Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	183,620	G→A	*386* (TAG→TAA)	cdaR →	carbohydrate diacid regulon transcriptional regulator, autoregulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	183,620	0	G	A	100.0%	244.0 / NA	74	*386* (TAG→TAA)	cdaR	carbohydrate diacid regulon transcriptional regulator, autoregulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/34);  total (40/34)

CAAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCT  >  NC_000913/183484‑183755                                                                                                                                         |                                                                                                                                        cAAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGtt                                                                                                                                      <  2:1495827/139‑1 (MQ=255)                 tCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAg                                                                                                                      >  1:906642/1‑139 (MQ=255)                      gTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAggcggc                                                                                                                 >  1:378346/1‑139 (MQ=255)                      gTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAggcggc                                                                                                                 >  1:1434297/1‑139 (MQ=255)                            cccTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGc                                                                                                           <  2:1434297/139‑1 (MQ=255)                               tGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCa                                                                                                        >  1:1876897/1‑139 (MQ=255)                                 gAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                                <  1:829238/129‑1 (MQ=255)                                 gAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                                >  2:829238/1‑129 (MQ=255)                                 gAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCAt                                                                                                       <  1:260218/138‑1 (MQ=255)                                 gAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCAt                                                                                                       >  2:260218/1‑138 (MQ=255)                                   gTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTcc                                                                                                    >  2:588042/1‑139 (MQ=255)                                       cGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTc                                                                                                >  2:1142108/1‑139 (MQ=255)                                       cGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTc                                                                                                >  1:1716263/1‑139 (MQ=255)                                        ggCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGG‑TGAGGCGGCGCTGGCTCATTCCCCTCAg                                                                                              >  2:1397543/1‑139 (MQ=255)                                             aaTCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                                          <  2:378346/139‑1 (MQ=255)                                              aTCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGG‑TGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                        <  1:1397543/139‑1 (MQ=255)                                               tCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                        <  1:588042/139‑1 (MQ=255)                                                    tatCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTa                                                                                   >  1:941445/1‑139 (MQ=255)                                                     atCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTag                                                                                  <  2:906642/139‑1 (MQ=255)                                                         gAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAggc                                                                                                                    <  1:1755036/101‑1 (MQ=255)                                                         gAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAggc                                                                                                                    >  2:1755036/1‑101 (MQ=255)                                                         gAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                                >  1:1587176/1‑105 (MQ=255)                                                         gAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                                <  2:1587176/105‑1 (MQ=255)                                                         gAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAggg                                                                              >  2:760177/1‑139 (MQ=255)                                                            cTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAggc                                                                                                                    <  2:970147/98‑1 (MQ=255)                                                            cTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAggc                                                                                                                    >  1:970147/1‑98 (MQ=255)                                                               aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                        >  2:1749652/1‑139 (MQ=255)                                                               aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                        >  1:643935/1‑139 (MQ=255)                                                               aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGAGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                        >  1:437442/1‑139 (MQ=255)                                                                ccGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGcc                                                                       <  1:681232/139‑1 (MQ=255)                                                                ccGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGcc                                                                       <  1:760177/139‑1 (MQ=255)                                                                   ggCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGa                                                                    >  1:464112/1‑139 (MQ=255)                                                                         aTTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                                >  2:1879971/1‑89 (MQ=255)                                                                         aTTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                                <  1:1879971/89‑1 (MQ=255)                                                                            tGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGCTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGACGGGTAGAGGGGAATGCCCGAATTAACCGt                                                           <  1:1749652/139‑1 (MQ=255)                                                                              ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGTGTTACAACTGG‑TGAAGAGCGGTAAGtt                                                                                                                                      >  1:188077/1‑62 (MQ=255)                                                                              ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGtt                                                                                                                                      <  2:188077/62‑1 (MQ=255)                                                                              ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGt                                                                                                                                <  1:210287/68‑1 (MQ=255)                                                                              ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGt                                                                                                                                >  2:210287/1‑68 (MQ=255)                                                                              ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGc                                                                                                           <  2:1397457/89‑1 (MQ=255)                                                                              ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGc                                                                                                           >  1:1397457/1‑89 (MQ=255)                                                                              ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCa                                                         >  2:845985/1‑139 (MQ=255)                                                                              ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCa                                                         >  2:1647759/1‑139 (MQ=255)                                                                                 aaTTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGtt                                                      <  1:845985/139‑1 (MQ=255)                                                                                      tgatgaCAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGa                                                 <  2:464112/139‑1 (MQ=255)                                                                                      tgatgaCAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGa                                                 <  2:1876897/139‑1 (MQ=255)                                                                                        atgaCAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAAcc                                                             >  1:1390627/1‑125 (MQ=255)                                                                                        atgaCAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAAcc                                                             <  2:1390627/125‑1 (MQ=255)                                                                                                 tgctgcTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTAcgcg                                      <  2:941445/139‑1 (MQ=255)                                                                                                         tATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                        <  2:1639184/81‑1 (MQ=255)                                                                                                         tATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                        >  1:1639184/1‑81 (MQ=255)                                                                                                         tATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGtttt                              <  1:298476/139‑1 (MQ=255)                                                                                                         tATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGtttt                              >  2:789731/1‑139 (MQ=255)                                                                                                                cGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCg                                                                     <  1:1637899/93‑1 (MQ=255)                                                                                                                cGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCg                                                                     >  2:1637899/1‑93 (MQ=255)                                                                                                                  ttACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCg                     >  1:1743272/1‑139 (MQ=255)                                                                                                                   tACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGa                        >  2:1288327/1‑135 (MQ=255)                                                                                                                   tACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGa                        <  1:1288327/135‑1 (MQ=255)                                                                                                                   tACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCgg                    >  2:453269/1‑139 (MQ=255)                                                                                                                    aCAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAggg                                                                                     >  2:237136/1‑73 (MQ=255)                                                                                                                    aCAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAggg                                                                                     <  1:237136/73‑1 (MQ=255)                                                                                                                     cAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                        >  1:1416309/1‑69 (MQ=255)                                                                                                                     cAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                        <  2:1416309/69‑1 (MQ=255)                                                                                                                     cAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCgg                                                   >  2:982559/1‑106 (MQ=255)                                                                                                                     cAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCgg                                                   <  1:982559/106‑1 (MQ=255)                                                                                                                     cAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCt                  >  2:618599/1‑139 (MQ=255)                                                                                                                      aaCTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCtt                 <  1:453269/139‑1 (MQ=255)                                                                                                                           ggtGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAAt             >  1:141834/3‑139 (MQ=255)                                                                                                                            gtGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGtt                                                      >  2:1008833/2‑97 (MQ=255)                                                                                                                            gtGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGtt                                                      <  1:1008833/96‑1 (MQ=255)                                                                                                                              gAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGt                                 >  2:1056765/1‑116 (MQ=255)                                                                                                                              gAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGt                                 <  1:1056765/116‑1 (MQ=255)                                                                                                                               aaGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCg         <  2:643935/139‑1 (MQ=255)                                                                                                                                      gTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCt  >  1:1351358/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                        CAAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCT  >  NC_000913/183484‑183755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑