Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,447,891	G→A	*1260* (TAG→TAA)	tamB →	translocation and assembly module for autotransporter export, inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,447,891	0	G	A	100.0%	264.3 / NA	81	*1260* (TAG→TAA)	tamB	translocation and assembly module for autotransporter export, inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/41);  total (40/41)

TGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGG  >  NC_000913/4447770‑4448021                                                                                                                          |                                                                                                                                   tGGGTATATTTGACTCTATAGCAAGACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCt                                                                                                                  >  1:825692/1‑139 (MQ=255)  gggTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTa                                                                                                                 >  2:179883/1‑139 (MQ=255)           tGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGttt                                                                                                        <  1:179883/139‑1 (MQ=255)           tGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGttt                                                                                                        <  2:825692/139‑1 (MQ=255)            gACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTa                                                                                                       <  2:1119566/139‑1 (MQ=255)             aCTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTAc                                                                                                      >  2:279304/1‑139 (MQ=255)              ctctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACg                                                                                                     >  2:434732/1‑139 (MQ=255)                ctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGcc                                                                                                   <  2:321280/139‑1 (MQ=255)                ctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGcc                                                                                                   <  2:713415/139‑1 (MQ=255)                           cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGTAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                        >  2:1054003/1‑139 (MQ=255)                           cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTc                                                                                                                   <  1:562257/112‑1 (MQ=255)                           cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTc                                                                                                                   >  2:562257/1‑112 (MQ=255)                           cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTa                                                                                                       >  1:944100/1‑124 (MQ=255)                           cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTa                                                                                                       <  2:944100/124‑1 (MQ=255)                           cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                        >  2:945839/1‑139 (MQ=255)                           cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                        >  2:287526/1‑139 (MQ=255)                           cTCAAGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGCA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                        >  1:709305/1‑139 (MQ=255)                             cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                      >  1:593947/1‑139 (MQ=255)                                gttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAg                                                                                   >  1:881182/1‑139 (MQ=255)                                   acgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                      <  1:13127/133‑1 (MQ=255)                                   acgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                      >  2:13127/1‑133 (MQ=255)                                    cgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCAc                                                                               <  2:709305/139‑1 (MQ=255)                                          cGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATg                                                                         >  1:1161427/1‑139 (MQ=255)                                          cGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATg                                                                         >  2:466487/1‑139 (MQ=255)                                              tGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCa                                                                     >  1:684471/1‑139 (MQ=255)                                                   ccTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                                                <  2:863598/139‑1 (MQ=255)                                                       aGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGt                                                            >  2:449201/1‑139 (MQ=255)                                                          tataTCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTAc                                                         <  2:1161427/139‑1 (MQ=255)                                                            tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTg                                                       <  1:287526/139‑1 (MQ=255)                                                             atCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTc                                                                                 >  2:265761/1‑112 (MQ=255)                                                             atCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTc                                                                                 <  1:265761/112‑1 (MQ=255)                                                               cTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                                                    <  2:593947/139‑1 (MQ=255)                                                                 ggAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGa                                                  >  1:524461/1‑139 (MQ=255)                                                                  gAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAt                                                 >  2:302723/1‑139 (MQ=255)                                                                      ccGTTTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGAGATTTCa                                             >  1:93102/1‑139 (MQ=255)                                                                      ccGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCa                                             <  1:845014/139‑1 (MQ=255)                                                                        gtgtCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                      >  2:1079012/1‑96 (MQ=255)                                                                        gtgtCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                      <  1:1079012/96‑1 (MQ=255)                                                                         tgtCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTa                                          <  1:1054003/139‑1 (MQ=255)                                                                            cTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                      <  2:431723/92‑1 (MQ=255)                                                                            cTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                      >  1:431723/1‑92 (MQ=255)                                                                             tGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGa                                      <  1:449201/139‑1 (MQ=255)                                                                                    gACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                               <  1:1073374/139‑1 (MQ=255)                                                                                    gACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                               <  2:684471/139‑1 (MQ=255)                                                                                     aCCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACgg                                                                                                              <  2:912194/59‑1 (MQ=255)                                                                                     aCCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACgg                                                                                                              >  1:912194/1‑59 (MQ=255)                                                                                     aCCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAcc                              <  1:971595/139‑1 (MQ=255)                                                                                       cAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg                            <  1:302723/139‑1 (MQ=255)                                                                                         ggCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCacaaa                                                                                              <  2:279673/71‑1 (MQ=255)                                                                                         ggCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCacaaa                                                                                              >  1:279673/1‑71 (MQ=255)                                                                                         ggCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTAt                                         >  2:823196/1‑124 (MQ=255)                                                                                         ggCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTAt                                         <  1:823196/124‑1 (MQ=255)                                                                                         ggCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGtt                          >  1:706194/1‑139 (MQ=255)                                                                                          gCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACgg                                                                                                              >  2:266443/1‑54 (MQ=255)                                                                                          gCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACgg                                                                                                              <  1:266443/54‑1 (MQ=255)                                                                                          gCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                                                >  2:113468/1‑100 (MQ=255)                                                                                          gCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                                                <  1:113468/100‑1 (MQ=255)                                                                                          gCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg                         >  2:1030353/1‑139 (MQ=255)                                                                                          gCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg                         >  2:317582/1‑139 (MQ=255)                                                                                            aCTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGta                       <  1:434732/139‑1 (MQ=255)                                                                                            aCTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGta                       <  1:945839/139‑1 (MQ=255)                                                                                                ggattGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTc                                                                                 >  2:922280/4‑78 (MQ=255)                                                                                                ggattGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTc                                                                                 <  1:922280/75‑1 (MQ=255)                                                                                                  attGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCa                                             <  1:314898/111‑1 (MQ=255)                                                                                                  attGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCa                                             >  2:314898/2‑112 (MQ=255)                                                                                                  attGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGcc                  <  2:706194/138‑1 (MQ=255)                                                                                                     gCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa                             <  1:280558/125‑1 (MQ=255)                                                                                                     gCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa                             >  2:280558/1‑125 (MQ=255)                                                                                                       tctATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGc             <  2:524461/139‑1 (MQ=255)                                                                                                           tCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTgg                                                                            >  2:784681/1‑72 (MQ=255)                                                                                                           tCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                               >  2:221933/1‑117 (MQ=255)                                                                                                           tCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                               <  1:221933/117‑1 (MQ=255)                                                                                                           tCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCAc          >  2:923874/1‑138 (MQ=255)                                                                                                           tCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCAc          <  1:923874/138‑1 (MQ=255)                                                                                                           tCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCAATgg                                                                            <  1:784681/72‑1 (MQ=255)                                                                                                                tCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCa    >  1:14634/1‑139 (MQ=255)                                                                                                                 cGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                      <  1:1107021/56‑1 (MQ=255)                                                                                                                 cGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                      >  2:1107021/1‑56 (MQ=255)                                                                                                                 cGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATa                                    <  2:652777/106‑1 (MQ=255)                                                                                                                 cGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATa                                    >  1:652777/1‑106 (MQ=255)                                                                                                                  gAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAgg  <  1:317582/139‑1 (MQ=255)                                                                                                                          |                                                                                                                                   TGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGG  >  NC_000913/4447770‑4448021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑