Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	401,548	(G)5→4	coding (163/261 nt)	iraP →	anti‑RssB factor, RpoS stabilzer during Pi starvation, anti‑adapter protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	401,544	0	G	.	100.0%	295.9 / NA	73	coding (159/261 nt)	iraP	anti‑RssB factor, RpoS stabilzer during Pi starvation, anti‑adapter protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (37/36);  total (37/36)

GTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGT  >  NC_000913/401406‑401678                                                                                                                                           |                                                                                                                                       gttgttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTagag                                                                                                                                        <  2:440564/139‑1 (MQ=255)      tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                   <  2:1186116/139‑3 (MQ=255)      tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                   <  2:1464626/139‑3 (MQ=255)          tAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGt                                                                                                                              >  2:413424/1‑139 (MQ=255)            aGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATACTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTAc                                                                                                                            <  1:1231016/139‑1 (MQ=255)              cTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGa                                                                                                                          >  1:1731571/1‑139 (MQ=255)                tGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                   >  1:34707/1‑127 (MQ=255)                tGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                   <  2:34707/129‑3 (MQ=255)                  cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTa                                                                                                                      >  1:286364/1‑139 (MQ=255)                  cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTa                                                                                                                      >  2:1861472/1‑139 (MQ=255)                        aagaagaagAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGccc                                                                                                                <  2:1816817/139‑1 (MQ=255)                                   tCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTAc                                                                                                                            <  1:489524/116‑1 (MQ=255)                                   tCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTAc                                                                                                                            >  2:489524/1‑116 (MQ=255)                                   tCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCTCGATGCCAGCAt                                                                                                     >  1:1120342/1‑139 (MQ=255)                                   tCGAAAGAACTGTGTGCGCAGGTAGAAGC‑‑TGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCa                                                                                                      <  2:971766/136‑1 (MQ=255)                                   tCGAAAGAACTGTGTGCGCAGGTAGAAGC‑‑TGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCa                                                                                                      >  1:971766/1‑136 (MQ=255)                                            cTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacga                                                                                            <  1:1626345/139‑1 (MQ=255)                                               tgtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGAcgacga                                                                                         >  2:1023364/1‑139 (MQ=255)                                                 tgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATa                                                                                       <  2:649856/139‑1 (MQ=255)                                                 tgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCACTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATa                                                                                       >  1:1858965/1‑139 (MQ=255)                                                       ggTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgc                                                                                 >  2:1796706/1‑139 (MQ=255)                                                         tAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgctg                                                                               >  2:1329969/1‑139 (MQ=255)                                                         tAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgctg                                                                               >  2:700549/1‑139 (MQ=255)                                                            aaGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGctgctg                                                                            <  1:1329969/139‑1 (MQ=255)                                                              gCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTgcg                                                                          <  1:1796706/139‑1 (MQ=255)                                                                tttGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGcgcg                                                                        <  1:700549/139‑1 (MQ=255)                                                                tttGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGcgcg                                                                        <  1:1591226/139‑1 (MQ=255)                                                                 ttGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGa                                                                       <  1:497061/139‑1 (MQ=255)                                                                 ttGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCACTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGa                                                                       <  2:1858965/139‑1 (MQ=255)                                                                  tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacg                                                                                             >  1:1637324/1‑116 (MQ=255)                                                                  tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacg                                                                                             <  2:1637324/116‑1 (MQ=255)                                                                  tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  2:1512023/1‑139 (MQ=255)                                                                  tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  1:585615/1‑139 (MQ=255)                                                                            cGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaaga                                                            <  2:286364/139‑1 (MQ=255)                                                                              tCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTagag                                                                                                                                        <  2:916380/62‑1 (MQ=255)                                                                              tCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTagag                                                                                                                                        >  1:916380/1‑62 (MQ=255)                                                                              tCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaagaag                                                          <  2:1777237/139‑1 (MQ=255)                                                                                  tGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTc                                                                                                   >  1:1809665/1‑94 (MQ=255)                                                                                  tGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTc                                                                                                   <  2:1809665/94‑1 (MQ=255)                                                                                  tGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTAt                                                      <  1:413424/139‑1 (MQ=255)                                                                                  tGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTCGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTAt                                                      >  1:1207479/1‑139 (MQ=255)                                                                                        gCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGt                                                                 <  2:1082851/122‑1 (MQ=255)                                                                                        gCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGt                                                                 >  1:1082851/1‑122 (MQ=255)                                                                                          ttCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAg                                                                                  >  2:1494136/1‑103 (MQ=255)                                                                                          ttCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAg                                                                                  <  1:1494136/103‑1 (MQ=255)                                                                                            cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAg                                                                                                        <  1:308614/79‑1 (MQ=255)                                                                                            cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAg                                                                                                        >  2:308614/1‑79 (MQ=255)                                                                                            cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTgcg                                                                          <  2:1056389/109‑1 (MQ=255)                                                                                            cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTgcg                                                                          >  1:1056389/1‑109 (MQ=255)                                                                                            cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATcc                                            >  2:1268251/1‑139 (MQ=255)                                                                                            cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATc                                             <  2:778425/138‑1 (MQ=255)                                                                                            cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATc                                             >  1:778425/1‑138 (MQ=255)                                                                                              cAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacga                                                                                            >  1:1655484/1‑89 (MQ=255)                                                                                              cAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacga                                                                                            <  2:1655484/89‑1 (MQ=255)                                                                                                atatGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGt                                        <  1:1023364/139‑1 (MQ=255)                                                                                                 tatGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGcc                                                                                                          >  2:1105673/1‑72 (MQ=255)                                                                                                 tatGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGCACGAGGTAAAGCCCGATGcc                                                                                                          <  1:1105673/72‑1 (MQ=255)                                                                                                   tGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                   <  2:460537/46‑3 (MQ=255)                                                                                                   tGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                   >  1:460537/1‑44 (MQ=255)                                                                                                      cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                   <  2:246113/43‑3 (MQ=255)                                                                                                      cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                   >  1:246113/1‑41 (MQ=255)                                                                                                      cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTga                                                                                               <  2:592409/78‑1 (MQ=255)                                                                                                      cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTga                                                                                               >  1:592409/1‑78 (MQ=255)                                                                                                      cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTaa                                  >  1:897808/1‑139 (MQ=255)                                                                                                      cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTaa                                  >  2:1427569/1‑139 (MQ=255)                                                                                                           aaTGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCa                                                                                                      >  1:1095491/1‑66 (MQ=255)                                                                                                           aaTGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCa                                                                                                      <  2:1095491/66‑1 (MQ=255)                                                                                                             tGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTa                           >  1:263677/1‑139 (MQ=255)                                                                                                             tGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTa                           <  1:1268251/139‑1 (MQ=255)                                                                                                                ccAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATc                        <  2:1731571/139‑1 (MQ=255)                                                                                                                       cGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATc                                             <  2:148598/111‑1 (MQ=255)                                                                                                                       cGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATc                                             >  1:148598/1‑111 (MQ=255)                                                                                                                       cGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCaa                 >  2:1366798/1‑139 (MQ=255)                                                                                                                         gTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGt                                        <  2:1573075/114‑1 (MQ=255)                                                                                                                         gTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGt                                        >  1:1573075/1‑114 (MQ=255)                                                                                                                         gTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACa               >  1:1289668/1‑139 (MQ=255)                                                                                                                          ttgattgaTCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  2:263677/139‑1 (MQ=255)                                                                                                                          ttgattgaTCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  1:1366798/139‑1 (MQ=255)                                                                                                                           tgattgatCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGc             <  1:1512023/139‑1 (MQ=255)                                                                                                                           tgattgatCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGc             <  2:1289668/139‑1 (MQ=255)                                                                                                                                 atCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCAt       >  2:512711/1‑139 (MQ=255)                                                                                                                                   cAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTga                                                                                               <  1:923895/49‑1 (MQ=255)                                                                                                                                   cAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTga                                                                                               >  2:923895/1‑49 (MQ=255)                                                                                                                                      gTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGt  >  2:332899/1‑139 (MQ=255)                                                                                                                                           |                                                                                                                                       GTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGT  >  NC_000913/401406‑401678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑