| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,606,330 | (G)8→9 | noncoding (72/87 nt) | leuQ ← | tRNA‑Leu |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,606,322 | 1 | . | G | 86.2% | 168.0 / 19.1 | 58 | noncoding (80/87 nt) | leuQ | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (5/3); new base G (25/25); total (30/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGT > NC_000913/4606187‑4606453 | atatCGCCTCGTTTGGTGCGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑g‑g‑g < 2:1871607/139‑1 (MQ=255) gTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑g‑g‑gggggggactt > 2:185863/1‑134 (MQ=255) tGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGaa < 1:185863/139‑1 (MQ=255) gTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAAcc > 1:1379709/1‑139 (MQ=255) gTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑g‑g‑ggggggggacttg > 2:1529653/1‑129 (MQ=255) gTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGAAATATCAACGTGGTTTTTGGTGCG‑A‑‑g‑g‑ggggggggacttg < 1:1529653/136‑8 (MQ=255) tGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGCG‑‑G‑G‑GGGGGGACTGGAAccc > 2:916210/1‑139 (MQ=18) aGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑g > 1:1128842/1‑117 (MQ=255) aGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑g < 2:1128842/117‑1 (MQ=255) ggggggggACTTGAACCCCCACGTCCGTAAGGACACTATCACCTGAAGCTGGCGCCTCTACCAATTCCGCCACCTTCGCCCACTGTGAGCAATATCAACGGGTTTTTTTGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACg < 1:967437/139‑1 (MQ=18) ggggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACg < 2:311405/139‑1 (MQ=255) gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGt > 1:1170790/1‑139 (MQ=255) gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGt > 2:969750/1‑139 (MQ=255) ttGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGa < 1:969750/139‑1 (MQ=255) aCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACt < 2:1379709/139‑1 (MQ=255) ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑g > 2:1844201/1‑98 (MQ=255) ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑g < 1:1844201/98‑1 (MQ=255) tCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAAcccc > 2:603695/1‑112 (MQ=255) tCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAAcccc < 1:603695/112‑1 (MQ=255) acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg > 2:488569/1‑134 (MQ=255) acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg < 1:488569/134‑1 (MQ=255) acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGt > 2:1749012/1‑139 (MQ=255) aaCACCTGAAGCTAGCGCGTCTACCAATTGCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑G‑GCGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa > 1:1083580/1‑139 (MQ=255) cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAt > 2:1429154/1‑139 (MQ=255) gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGaca > 2:1407437/1‑107 (MQ=255) gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGaca < 1:1407437/107‑1 (MQ=255) tAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc > 2:1769967/1‑137 (MQ=255) tAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACGTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 1:1769967/137‑1 (MQ=255) cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt > 1:1580775/1‑139 (MQ=255) gcgTCTACCAATCCCGCCCCCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt < 2:1750559/139‑1 (MQ=255) tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc > 1:1504134/1‑139 (MQ=255) cTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTTAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa < 2:1170790/139‑1 (MQ=255) tCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACt > 1:1556827/1‑88 (MQ=255) tCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACt < 2:1556827/88‑1 (MQ=255) ccGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGCACACTAACACCTGAAGCTAGCGCGTCTACCAAt < 2:1838573/115‑1 (MQ=255) ccGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGCACACTAACACCTGAAGCTAGCGCGTCTACCAAt > 1:1838573/1‑115 (MQ=255) cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa < 2:1813918/88‑1 (MQ=255) cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa > 1:1813918/1‑88 (MQ=255) ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTc > 2:1095856/1‑139 (MQ=255) ttCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa < 2:985225/105‑1 (MQ=255) ttCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa > 1:985225/1‑105 (MQ=255) tGCGTGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑g‑g‑g > 2:1384092/1‑35 (MQ=255) tGCGAGCAATATCAACGTGGTTTTTGGTGGG‑A‑‑g‑g‑g < 1:1384092/35‑1 (MQ=255) tGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AGGG‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa < 2:777936/106‑1 (MQ=255) tGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AGGG‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa > 1:777936/1‑106 (MQ=255) tGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa > 2:1639900/1‑105 (MQ=255) tGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa < 1:1639900/105‑1 (MQ=255) tGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCAt > 1:756559/1‑119 (MQ=255) tGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCAt < 2:756559/119‑1 (MQ=255) gCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa < 1:322171/108‑1 (MQ=255) gCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa > 2:322171/1‑108 (MQ=255) aaTATCAATGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg < 2:629800/139‑1 (MQ=255) aaTATCAACGTGATTTTTGGTGCG‑A‑‑GAG‑GGGGGAACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTATCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg < 1:1749012/139‑1 (MQ=21) tCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc < 2:1764940/95‑1 (MQ=255) tCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc > 1:1764940/1‑95 (MQ=255) cAACGTGGTTTTTGGTGCG‑A‑‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg < 2:1298191/139‑1 (MQ=255) aaCGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg < 2:1810821/139‑1 (MQ=255) gTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg > 1:943505/1‑66 (MQ=255) gTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg < 2:943505/66‑1 (MQ=255) gTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaa > 1:805818/1‑112 (MQ=255) gTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaa < 2:805818/112‑1 (MQ=255) gTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCt > 2:275486/1‑139 (MQ=255) gTTTTTGCTGCGCG‑‑G‑G‑GGGGGGGCTTGGAGCCCCACGTCCGCAAGCACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGTATACCCTCAATTCCTAAAGAGAATTGCTACCACGGCGTAGCATTCt > 1:1302435/1‑139 (MQ=17) tttttGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaa > 1:1432303/1‑111 (MQ=255) tttttGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaa < 2:1432303/111‑1 (MQ=255) tttGGTGCG‑A‑‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACt > 2:315500/1‑47 (MQ=32) tttGGTGCG‑A‑‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACt < 1:315500/47‑1 (MQ=32) tGGTGCG‑A‑‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATAGTATCAATTCTTTAAAAGAATTGCTACCACTGAGGCGGATTGTAGTgac > 1:1298191/1‑137 (MQ=17) | ATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGT > NC_000913/4606187‑4606453 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |