Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,006,415	Δ1 bp	coding (464/543 nt)	zapC →	FtsZ stabilizer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,006,413	0	G	.	100.0%	201.1 / NA	50	coding (462/543 nt)	zapC	FtsZ stabilizer
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (25/25);  total (25/25)

GGGCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTG  >  NC_000913/1006278‑1006545                                                                                                                                        |                                                                                                                                     gggCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTggcg                                                                                                                                   <  2:1092831/139‑3 (MQ=255)   gCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTggcgac                                                                                                                                 <  1:483607/139‑5 (MQ=255)                                aCTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAAc                                                                                                              <  2:644289/128‑1 (MQ=255)                                aCTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAAc                                                                                                              >  1:644289/1‑128 (MQ=255)                                   cACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAAcc                                                                                                <  2:1524999/139‑1 (MQ=255)                                         cAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGa                                                                                                            <  1:104648/121‑1 (MQ=255)                                         cAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGa                                                                                                            >  2:104648/1‑121 (MQ=255)                                         cAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                                                                               >  2:562437/1‑134 (MQ=255)                                         cAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                                                                               <  1:562437/134‑1 (MQ=255)                                         cAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGt                                                                                          >  1:627191/1‑139 (MQ=255)                                            gTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTaa                                                                                       >  1:795916/1‑139 (MQ=255)                                                      tGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTggcga                                                                                                                                  <  1:1565723/87‑4 (MQ=255)                                                      tGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTggcga                                                                                                                                  >  2:1565723/1‑84 (MQ=255)                                                      tGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGc                                                                             >  2:381951/1‑139 (MQ=255)                                                       ggTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTg                                                                                  >  1:1019055/1‑133 (MQ=255)                                                       ggTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTg                                                                                  <  2:1019055/133‑1 (MQ=255)                                                                  cTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTggcga                                                                                                                                  >  2:1420871/1‑72 (MQ=255)                                                                  cTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTggcga                                                                                                                                  <  1:1420871/75‑4 (MQ=255)                                                                    ggCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTggcgac                                                                                                                                 <  2:801853/74‑5 (MQ=255)                                                                    ggCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTggcgac                                                                                                                                 >  1:801853/1‑70 (MQ=255)                                                                     gCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGa                                                                                                            >  2:1730862/1‑93 (MQ=255)                                                                     gCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGa                                                                                                            <  1:1730862/93‑1 (MQ=255)                                                                     gCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCt                                                                     <  1:941069/132‑1 (MQ=255)                                                                     gCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCt                                                                     >  2:941069/1‑132 (MQ=255)                                                                         aaaTGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAgg                                                                                           <  2:1610865/106‑1 (MQ=255)                                                                         aaaTGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAgg                                                                                           >  1:1610865/1‑106 (MQ=255)                                                                                   cTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACgcgc                                                <  1:1514055/139‑1 (MQ=255)                                                                                    tATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGcc                                               >  2:780825/1‑139 (MQ=255)                                                                                            ctgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACGGGCAGTTTAACGCGCCAACTTAAg                                       >  2:1482144/1‑139 (MQ=255)                                                                                              ggCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTg                                     >  2:170814/1‑139 (MQ=255)                                                                                                cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTaaaa                                                                                                                      >  1:1213089/1‑56 (MQ=255)                                                                                                cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTaaaa                                                                                                                      <  2:1213089/56‑1 (MQ=255)                                                                                                cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCa                                   <  2:627191/139‑1 (MQ=255)                                                                                                cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCa                                   <  1:780825/139‑1 (MQ=255)                                                                                                  caACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGt                                 <  2:795916/139‑1 (MQ=255)                                                                                                      ccTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTc                             <  2:733688/139‑1 (MQ=255)                                                                                                          cGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTa                         >  1:1813183/1‑139 (MQ=255)                                                                                                               ttATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTa                                                                                                                         >  2:1454830/1‑38 (MQ=255)                                                                                                               ttATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTa                                                                                                                         <  1:1454830/38‑1 (MQ=255)                                                                                                               ttATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGa                                                                  <  2:866029/93‑1 (MQ=255)                                                                                                               ttATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGa                                                                  >  1:866029/1‑93 (MQ=255)                                                                                                                  ttGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTa                 <  1:381951/139‑1 (MQ=255)                                                                                                                  ttGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTa                 <  1:1282389/139‑1 (MQ=255)                                                                                                                    gCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTaaaa                                                                                                                      >  1:1142296/1‑36 (MQ=255)                                                                                                                    gCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTaaaa                                                                                                                      <  2:1142296/36‑1 (MQ=255)                                                                                                                       ggTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGa                                                                                                            <  1:1193734/43‑1 (MQ=255)                                                                                                                       ggTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCAGGAACGa                                                                                                            >  2:1193734/1‑43 (MQ=255)                                                                                                                         tCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCAt                                                                                                                  >  2:561362/1‑35 (MQ=255)                                                                                                                         tCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCAt                                                                                                                  <  1:561362/35‑1 (MQ=255)                                                                                                                         tCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGa                                                                                                            <  2:1507401/41‑1 (MQ=255)                                                                                                                         tCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGa                                                                                                            >  1:1507401/1‑41 (MQ=255)                                                                                                                            cgcgATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                                                                               <  2:267426/51‑1 (MQ=255)                                                                                                                            cgcgATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                                                                               >  1:267426/1‑51 (MQ=255)                                                                                                                            cgcgATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCa            >  2:1655657/1‑134 (MQ=255)                                                                                                                            cgcgATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCa            <  1:1655657/134‑1 (MQ=255)                                                                                                                            cgcgATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGa        <  2:15450/138‑1 (MQ=255)                                                                                                                            cgcgATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGa        >  1:15450/1‑138 (MQ=255)                                                                                                                                 tGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTg  >  2:604194/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                     GGGCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTG  >  NC_000913/1006278‑1006545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑