Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,685,542	(C)6→5	coding (1047/1404 nt)	fumC ←	fumarate hydratase (fumarase C),aerobic Class II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,685,537	0	C	.	100.0%	131.0 / NA	32	coding (1052/1404 nt)	fumC	fumarate hydratase (fumarase C),aerobic Class II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (15/17);  total (15/17)

TCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCA  >  NC_000913/1685402‑1685671                                                                                                                                        |                                                                                                                                       tCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGcccc                                                                                                                                     >  1:352979/1‑139 (MQ=255)   aCGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGccccca                                                                                                                                   <  1:20342/139‑2 (MQ=255)                    ccACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCAcgtcg                                                                                                                 <  2:835524/139‑1 (MQ=255)                        tGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTcc                                                                                                             >  1:1219961/1‑139 (MQ=255)                          cgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTcccc                                                                                                           >  1:579818/1‑139 (MQ=255)                           gcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCa                                                                                                          >  2:55037/1‑139 (MQ=255)                            cAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCAt                                                                                                         <  1:1111298/139‑1 (MQ=255)                             aGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATc                                                                                                        >  1:1154378/1‑139 (MQ=255)                                      tAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                               <  2:878577/139‑1 (MQ=255)                                          aCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCaga                                                                                           <  1:55037/139‑1 (MQ=255)                                              tCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCAcgtcgt                                                                                                                <  2:345355/114‑1 (MQ=255)                                              tCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCAcgtcgt                                                                                                                >  1:345355/1‑114 (MQ=255)                                                   gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                  <  2:1219961/139‑1 (MQ=255)                                                        cTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTccc                                                                                                            <  2:596133/108‑1 (MQ=255)                                                        cTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTccc                                                                                                            >  1:596133/1‑108 (MQ=255)                                                         tGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCt                                                                            <  2:579818/139‑1 (MQ=255)                                                             aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                        <  2:352979/139‑1 (MQ=255)                                                                                      tgtgGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGc                                               <  2:68307/139‑1 (MQ=255)                                                                                             cACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGc                                                                              >  1:440065/1‑101 (MQ=255)                                                                                             cACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGc                                                                              <  2:440065/101‑1 (MQ=255)                                                                                                  ttGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTg                                   >  2:860070/1‑139 (MQ=255)                                                                                                       cGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCgg                              >  2:257441/1‑139 (MQ=255)                                                                                                         gAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCt                            <  2:1154378/139‑1 (MQ=255)                                                                                                          aaGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTccc                                                                                                            <  2:1159867/58‑1 (MQ=255)                                                                                                          aaGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTccc                                                                                                            >  1:1159867/1‑58 (MQ=255)                                                                                                          aaGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcac                                                                         >  2:321407/1‑93 (MQ=255)                                                                                                          aaGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcac                                                                         <  1:321407/93‑1 (MQ=255)                                                                                                          aaGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                           >  2:370933/1‑139 (MQ=255)                                                                                                          aaGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                           >  1:409412/1‑139 (MQ=255)                                                                                                          aaGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                           >  1:1147400/1‑139 (MQ=255)                                                                                                           aGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCa                          <  2:1147400/139‑1 (MQ=255)                                                                                                                                   aaGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTgg                                                               >  2:419854/1‑78 (MQ=255)                                                                                                                                   aaGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTgg                                                               <  1:419854/78‑1 (MQ=255)                                                                                                                                   aaGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCa  <  1:860070/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCA  >  NC_000913/1685402‑1685671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑