Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,800,475	G→A	*110* (TAG→TAA)	ygaM →	putative membrane‑anchored DUF883 family ribosome‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,800,475	0	G	A	100.0%	205.6 / NA	63	*110* (TAG→TAA)	ygaM	putative membrane‑anchored DUF883 family ribosome‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/35);  total (28/35)

GTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATA  >  NC_000913/2800344‑2800610                                                                                                                                    |                                                                                                                                         gTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCaaaa                                                                                                                                   >  2:1293125/1‑139 (MQ=255)   ccAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAAt                                                                                                                                 <  2:1613729/139‑1 (MQ=255)                        cGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTg                                                                                                            <  2:673586/139‑1 (MQ=255)                                 cgcgGATTCTTTTGTCCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAg                                                                                                  >  1:1253723/1‑139 (MQ=255)                                    ggATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCt                                                                                               <  1:1819207/139‑1 (MQ=255)                                           tttGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCg                                                                                        <  1:1293125/139‑1 (MQ=255)                                           tttGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCg                                                                                        <  2:1882218/139‑1 (MQ=255)                                               ttCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGt                                                                                    <  2:1501995/139‑1 (MQ=255)                                                   tGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGa                                                                                                                               >  1:617762/1‑93 (MQ=255)                                                   tGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGa                                                                                                                               <  2:617762/93‑1 (MQ=255)                                                                     cGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATcac                                                              >  1:1140969/1‑139 (MQ=255)                                                                          gTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGa                                                                                                    <  1:1452376/96‑1 (MQ=255)                                                                          gTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGa                                                                                                    >  2:1452376/1‑96 (MQ=255)                                                                          gTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaaaa                                                         >  1:1347872/1‑139 (MQ=255)                                                                              agcagcTGCGGTAGGTATTTTTATCGGTGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATccc                                                     >  2:1058095/1‑139 (MQ=255)                                                                               gcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATcccc                                                    <  2:87182/139‑1 (MQ=255)                                                                                  gcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATCAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCAC‑AAAATCCCCCTAc                                                <  2:1140969/139‑1 (MQ=255)                                                                                     gCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGa                                                                                                    <  1:451622/85‑1 (MQ=255)                                                                                     gCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGa                                                                                                    >  2:451622/1‑85 (MQ=255)                                                                                      cGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAcccc                                             >  2:462772/1‑139 (MQ=255)                                                                                         taggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTc                                          <  2:1620942/139‑1 (MQ=255)                                                                                           ggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCtt                                                                                                              <  1:560164/70‑1 (MQ=255)                                                                                           ggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCtt                                                                                                              >  2:560164/1‑70 (MQ=255)                                                                                            gtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaaa                                                          >  2:1547428/1‑120 (MQ=255)                                                                                            gtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaaa                                                          <  1:1547428/120‑1 (MQ=255)                                                                                                ttttATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAcc                                               <  1:25807/127‑1 (MQ=255)                                                                                                ttttATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAcc                                               >  2:25807/1‑127 (MQ=255)                                                                                                ttttATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCa                                   >  2:425254/1‑139 (MQ=255)                                                                                                   tATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCatat                                <  1:462772/139‑1 (MQ=255)                                                                                                    aTCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATc                               >  1:1436561/1‑139 (MQ=255)                                                                                                      cGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGcc                                                                                                <  1:1632743/72‑1 (MQ=255)                                                                                                      cGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGcc                                                                                                >  2:1632743/1‑72 (MQ=255)                                                                                                       ggCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAg                            >  2:432919/1‑139 (MQ=255)                                                                                                         cgcACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAggg                          >  1:611798/1‑139 (MQ=255)                                                                                                                  aaGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGa                 >  2:1774956/1‑139 (MQ=255)                                                                                                                   aGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCCTATCCAGGGTAATTTCGAc                <  2:1253723/139‑1 (MQ=255)                                                                                                                   aGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc                >  1:554648/1‑139 (MQ=255)                                                                                                                   aGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc                >  1:1224691/1‑139 (MQ=255)                                                                                                                    gCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAcc               <  2:1436561/139‑1 (MQ=255)                                                                                                                       tGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACt            <  1:1058095/139‑1 (MQ=255)                                                                                                                        gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCttt                                                                              >  2:962824/1‑72 (MQ=255)                                                                                                                        gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCttt                                                                              <  1:962824/72‑1 (MQ=255)                                                                                                                        gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaa                                                           >  1:673049/1‑91 (MQ=255)                                                                                                                        gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaa                                                           <  2:673049/91‑1 (MQ=255)                                                                                                                        gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAcc                                               <  2:168766/103‑1 (MQ=255)                                                                                                                        gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAcc                                               >  1:168766/1‑103 (MQ=255)                                                                                                                        gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGt                                           >  2:172382/1‑107 (MQ=255)                                                                                                                        gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGt                                           <  1:172382/107‑1 (MQ=255)                                                                                                                        gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc                >  2:1425055/1‑134 (MQ=255)                                                                                                                        gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc                <  1:1425055/134‑1 (MQ=255)                                                                                                                        gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTa           >  1:947573/1‑139 (MQ=255)                                                                                                                        gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTa           >  1:809602/1‑139 (MQ=255)                                                                                                                         cgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt          <  1:302632/139‑1 (MQ=255)                                                                                                                         cgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt          <  2:947573/139‑1 (MQ=255)                                                                                                                         cgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt          <  1:425254/139‑1 (MQ=255)                                                                                                                          gcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt          <  2:850290/139‑1 (MQ=255)                                                                                                                          gcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACg                                       <  1:1854377/109‑1 (MQ=255)                                                                                                                          gcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACg                                       >  2:1854377/1‑109 (MQ=255)                                                                                                                           cAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAttt        <  2:611798/139‑1 (MQ=255)                                                                                                                             aaTCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCTACCACTATTTGc      <  1:432919/139‑1 (MQ=255)                                                                                                                             aaTCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGc      <  2:809602/139‑1 (MQ=255)                                                                                                                              aTCGTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCt     <  1:1774956/139‑1 (MQ=255)                                                                                                                                 gTAATGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCtata  <  2:554648/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         GTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGA‑CCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATA  >  NC_000913/2800344‑2800610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑