| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,920,205 | (T)7→8 | intergenic (+62/‑18) | yebT → / → rsmF | MCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,920,198 | 1 | . | T | 98.1% | 164.6 / ‑3.8 | 52 | intergenic (+55/‑25) | yebT/rsmF | MCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (27/24); total (27/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
GGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑TTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCC > NC_000913/1920068‑1920328 | ggAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tttt‑tttt < 1:1476366/139‑2 (MQ=255)ggAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tttt‑tttt > 1:388773/1‑138 (MQ=255) aaGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tttt‑ttttgc < 2:388773/139‑4 (MQ=255) gCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGAGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAg < 1:1329451/139‑1 (MQ=255) acacTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tttt‑ttttgcc < 2:691996/133‑5 (MQ=255) acacTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tttt‑ttttgcc > 1:691996/1‑129 (MQ=255) cTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACa < 2:365695/139‑1 (MQ=255) ccTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGc > 1:593002/1‑139 (MQ=255) cAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGc < 1:829/139‑1 (MQ=255) aGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAaca < 1:1518526/139‑1 (MQ=255) aaCCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tttt‑ttttgcca > 2:597033/1‑106 (MQ=255) aaCCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tttt‑ttttgcca < 1:597033/111‑6 (MQ=255) gagTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTAttt > 2:1199493/1‑139 (MQ=255) gTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGAc < 1:517826/139‑1 (MQ=255) tGAATGGGGAACTGAGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTCTATGCTAAACTGCGCCCCTG‑TTTTCTTTGCCAGTGGTACATGCTCGTGGCCGAACACACCGTTTATTTCCCGGACg < 2:1149262/139‑1 (MQ=255) gAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tttt‑ttttgc < 1:1734066/85‑4 (MQ=255) gAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tttt‑ttttgc > 2:1734066/1‑82 (MQ=255) cTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTc > 1:353188/1‑134 (MQ=255) cTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTc < 2:353188/134‑1 (MQ=255) tGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGacac > 2:521394/1‑139 (MQ=255) gcgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGAcaca > 2:1457423/1‑139 (MQ=255) cgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACaa > 1:1278764/1‑139 (MQ=255) cccAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATgcg < 1:1199493/139‑1 (MQ=255) ccAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATgcgc < 1:521394/139‑1 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGccc > 2:26448/1‑86 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGccc < 1:26448/86‑1 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCg > 1:1486284/1‑136 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCg < 2:1486284/136‑1 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATg > 1:549988/1‑139 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATg > 2:377509/1‑139 (MQ=255) cccACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAacacac > 1:1341442/1‑92 (MQ=255) cccACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAacacac < 2:1341442/92‑1 (MQ=255) cTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tttt‑tttt > 2:932068/1‑56 (MQ=255) cTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tttt‑tttt < 1:932068/57‑2 (MQ=255) tGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCg > 1:1206997/1‑139 (MQ=255) cTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGAc > 2:152183/1‑139 (MQ=255) tCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACg < 2:1206997/139‑1 (MQ=255) gCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCAtt < 2:1278764/139‑1 (MQ=255) cccgcgcCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCg > 1:1068601/4‑127 (MQ=255) cccgcgcCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCg < 2:1068601/124‑1 (MQ=255) ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGc > 1:660934/1‑117 (MQ=255) ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGc < 2:660934/117‑1 (MQ=255) ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCAttt > 1:1131196/1‑139 (MQ=255) cTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCgg < 1:196587/88‑1 (MQ=255) cTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCgg > 2:196587/1‑88 (MQ=255) aGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCa < 2:1091103/59‑1 (MQ=255) aGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAaca > 2:663574/1‑62 (MQ=255) aGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAaca < 1:663574/62‑1 (MQ=255) aGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTAt > 1:546828/1‑72 (MQ=255) aGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTAt < 2:546828/72‑1 (MQ=255) aGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCg > 1:402473/1‑78 (MQ=255) aGCGTTTATGCTAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCg < 2:402473/78‑1 (MQ=255) aGCGATTATGCAAAACTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCa > 1:1091103/1‑59 (MQ=255) aaaCTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTtgat < 2:1659978/120‑1 (MQ=255) aaaCTGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTtgat > 1:1659978/1‑120 (MQ=255) tGCGCGCCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAgcg < 1:377509/139‑1 (MQ=255) cgcgcCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAaca > 1:1007889/1‑44 (MQ=38) cgcgcCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAaca < 2:1007889/44‑1 (MQ=255) cgcgcCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGcc > 1:1575716/1‑139 (MQ=255) cgcgcCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGcc > 2:1093551/1‑139 (MQ=255) cgcgcCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGcc > 1:954460/1‑139 (MQ=255) cgcCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTAt > 1:1533601/1‑52 (MQ=255) cgcCTGTTTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTAt < 2:1533601/52‑1 (MQ=255) | GGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑TTTT‑TTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCC > NC_000913/1920068‑1920328 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |