| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,185,875 | (G)7→8 | intergenic (‑79/‑42) | rcnR ← / → rcnA | transcriptional repressor of rcnA/membrane protein conferring nickel and cobalt resistance |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,185,868 | 1 | . | G | 80.0% | 45.6 / 7.2 | 20 | intergenic (‑72/‑49) | rcnR/rcnA | transcriptional repressor of rcnA/membrane protein conferring nickel and cobalt resistance |
| Reads supporting (aligned to +/- strand): ref base . (1/3); new base G (11/5); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
TGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑GGG‑G‑G‑GGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATG‑ACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCAT > NC_000913/2185735‑2185997 | tGGCCTTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAATACCTGGTTAACAAGAATATAAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTATT‑ggg‑g‑g < 1:64322/139‑1 (MQ=255) tgCCCTGAATGTTTCTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGAAATGGCAACACCTGGTTAACAAGAAAATTAAAAATCATAGCACTATTAATCGGCTGGGGGGTAGTATCAGGTACT‑ggg‑g‑g‑g < 1:117269/138‑1 (MQ=255) cttcagcTTAGTGGCACGTGCTTTCAGTTTCTGTTTATCACGGTTTGAATGAGTCATAGGAATACCTGGTTAACAAAAATAAGAAAGATCATAGCACTATTAATCTTCGGGGGGGTAGTATCAGGTACT‑ggg‑g‑g‑gggag < 2:463327/133‑4 (MQ=255) cACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTTATCTACTGGTGGGTAGTATCAGGTACT‑GGG‑G‑G‑GGAGTAGAATCAGATTGcc < 1:750667/139‑1 (MQ=255) tttaGTTTTTTTTTATAACGGAGTGTATGACACATGGCAACTCCTTGTTAACAAAAATATGAAAAATCATAGCATTATTAATCGACTGGGGGGTAGTATCAAGTAATGGGG‑G‑G‑GGAGTAGAATCAGATTGCCGAATTaa < 1:384742/136‑1 (MQ=255) aTGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAGTCTACTGGGGGGTAGTATCAGGTACT‑ggg‑g‑g‑gggag < 1:332745/84‑4 (MQ=255) aTGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGG‑G‑G‑GGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATG‑ACCGAATTTACaa > 2:528443/1‑139 (MQ=255) aTGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑ggg‑g‑g‑gggag > 2:332745/1‑81 (MQ=255) ggCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGG‑G‑G‑GGAGGTGGAACAAATTTGCGAATTTAAACTAAAAAATATTATTATGAACGGAATTAACact > 2:840364/1‑137 (MQ=255) cTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGG‑G‑G‑GGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATG‑ACCGAATTTACAACTCTTCTTCa > 2:1205597/1‑139 (MQ=255) cAAGAATATGAAAAAGCATAGCACTATTAATCCACTGGGCGGTAGTATCAGGTACT‑GGG‑G‑GCGGAGTAGACTCAGTATGCCGAAGTAATACTATGAATTACTATCATG‑ACCGAATTTACAACTCTACTGCAGCAAGGaa > 1:184797/1‑139 (MQ=255) attagagacaggcTACTGGGTGTTAGTATAAGTTCCTGGGG‑G‑G‑GGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATG‑ACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATccc < 1:269173/127‑1 (MQ=255) tataAAACTACTGGGGGGTAGAAAGAAGTACT‑GGGAG‑G‑AGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATG‑ACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAgcg < 1:528443/139‑1 (MQ=255) cTACTGGGGGGTAGTATCAGGTACTGGGG‑G‑G‑GGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATG‑ACCGAATTTACAACTCTTCTTCAGCAAGGAAACGc > 1:945638/1‑112 (MQ=255) cTACTGGGGGGTAGTATCAGGTACTGGGG‑G‑G‑GGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATG‑ACCGAATTTACAACTCTTCTTCAGCAAGGAAACGc < 2:945638/112‑1 (MQ=255) cTGGGGGGTAGTATCAGGTACTGGGG‑G‑G‑GGCGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATG‑ACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACt > 2:516379/1‑139 (MQ=255) cTGGGGGGTAGTATCAGGTACTGGGG‑G‑G‑GGAGTAGAATCAGATTGCCGAATTAATACTaa > 2:1121085/1‑60 (MQ=255) cTGGGGGGTAGTATCAGGTACTGGGG‑G‑G‑GGAGTAGAATCAGATTGCCGAATTAATACTaa < 1:1121085/60‑1 (MQ=255) cTGGGGGGTAGTATCAGGTACTGGGG‑G‑G‑GGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATG‑ACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACt > 1:750499/1‑139 (MQ=255) gtagtaTCAGGTACTGGGG‑G‑G‑GGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATG‑ACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCtctgtctcttat > 2:269173/1‑128 (MQ=255) tagtaTCAGGTACTGGGG‑G‑G‑GGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATG‑ACCGAATTTACAActc > 2:415431/1‑82 (MQ=255) tagtaTCAGGTACTGGGG‑G‑G‑GGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATG‑ACCGAATTTACAActc < 1:415431/82‑1 (MQ=255) gtaTCAGGTACTGGGG‑G‑G‑GGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATG‑ACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCtt > 2:839577/1‑139 (MQ=255) tCAGGTACTGGGG‑G‑G‑GGAGGAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATG‑ACCGGATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTAATTGGTGCGCTTCAt > 1:1117006/1‑139 (MQ=255) tCAGATACT‑GGG‑GAG‑GAAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATG‑ACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCAt < 2:750499/139‑1 (MQ=255) | TGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑GGG‑G‑G‑GGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATG‑ACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCAT > NC_000913/2185735‑2185997 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |