Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,544,074	C→T	*257* (TAG→TAA)	bioH ←	pimeloyl‑ACP methyl ester carboxylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,544,074	0	C	T	100.0%	188.7 / NA	58	*257* (TAG→TAA)	bioH	pimeloyl‑ACP methyl ester carboxylesterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/29);  total (29/29)

AGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTT  >  NC_000913/3543940‑3544207                                                                                                                                        |                                                                                                                                      aGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGTCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTaca                                                                                                                                   <  1:141895/139‑1 (MQ=255) aGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTaca                                                                                                                                   <  2:120817/139‑1 (MQ=255) aGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTaca                                                                                                                                   <  2:823506/139‑1 (MQ=255)      tGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCctc                                                                                                                              >  1:1159936/1‑139 (MQ=255)      tGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCctc                                                                                                                              >  2:1281576/1‑139 (MQ=255)      tGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCctc                                                                                                                              >  1:410671/1‑139 (MQ=255)             aGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCa                                                                                                                       >  1:1475868/1‑139 (MQ=255)               ccAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAAc                                                                                                                     <  2:1778606/139‑1 (MQ=255)                         gCTTGAATCCGGAGTTCCTCTTGTAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACcagca                                                                                                           <  1:457832/139‑1 (MQ=255)                          cTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAGG‑CCACTTACACCCTCTGCTTCAACGCCACcagcag                                                                                                          >  2:1134562/1‑139 (MQ=255)                             gAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTg                                                                                                       >  1:1008688/1‑139 (MQ=255)                              aaTCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGa                                                                                                      >  1:1521419/1‑139 (MQ=255)                               aTCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACAcc                                                                                                                                 <  2:1615064/111‑1 (MQ=255)                               aTCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACAcc                                                                                                                                 >  1:1615064/1‑111 (MQ=255)                                          ctctTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGt                                                                                                        <  1:358001/125‑1 (MQ=255)                                          ctctTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGt                                                                                                        >  2:358001/1‑125 (MQ=255)                                                cAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGc                                                                                    <  2:1475868/139‑1 (MQ=255)                                                       catGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACg                                                                                                                    >  2:809054/1‑100 (MQ=255)                                                       catGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACg                                                                                                                    <  1:809054/100‑1 (MQ=255)                                                         tGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACcagcag                                                                                                          <  2:157234/108‑1 (MQ=255)                                                         tGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACcagcag                                                                                                          >  1:157234/1‑108 (MQ=255)                                                         tGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaataaat                                                                           >  1:653037/1‑139 (MQ=255)                                                             cTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGgcg                                                                       >  1:317334/1‑139 (MQ=255)                                                               ttACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTaca                                                                                                                                   >  2:1539965/1‑77 (MQ=255)                                                               ttACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTaca                                                                                                                                   <  1:1539965/77‑1 (MQ=255)                                                               ttACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACg                                                                                                                    <  2:936548/92‑1 (MQ=255)                                                               ttACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACg                                                                                                                    >  1:936548/1‑92 (MQ=255)                                                                     ggCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGcc                                                               >  1:849157/1‑139 (MQ=255)                                                                      gCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCg                                                              >  1:1371837/1‑139 (MQ=255)                                                                        tGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGcc                                                                                          <  1:1774058/109‑1 (MQ=255)                                                                        tGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGcc                                                                                          >  2:1774058/1‑109 (MQ=255)                                                                        tGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCt                                                            <  2:1371837/139‑1 (MQ=255)                                                                            agaagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTgg                                                        >  1:1234162/1‑139 (MQ=255)                                                                              aagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCTACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                      <  1:1281576/139‑1 (MQ=255)                                                                              aagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                      <  2:1234162/139‑1 (MQ=255)                                                                              aagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                      <  2:317334/139‑1 (MQ=255)                                                                              aagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                      <  2:653037/139‑1 (MQ=255)                                                                                    agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTgg                                                        >  1:62184/1‑131 (MQ=255)                                                                                    agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTgg                                                        <  2:62184/131‑1 (MQ=255)                                                                                    agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                      <  2:937196/133‑1 (MQ=255)                                                                                    agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                      >  1:937196/1‑133 (MQ=255)                                                                                       tGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGatatat                                             <  1:1134562/139‑1 (MQ=255)                                                                                       tGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGatatat                                             <  2:849157/139‑1 (MQ=255)                                                                                        ggCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                      <  2:23412/129‑1 (MQ=255)                                                                                        ggCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                      >  1:23412/1‑129 (MQ=255)                                                                                             cggcgAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGAt                                                                                      >  2:1754934/1‑92 (MQ=255)                                                                                             cggcgAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGAt                                                                                      <  1:1754934/92‑1 (MQ=255)                                                                                              ggcgAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGAt                                                                                      >  1:730720/1‑91 (MQ=255)                                                                                              ggcgAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGAt                                                                                      <  2:730720/91‑1 (MQ=255)                                                                                                cgAGTCAGTGAAGTTCTGTCTCGCCATTTCAAAAGCCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCa                                                                     >  2:1260322/1‑107 (MQ=255)                                                                                                cgAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGTAATAAATGGCGCa                                                                     <  1:1260322/107‑1 (MQ=255)                                                                                                cgAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCtg                                    >  1:1551316/1‑139 (MQ=255)                                                                                                               cTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaat                                                                               >  1:268839/1‑81 (MQ=255)                                                                                                               cTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaat                                                                               <  2:268839/81‑1 (MQ=255)                                                                                                               cTGTCTCGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTa                     >  1:484632/1‑139 (MQ=255)                                                                                                                  tctcGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                      <  1:1612599/103‑1 (MQ=255)                                                                                                                  tctcGCCATTTCAAAAG‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                      >  2:1612599/1‑103 (MQ=255)                                                                                                                                  g‑CCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACtt  <  2:484632/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      AGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAG‑CCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTT  >  NC_000913/3543940‑3544207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑