BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,511,746	(A)_8→9	coding (599/906 nt)	yhfZ ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,511,738	1	.	A	98.0%	317.6 / ‑1.6	98	coding (607/906 nt)	yhfZ	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base . (2/0); new base A (53/43); total (55/43)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01

Only 100 of 109 total aligned reads displayed.

GCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGC  >  NC_000913/3511603‑3511871

gcgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaa                                                                                                                                      >  1:1589451/1‑139 (MQ=255)

 cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaa                                                                                                                                     >  1:1715967/1‑139 (MQ=255)

        tctAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaaaaa‑aaca                                                                                                                             <  1:742296/139‑4 (MQ=255)

             tCCCACCATGCTCAGTTCGTTTTCCTCCACCACGTTCCAGATCACCGCATCGACATCCCCTTTACCAATGCGTTTTAAACTCTCGTGATAAGATAGATCTACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGt                                                                                                                        <  1:1177101/139‑1 (MQ=255)

                 aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATg                                                                                                                    >  2:1177101/1‑139 (MQ=255)

                 aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATg                                                                                                                    >  2:1526945/1‑139 (MQ=255)

                 aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATg                                                                                                                    <  2:1437154/139‑1 (MQ=255)

                        tCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCt                                                                                                             >  2:53627/1‑139 (MQ=255)

                            ttCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATc                                                                                                         >  2:190159/1‑139 (MQ=255)

                             tCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGCTcc                                                                                                        >  2:1797878/1‑139 (MQ=255)

                                 tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATccgccg                                                                                                    <  1:662989/139‑1 (MQ=255)

                                    ccGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAAc                                                                                                 <  1:770427/139‑1 (MQ=255)

                                      gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                               <  2:1715967/139‑1 (MQ=255)

                                          ccacGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGCTCCGCCGAACGGTTAt                                                                                           >  2:897994/1‑139 (MQ=255)

                                             cGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCa                                                                                        >  2:817305/1‑139 (MQ=255)

                                                     aTCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                                <  1:1526945/139‑1 (MQ=255)

                                                          cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTTCACATCACTACCGCC‑‑aaaaaaa‑aaca                                                                                                                             >  1:1233610/1‑86 (MQ=255)

                                                          cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt                                                                           >  2:8479/1‑139 (MQ=255)

                                                          cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt                                                                           <  1:1797878/139‑1 (MQ=255)

                                                          cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaaaaa‑aaca                                                                                                                             <  2:1233610/89‑4 (MQ=255)

                                                            cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTc                                                                          >  2:422100/1‑139 (MQ=255)

                                                            cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaaaaa‑aaca                                                                                                                             <  1:1521034/87‑4 (MQ=255)

                                                                   aTCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGCTTTTCTGATccgccg                                                                                                    >  1:251244/1‑105 (MQ=255)

                                                                   aTCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATccgccg                                                                                                    <  2:251244/105‑1 (MQ=255)

                                                                    tCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCCCTACCGCCA‑AAAAAAA‑A‑CATCAGTCATGATTTTCTGATCCGCCGAACGATTATCCAGCCCCACGCGCTTCACGTTTgtg                                                                 >  2:527893/1‑137 (MQ=255)

                                                                    tCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCg                                                                 >  2:1675652/1‑139 (MQ=255)

                                                                     cGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑AAAAAAA‑ACCATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCgg                                                                >  2:836319/1‑139 (MQ=255)

                                                                           tAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGc                                                          >  2:779219/1‑139 (MQ=255)

                                                                           tAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGc                                                          >  1:122201/1‑139 (MQ=255)

                                                                           tAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaaaaa‑aacat                                                                                                                            >  2:1030923/1‑69 (MQ=255)

                                                                           tAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaaaaa‑aacat                                                                                                                            <  1:1030923/73‑5 (MQ=255)

                                                                            aaCAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGAtt                                                                                                                 >  2:1864109/1‑83 (MQ=255)

                                                                            aaCAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGAtt                                                                                                                 <  1:1864109/83‑1 (MQ=255)

                                                                                 tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGttt                                                                    >  2:1450833/1‑123 (MQ=255)

                                                                                 tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGttt                                                                    <  1:1450833/123‑1 (MQ=255)

                                                                                 tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc                                                    >  2:1085696/1‑139 (MQ=255)

                                                                                 tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc                                                    >  2:123787/1‑139 (MQ=255)

                                                                                  gCGTTGTAAACTCTCGTGATAAGAGAGATCGCCTCGTTCCACATCACTACCGCC‑‑AAAAAAACA‑CATCGGTCATGATTTTCTGATCCGCCGaa                                                                                                  >  2:1842583/1‑92 (MQ=255)

                                                                                  gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGaa                                                                                                  <  1:1842583/92‑1 (MQ=255)

                                                                                  gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa                                                                                  <  1:1002585/108‑1 (MQ=255)

                                                                                  gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACg                                                   >  1:558029/1‑139 (MQ=255)

                                                                                  gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACg                                                   >  2:1428731/1‑139 (MQ=255)

                                                                                  gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACg                                                   <  1:123787/139‑1 (MQ=255)

                                                                                  gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGAACCGCCGAACGGTTATCCAGCCCCa                                                                                  >  2:1002585/1‑108 (MQ=255)

                                                                                  gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaaaaa‑aa                                                                                                                               >  2:1562236/1‑62 (MQ=255)

                                                                                  gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaaaaa‑aa                                                                                                                               <  1:1562236/63‑2 (MQ=255)

                                                                                    gTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                                 <  2:1589451/139‑1 (MQ=255)

                                                                                           aCTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCaa                                          >  2:525726/1‑139 (MQ=255)

                                                                                            ctctCGTGATAAGAGAGATCGACTCGTCCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAAc                                         <  1:1085696/139‑1 (MQ=255)

                                                                                             tctcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATccgccg                                                                                                    >  2:683184/1‑79 (MQ=255)

                                                                                               tcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                               <  2:1264570/82‑1 (MQ=255)

                                                                                               tcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                               >  1:1264570/1‑82 (MQ=255)

                                                                                               tcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTgg                                      >  1:1790919/1‑139 (MQ=255)

                                                                                                    aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc                                                            >  2:936509/1‑112 (MQ=255)

                                                                                                    aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc                                                            <  1:936509/112‑1 (MQ=255)

                                                                                                    aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc                                 <  2:1018954/139‑1 (MQ=255)

                                                                                                      aaGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                               <  1:1675652/139‑1 (MQ=255)

                                                                                                      aaGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                               <  1:190159/139‑1 (MQ=255)

                                                                                                      aaGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                               <  1:1420904/139‑1 (MQ=255)

                                                                                                      aaGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                               <  1:566438/139‑1 (MQ=255)

                                                                                                       agagagATCGACTCGTTCCACATCACTACCGCCAAAAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                               <  1:422100/139‑1 (MQ=255)

                                                                                                         agagaTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAAc                                         >  2:1635759/1‑126 (MQ=255)

                                                                                                           agaTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCgg                                                                                                                         >  2:306029/1‑44 (MQ=255)

                                                                                                           agaTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCgg                                                                                                                         <  1:306029/44‑1 (MQ=38)

                                                                                                            gaTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                                    <  1:1321922/128‑1 (MQ=255)

                                                                                                            gaTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                                    >  2:1321922/1‑128 (MQ=255)

                                                                                                             aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCa                                                                                                                      <  1:1792679/45‑1 (MQ=255)

                                                                                                             aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCa                                                                                                                      >  2:1792679/1‑45 (MQ=255)

                                                                                                             aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc                                                            <  1:914598/103‑1 (MQ=255)

                                                                                                             aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc                                                            >  2:914598/1‑103 (MQ=255)

                                                                                                             aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa                        >  1:1182689/1‑139 (MQ=255)

                                                                                                             aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa                        >  1:472213/1‑139 (MQ=255)

                                                                                                             aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCAGTCATGTTTTTCTGATCCGCCGAAc                                                                                                 >  2:1289571/1‑66 (MQ=255)

                                                                                                             aTCGACTCGTTCCACATCACTACCACCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                               <  1:1226258/132‑1 (MQ=255)

                                                                                                              tCGACTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAg                       <  1:525726/139‑1 (MQ=255)

                                                                                                                 aCTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCa                                                                                                                      <  1:585517/41‑1 (MQ=255)

                                                                                                                 aCTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCa                                                                                                                      >  2:585517/1‑41 (MQ=255)

                                                                                                                  cTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt                                                             <  1:885555/97‑1 (MQ=255)

                                                                                                                  cTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt                                                             >  2:885555/1‑97 (MQ=255)

                                                                                                                  cTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc                                                            <  2:164212/98‑1 (MQ=255)

                                                                                                                  cTCGTTCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc                                                            >  1:164212/1‑98 (MQ=255)

                                                                                                                     ggtCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCg                >  1:1127464/3‑139 (MQ=255)

                                                                                                                       tCCACATCACTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGc              <  1:1428731/139‑1 (MQ=255)

                                                                                                                             tCACTACCGCCAAAAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCaa         <  1:1056981/139‑1 (MQ=255)

                                                                                                                                cTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc                                                    <  2:57886/92‑1 (MQ=255)

                                                                                                                                cTACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc                                                    >  1:57886/1‑92 (MQ=255)

                                                                                                                                 tACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGAt                                                                                                          <  1:1496089/37‑1 (MQ=255)

                                                                                                                                 tACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGAt                                                                                                          >  2:1496089/1‑37 (MQ=255)

                                                                                                                                 tACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt                                                             <  2:518949/82‑1 (MQ=255)

                                                                                                                                 tACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt                                                             >  1:518949/1‑82 (MQ=255)

                                                                                                                                 tACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                                 <  2:27985/94‑1 (MQ=255)

                                                                                                                                 tACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                                 >  1:27985/1‑94 (MQ=255)

                                                                                                                                 tACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCg                <  1:1567556/127‑1 (MQ=255)

                                                                                                                                 tACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCg                >  2:1567556/1‑127 (MQ=255)

                                                                                                                                 tACCGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa    >  1:504127/1‑139 (MQ=255)

                                                                                                                                   ccGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa                                                                                  >  1:1383672/1‑59 (MQ=255)

                                                                                                                                   ccGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa                                                                                  <  2:1383672/59‑1 (MQ=255)

                                                                                                                                   ccGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAgc  >  2:402261/1‑139 (MQ=255)

                                                                                                                                   ccGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAgc  >  2:1457610/1‑139 (MQ=255)

                                                                                                                                   ccGCCA‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAgc  >  1:933849/1‑139 (MQ=255)

GCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑AAAAAAA‑A‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGC  >  NC_000913/3511603‑3511871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`