Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,309,006	(C)6→5	intergenic (‑82/+27)	nlpI ← / ← pnp	lipoprotein involved in osmotic sensitivity and filamentation/polynucleotide phosphorylase/polyadenylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,309,001	0	C	.	100.0%	212.0 / NA	50	intergenic (‑77/+32)	nlpI/pnp	lipoprotein involved in osmotic sensitivity and filamentation/polynucleotide phosphorylase/polyadenylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (21/29);  total (21/29)

CATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGA  >  NC_000913/3308869‑3309129                                                                                                                                     |                                                                                                                                 cATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcccccac                                                                                                                            <  1:581819/139‑3 (MQ=255)   tCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcccc                                                                                                                               >  1:1030794/1‑134 (MQ=255)   tCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcccc                                                                                                                               <  2:1030794/134‑1 (MQ=255)   tCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGATGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcccccaccg                                                                                                                          >  2:928483/1‑135 (MQ=255)   tCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGATTGGATGAACGTCCTGTGCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcccccaccg                                                                                                                          >  2:831455/1‑135 (MQ=255)   tCCTGCAAGCGTAAATGCTGTCTCAACGAAACACCAGCCCAAAAAAGGCTTCATTTCCCACTCCCGCACACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGCCCTGCCCGGTTAAAAccccccaccg                                                                                                                          >  2:464137/1‑135 (MQ=255)      tGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcccccaccgcag                                                                                                                       >  1:756811/1‑132 (MQ=255)      tGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcccccaccgcag                                                                                                                       >  1:457296/1‑132 (MQ=255)      tGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcccccaccgcag                                                                                                                       >  2:463612/1‑132 (MQ=255)               aaGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCa                                                                                                             <  2:756811/139‑1 (MQ=255)                   gCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATg                                                                                                         <  1:928483/139‑1 (MQ=255)                     tGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGc                                                                                                       >  1:1044874/1‑139 (MQ=255)                       tCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCaa                                                                                                     <  2:525164/139‑1 (MQ=255)                           aaCGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCtt                                                                                                 >  2:613371/1‑139 (MQ=255)                                   acCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGccc                                                                                         <  1:64157/139‑1 (MQ=255)                                        cgcAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTc                                                                                    >  1:1233815/1‑139 (MQ=255)                                          cAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTcag                                                                                  >  2:894829/1‑139 (MQ=255)                                                aGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGACCGTCCTGTATCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCg                                                                            <  2:119806/139‑1 (MQ=255)                                                aGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCg                                                                            <  2:541959/139‑1 (MQ=255)                                                       aTTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTc                                                                     <  1:613371/139‑1 (MQ=255)                                                          tCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCgg                                                                  <  2:1044874/139‑1 (MQ=255)                                                            ccACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGtg                                                                <  2:57844/139‑1 (MQ=255)                                                                  ccGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                          <  1:894829/139‑1 (MQ=255)                                                                  ccGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                          <  2:616374/139‑1 (MQ=255)                                                                  ccGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                          <  2:1233815/139‑1 (MQ=255)                                                                  ccGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                          <  1:463612/139‑1 (MQ=255)                                                                  ccGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                          >  1:92248/1‑139 (MQ=255)                                                                          cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCgg                                                                                                                   <  1:514528/74‑1 (MQ=255)                                                                          cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCgg                                                                                                                   >  2:514528/1‑74 (MQ=255)                                                                          cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                          >  1:475396/1‑131 (MQ=255)                                                                          cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                          <  2:475396/131‑1 (MQ=255)                                                                          cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTga                                                  >  1:608083/1‑139 (MQ=255)                                                                            cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAg                                                                                                                 >  2:1118017/1‑74 (MQ=255)                                                                            cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAg                                                                                                                 <  1:1118017/74‑1 (MQ=255)                                                                            cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATg                                                                                                         <  2:1138986/82‑1 (MQ=255)                                                                            cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATg                                                                                                         >  1:1138986/1‑82 (MQ=255)                                                                            cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                          >  2:43928/1‑129 (MQ=255)                                                                            cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                          <  1:43928/129‑1 (MQ=255)                                                                            cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTgaga                                                <  1:1181365/139‑1 (MQ=255)                                                                            cGGTTGAATGAACGTCCTGTACCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTgaga                                                >  1:1018378/1‑139 (MQ=255)                                                                                     gaaCGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGt                                       <  1:831455/139‑1 (MQ=255)                                                                                         gTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGct                                   >  2:774337/1‑139 (MQ=255)                                                                                          tCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGctt                                  <  2:92248/139‑1 (MQ=255)                                                                                          tCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGctt                                  <  2:120435/139‑1 (MQ=255)                                                                                            cTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGcttct                                >  2:228971/1‑139 (MQ=255)                                                                                                    ggTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCt                        <  1:574108/139‑1 (MQ=255)                                                                                                     gTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTc                       >  2:504019/1‑139 (MQ=255)                                                                                                        gCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGa                    <  2:457296/139‑1 (MQ=255)                                                                                                         cTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                          >  2:1070303/1‑100 (MQ=255)                                                                                                         cTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                          <  2:442057/100‑1 (MQ=255)                                                                                                         cTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                          >  1:442057/1‑100 (MQ=255)                                                                                                         cTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                          <  1:1070303/100‑1 (MQ=255)                                                                                                                ggCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTgaga                                                >  2:560311/1‑103 (MQ=255)                                                                                                                ggCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTgaga                                                <  1:560311/103‑1 (MQ=255)                                                                                                                    tCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCg                                                                                            >  2:936525/1‑55 (MQ=255)                                                                                                                    tCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGccc        >  2:85263/1‑139 (MQ=255)                                                                                                                    tCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGccc        <  1:228971/139‑1 (MQ=255)                                                                                                                    tCCGGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCg                                                                                            <  1:936525/55‑1 (MQ=255)                                                                                                                          ccGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGa  >  2:1152568/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                 CATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGA  >  NC_000913/3308869‑3309129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑