             gAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                                                         >  1:821458/1‑130 (MQ=255)                                                             gAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                                                         <  2:821458/130‑1 (MQ=255)                                                              aGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                        <  1:677773/82‑1 (MQ=255)                                                              aGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                        >  2:677773/1‑82 (MQ=255)                                                                   tGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggt                                                                     >  1:354318/1‑128 (MQ=255)                                                                   tGGGTGGCCCAGATTTCCAGTGGGTCGTTTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggt                                                                     <  2:354318/128‑1 (MQ=255)                                                                      gTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGAc                                                                                    >  2:416812/1‑110 (MQ=255)                                                                      gTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGAc                                                                                    <  1:416812/110‑1 (MQ=255)                                                                         gCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCa                                                                                                     >  2:933784/1‑90 (MQ=255)                                                                         gCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCa                                                                                                     <  1:933784/90‑1 (MQ=255)                                                                         gCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAgcg                                                    <  1:684623/139‑1 (MQ=255)                                                                          cccAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                                                                   >  1:930425/1‑123 (MQ=255)                                                                          cccAGATTACCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                                                                   <  2:930425/123‑1 (MQ=255)                                                                            cAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGccc                                                              >  1:1049656/1‑126 (MQ=255)                                                                            cAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGccc                                                              <  2:1049656/126‑1 (MQ=255)                                                                            cAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCaa                                                 <  2:666897/139‑1 (MQ=255)                                                                                    cATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                        >  2:541216/1‑60 (MQ=255)                                                                                    cATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                        <  1:541216/60‑1 (MQ=255)                                                                                         ggTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGaca                                                                                                                      >  1:1598318/1‑57 (MQ=255)                                                                                         ggTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGaca                                                                                                                      <  2:1598318/57‑1 (MQ=255)                                                                                         ggTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                        <  1:487903/55‑1 (MQ=255)                                                                                         ggTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                        >  2:487903/1‑55 (MQ=255)                                                                                         ggTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                               <  2:1585781/80‑1 (MQ=255)                                                                                         ggTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                               >  1:1585781/1‑80 (MQ=255)                                                                                         ggTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg                                                                    >  2:190569/1‑107 (MQ=255)                                                                                         ggTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg                                                                    <  1:190569/107‑1 (MQ=255)                                                                                              tgtTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGaca                                                                                                                      >  2:1307494/1‑52 (MQ=255)                                                                                              tgtTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGaca                                                                                                                      <  1:1307494/52‑1 (MQ=255)                                                                                              tgtTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGac                                                                                                                       <  1:51137/51‑1 (MQ=255)                                                                                              tgtTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGac                                                                                                                       >  2:51137/1‑51 (MQ=255)                                                                                              tgtTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAg                               >  1:1149341/1‑139 (MQ=255)                                                                                              tgtTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAg                               <  2:1370655/139‑1 (MQ=255)                                                                                               gtTCTA‑CCCTACCTGGTTTTGGGTAGATTTGCTCGAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGt                              <  2:1072061/139‑1 (MQ=255)                                                                                                      cccAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggt                                                                     >  2:738786/1‑94 (MQ=255)                                                                                                      cccAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggt                                                                     <  1:738786/94‑1 (MQ=255)                                                                                                           ccTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg                                                                    <  2:222017/90‑1 (MQ=255)                                                                                                           ccTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg                                                                    >  1:222017/1‑90 (MQ=255)                                                                                                           ccTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAAtt                   >  2:220819/1‑139 (MQ=255)                                                                                                           ccTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAAtt                   <  2:1540316/139‑1 (MQ=255)                                                                                                                     ggTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtttttt                                    <  2:1634149/112‑1 (MQ=255)                                                                                                                     ggTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtttttt                                    >  1:1634149/1‑112 (MQ=255)                                                                                                                            ttGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTa  >  2:573049/1‑139 (MQ=255)                                                                                                                                          |                                                                                                                             CCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTA  >  NC_000913/4116987‑4117247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑