Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,214,339	(C)6→5	coding (306/1686 nt)	yehU ←	inner membrane putative sensory kinase in two‑component system with YehT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,214,334	0	C	.	100.0%	116.0 / NA	28	coding (311/1686 nt)	yehU	inner membrane putative sensory kinase in two‑component system with YehT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (14/14);  total (14/14)

GACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAC  >  NC_000913/2214198‑2214468                                                                                                                                         |                                                                                                                                       gacgaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                      <  1:234333/139‑1 (MQ=255)    gaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccccca                                                                                                                                   <  1:378091/139‑2 (MQ=255)                                 tAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGc                                                                                                     >  1:425132/1‑139 (MQ=255)                                  aaaGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGcc                                                                                                    >  1:809661/1‑139 (MQ=255)                                      aCTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                                <  2:809661/139‑1 (MQ=255)                                      aCTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                                <  2:425132/139‑1 (MQ=255)                                         ttATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAg                                                                                             >  2:869641/1‑139 (MQ=255)                                                tGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcg                                                                                      >  1:1017647/1‑139 (MQ=255)                                                     ccGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCgacgac                                                                                 >  1:274886/1‑139 (MQ=255)                                                         ggcggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccgga                                                                             <  1:476930/139‑1 (MQ=255)                                                            ggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccggaccg                                                                          <  2:274886/139‑1 (MQ=255)                                                             gATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGGTCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGAccgc                                                                         >  2:748447/1‑139 (MQ=255)                                                                     tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 <  2:1017647/139‑1 (MQ=255)                                                                       cTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                                <  2:802293/106‑1 (MQ=255)                                                                       cTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                                >  1:802293/1‑106 (MQ=255)                                                                          tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccgcc                                                            <  1:171024/139‑1 (MQ=255)                                                                            tACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCa                                                          >  1:1012547/1‑139 (MQ=255)                                                                               cAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 >  1:827066/1‑129 (MQ=255)                                                                               cAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 <  2:827066/129‑1 (MQ=255)                                                                                         aGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTa                                                                   <  2:1169242/117‑1 (MQ=255)                                                                                         aGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTa                                                                   >  1:1169242/1‑117 (MQ=255)                                                                                             aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  1:971485/139‑1 (MQ=255)                                                                                             aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  2:201893/139‑1 (MQ=255)                                                                                                   cAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGcccccatc                                                                                                                                 <  2:127304/46‑4 (MQ=255)                                                                                                   cAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGcccccatc                                                                                                                                 >  1:127304/1‑43 (MQ=255)                                                                                                         tGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                                >  1:535125/1‑72 (MQ=255)                                                                                                         tGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                                <  2:535125/72‑1 (MQ=255)                                                                                                            tCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                                <  2:994090/69‑1 (MQ=255)                                                                                                            tCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                                >  1:994090/1‑69 (MQ=255)                                                                                                                gaTCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAAt                      <  2:1012547/139‑1 (MQ=255)                                                                                                                                    tCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAc  >  2:558725/1‑139 (MQ=255)                                                                                                                                    tCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAc  >  1:931618/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                       GACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAC  >  NC_000913/2214198‑2214468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑