Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,276	G→A	*392* (TAG→TAA)	ubiF →	2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,276	0	G	A	100.0%	172.3 / NA	54	*392* (TAG→TAA)	ubiF	2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/27);  total (27/27)

TTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCA  >  NC_000913/696154‑696399                                                                                                                           |                                                                                                                             ttttATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCg                                                                                                              >  1:1517318/1‑139 (MQ=255)   ttATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGa                                                                                                            >  1:1575303/1‑139 (MQ=255)          ggATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGTGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGc                                                                                                     <  2:514484/139‑1 (MQ=255)           gaatCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGcc                                                                                                    <  2:1040354/136‑1 (MQ=255)           gATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGcc                                                                                                               <  2:961911/128‑1 (MQ=255)           gATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGcc                                                                                                               >  1:961911/1‑128 (MQ=255)           gATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGcc                                                                                                    >  1:1770775/1‑139 (MQ=255)                aGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCg                                                                                               <  2:1575303/139‑1 (MQ=255)                         cTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGa                                                                                     <  1:1036591/139‑1 (MQ=255)                          tGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGAGGCGGAGCGTGCTGGCGTGGTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGAt                                                                                    >  1:1240659/1‑139 (MQ=255)                            ccaccaCTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATaa                                                                                  >  1:585839/1‑139 (MQ=255)                                 acTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGAt                                                                                    >  1:134954/1‑132 (MQ=255)                                 acTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGAt                                                                                    <  2:134954/132‑1 (MQ=255)                                          tATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGcc                                                                                                    >  1:1449013/1‑108 (MQ=255)                                          tATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGcc                                                                                                    <  2:1449013/108‑1 (MQ=255)                                            tGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGc                                                                  >  2:186483/1‑139 (MQ=255)                                             gCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCa                                                                 <  2:1770775/139‑1 (MQ=255)                                               gTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATc                                                               >  1:1694677/1‑139 (MQ=255)                                               gTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATc                                                               <  2:1694677/139‑1 (MQ=255)                                                 aaTCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCg                                                             >  2:1220508/1‑139 (MQ=255)                                                  aTCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGt                                                                                          <  2:794596/110‑1 (MQ=255)                                                  aTCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGt                                                                                          >  1:794596/1‑110 (MQ=255)                                                        ggTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGt                                                                                              <  2:1090179/100‑1 (MQ=255)                                                        ggTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGt                                                                                              >  1:1090179/1‑100 (MQ=255)                                                        ggTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTGa                                                      <  2:896742/139‑1 (MQ=255)                                                                 cggcggAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATcc                                                              <  1:1270822/122‑1 (MQ=255)                                                                 cggcggAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGATTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATcc                                                              >  2:1270822/1‑122 (MQ=255)                                                                   gcggAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGcc                                                                                                               >  1:54690/1‑72 (MQ=255)                                                                   gcggAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGcc                                                                                                               <  2:54690/72‑1 (MQ=255)                                                                   gcggAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACg                                           >  2:1081592/1‑139 (MQ=255)                                                                       aGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACgtg                                         <  2:1320948/137‑1 (MQ=255)                                                                       aGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACgtg                                         >  1:1320948/1‑137 (MQ=255)                                                                           tGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACa                                   <  2:1495903/139‑1 (MQ=255)                                                                           tGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACa                                   <  2:585839/139‑1 (MQ=255)                                                                              tGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCg                                >  1:621990/1‑139 (MQ=255)                                                                                gCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGAt                                                                                                           <  2:793950/63‑1 (MQ=255)                                                                                gCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGAt                                                                                                           >  1:793950/1‑63 (MQ=255)                                                                                   tgtTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCAtt                            >  2:816092/1‑139 (MQ=255)                                                                                   tgtTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTg                                                       <  1:549266/111‑1 (MQ=255)                                                                                   tgtTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTg                                                       >  2:549266/1‑111 (MQ=255)                                                                                   tgtTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTc                           <  1:1220508/139‑1 (MQ=255)                                                                                     ttGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGa                         >  1:1583927/1‑139 (MQ=255)                                                                                          aCGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACAt                      <  2:1853303/137‑1 (MQ=255)                                                                                          aCGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACAt                      >  1:1853303/1‑137 (MQ=255)                                                                                             tCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGAATAAGACGTGTCAACATCGCATTCGACATtgaat                 <  2:621990/139‑1 (MQ=255)                                                                                                ggCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATtgaatgaa              <  2:1655511/139‑1 (MQ=255)                                                                                                      gAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGa        >  1:1836346/1‑139 (MQ=255)                                                                                                         atatGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGaaa      <  1:816092/139‑1 (MQ=255)                                                                                                            tGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCa  >  2:1860934/1‑139 (MQ=255)                                                                                                            tGCGCTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCa  <  2:1240659/139‑1 (MQ=255)                                                                                                               gTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCAtt                            <  1:1436253/110‑1 (MQ=255)                                                                                                               gTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCAtt                            >  2:1436253/1‑110 (MQ=255)                                                                                                                 tAGGATTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTc                                                                    >  1:899918/1‑68 (MQ=255)                                                                                                                 tAGGATTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTc                                                                    <  2:899918/68‑1 (MQ=255)                                                                                                                           |                                                                                                                             TTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTC‑GGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCA  >  NC_000913/696154‑696399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑