Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	321,564	(G)7→6	intergenic (+483/‑44)	rclR → / → ykgE	reactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein, putative electron transport chain YkgEFG component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	321,558	0	G	.	100.0%	266.2 / NA	66	intergenic (+477/‑50)	rclR/ykgE	reactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein, putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (36/30);  total (36/30)

ATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAAC  >  NC_000913/321434‑321692                                                                                                                             |                                                                                                                                       aTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCgg                                                                                                                         >  2:1871824/1‑139 (MQ=255)  tAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCggg                                                                                                                        <  2:1846335/139‑1 (MQ=255)                gCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATg                                                                                                          <  1:1243769/139‑1 (MQ=255)                gCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATg                                                                                                          >  1:1405373/1‑139 (MQ=255)                 cATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTggggg                                                                                                                                    >  1:377752/1‑113 (MQ=255)                 cATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTggggg                                                                                                                                    <  2:377752/113‑1 (MQ=255)                 cATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACAt                                                                                                                            >  1:1836678/1‑120 (MQ=255)                 cATTGGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACAt                                                                                                                            <  2:1836678/120‑1 (MQ=255)                                               tGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTa                                                                                                     <  2:1424938/113‑1 (MQ=255)                                               tGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTa                                                                                                     >  1:1424938/1‑113 (MQ=255)                                                          tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:30630/139‑1 (MQ=255)                                                          tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  2:698758/139‑1 (MQ=255)                                                           gAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAcc                                                               >  2:1017004/1‑139 (MQ=255)                                                                gtggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTAt                                                          <  2:1405373/139‑1 (MQ=255)                                                                 tggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATggg                                                                                                              >  1:211354/1‑86 (MQ=255)                                                                 tggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATggg                                                                                                              <  2:211354/86‑1 (MQ=255)                                                                   gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa                                                                                                            <  2:1353101/86‑1 (MQ=255)                                                                   gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa                                                                                                            >  1:1353101/1‑86 (MQ=255)                                                                   gtATCTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCACTTTCTTTGTCACCTGTATTgg                                                       >  1:1094107/1‑139 (MQ=255)                                                                           cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTACTGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  2:1751100/1‑139 (MQ=255)                                                                           cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  2:755309/122‑1 (MQ=255)                                                                           cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                >  1:755309/1‑122 (MQ=255)                                                                              gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa                                                                                                            <  2:330685/75‑1 (MQ=255)                                                                              gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa                                                                                                            >  1:330685/1‑75 (MQ=255)                                                                              gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                >  2:968313/1‑119 (MQ=255)                                                                              gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:968313/119‑1 (MQ=255)                                                                                   ttAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCa                                       >  2:150969/1‑139 (MQ=255)                                                                                       aaaaGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGaa                                   <  1:1871824/139‑1 (MQ=255)                                                                                            aaCGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCa                              <  1:1751100/139‑1 (MQ=255)                                                                                            aaCGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCa                              >  1:1846185/1‑139 (MQ=255)                                                                                             aCGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACa                                                                                                                             >  2:1607746/1‑43 (MQ=255)                                                                                             aCGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACa                                                                                                                             <  1:1607746/43‑1 (MQ=255)                                                                                             aCGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATg                                                                                                          >  2:1594466/1‑62 (MQ=255)                                                                                             aCGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATg                                                                                                          <  1:1594466/62‑1 (MQ=255)                                                                                             aCGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCAc                             >  2:213367/1‑139 (MQ=255)                                                                                              cGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTa                                                                                                     >  1:1266490/1‑66 (MQ=255)                                                                                              cGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTa                                                                                                     <  2:1266490/66‑1 (MQ=255)                                                                                              cGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACg                            >  2:1750369/1‑139 (MQ=255)                                                                                                ccATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACgag                          >  2:271201/1‑139 (MQ=255)                                                                                                ccATATTTATTGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACgag                          <  2:908443/139‑1 (MQ=255)                                                                                                   tatttattGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAg                                                                                                       >  2:626851/1‑59 (MQ=255)                                                                                                   tatttattGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAg                                                                                                       <  1:626851/59‑1 (MQ=255)                                                                                                   tatttattGATGATTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACt                       <  1:213367/139‑1 (MQ=255)                                                                                                           gatgatTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGaa                                   >  2:87446/1‑119 (MQ=255)                                                                                                           gatgatTGATCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGaa                                   <  1:87446/119‑1 (MQ=255)                                                                                                             tgattgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGaa                                   >  2:1197150/1‑117 (MQ=255)                                                                                                             tgattgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGaa                                   <  1:1197150/117‑1 (MQ=255)                                                                                                             tgattgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACg                            <  2:1857048/124‑1 (MQ=255)                                                                                                             tgattgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACg                            >  1:1857048/1‑124 (MQ=255)                                                                                                             tgattgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTgctgct             >  2:561006/1‑139 (MQ=255)                                                                                                              gattgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTa            <  1:150969/139‑1 (MQ=255)                                                                                                              gattgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTa            >  1:938803/1‑139 (MQ=255)                                                                                                               attgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATgg                                                                                                 >  1:190570/1‑53 (MQ=255)                                                                                                               attgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATgg                                                                                                 <  2:190570/53‑1 (MQ=255)                                                                                                               attgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGt                                                                   <  1:110327/83‑1 (MQ=255)                                                                                                               attgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGt                                                                   >  2:110327/1‑83 (MQ=255)                                                                                                               attgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGt                                                      >  2:622367/1‑96 (MQ=255)                                                                                                               attgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGt                                                      <  1:622367/96‑1 (MQ=255)                                                                                                               attgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTAc           >  2:1012466/1‑139 (MQ=255)                                                                                                               attgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTAc           >  1:69756/1‑139 (MQ=255)                                                                                                               attgatCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTAc           >  1:1830861/1‑139 (MQ=255)                                                                                                                   atCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGtgctg               >  2:522565/1‑131 (MQ=255)                                                                                                                   atCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGtgctg               <  1:522565/131‑1 (MQ=255)                                                                                                                      ccccGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCttt                                                                     <  2:1317841/74‑1 (MQ=255)                                                                                                                      ccccGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCttt                                                                     >  1:1317841/1‑74 (MQ=255)                                                                                                                        ccGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAg                                     <  2:1353145/104‑1 (MQ=255)                                                                                                                        ccGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAg                                     >  1:1353145/1‑104 (MQ=255)                                                                                                                        ccGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAAc  >  1:1472040/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                       ATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAAC  >  NC_000913/321434‑321692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑