| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,101,039 | (C)7→8 | coding (585/720 nt) | yggN ← | DUF2884 family putative periplasmic protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,101,032 | 1 | . | C | 96.7% | 207.9 / ‑0.6 | 60 | coding (592/720 nt) | yggN | DUF2884 family putative periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (26/32); total (26/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGCG > NC_000913/3100900‑3101165 | agGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑cccccc > 1:246718/1‑139 (MQ=255)agGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑cccccc > 2:159294/1‑139 (MQ=255)agGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑cccccc > 1:181704/1‑139 (MQ=255) tattTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccccccccagg < 1:778465/139‑5 (MQ=255) tattTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccccccccagg < 1:159294/139‑5 (MQ=255) aaTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCa > 1:623191/1‑139 (MQ=255) ttCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGc < 2:623191/139‑1 (MQ=255) ttCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGc < 1:745599/139‑1 (MQ=255) tCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGca < 2:181704/139‑1 (MQ=255) tCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTCCCCAGca < 2:1152441/139‑1 (MQ=255) ccGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGcaca > 1:1006219/1‑139 (MQ=255) gACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACAtt < 2:1054833/139‑1 (MQ=255) tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGt > 1:611464/1‑139 (MQ=255) tCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg < 2:634024/139‑1 (MQ=255) tCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACGGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGG‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg < 2:735959/139‑1 (MQ=255) acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCAc > 1:464420/1‑139 (MQ=255) acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCAcc > 2:1042330/1‑139 (MQ=255) acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCg < 2:391239/139‑1 (MQ=255) cgGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGcac < 1:1170345/99‑1 (MQ=255) cgGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGcac > 2:1170345/1‑99 (MQ=255) gCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTc < 2:997179/90‑1 (MQ=255) gCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTc > 1:997179/1‑90 (MQ=255) aCAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGcc < 1:1002473/139‑1 (MQ=255) cAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGccc > 1:1034765/1‑139 (MQ=255) aaCATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt < 2:1034765/139‑1 (MQ=255) cTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAt > 2:608783/1‑139 (MQ=255) tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGca > 1:1084230/1‑82 (MQ=255) tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGca < 2:1084230/82‑1 (MQ=255) tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑c > 2:347131/1‑62 (MQ=255) tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑c > 2:388103/1‑62 (MQ=255) tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑c < 1:347131/62‑1 (MQ=255) tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑c < 1:388103/62‑1 (MQ=255) gCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAAt > 1:844288/1‑139 (MQ=255) ccAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGc < 2:997280/79‑1 (MQ=255) ccAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGc > 1:997280/1‑79 (MQ=255) ccAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg < 2:468843/139‑1 (MQ=255) ccAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccccccccagg > 1:1286883/1‑66 (MQ=255) ccAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccccccccagg < 2:1286883/70‑5 (MQ=255) aaCTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTg > 2:436292/1‑139 (MQ=255) aCTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGt > 1:1182598/1‑139 (MQ=255) ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg < 1:937863/95‑1 (MQ=255) ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg > 2:937863/1‑95 (MQ=255) cTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGTCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTc < 2:246718/139‑1 (MQ=255) cTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTc < 2:611464/139‑1 (MQ=255) cTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTc < 1:1286921/139‑1 (MQ=255) cTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTc < 2:844288/139‑1 (MQ=255) ttttCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTg > 2:462841/1‑124 (MQ=255) ttttCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTg < 1:462841/124‑1 (MQ=255) ttttCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCa < 1:1042330/139‑1 (MQ=255) tGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCAtt < 2:1182598/139‑1 (MQ=255) gCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTa > 1:19581/1‑125 (MQ=255) gCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTa < 2:19581/125‑1 (MQ=255) cTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGca < 2:1201013/54‑1 (MQ=255) cTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGca > 1:1201013/1‑54 (MQ=255) cTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGCCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGc > 2:240901/1‑139 (MQ=255) tCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGAt < 2:1006219/139‑1 (MQ=255) tttGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCa > 1:802505/1‑37 (MQ=255) tttGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCa < 2:802505/37‑1 (MQ=255) tttGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCa < 2:890321/121‑1 (MQ=255) tttGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCa > 1:890321/1‑121 (MQ=255) gAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGAtt > 2:346060/1‑123 (MQ=255) gAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGAtt < 1:346060/123‑1 (MQ=255) gAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACt < 1:741780/127‑1 (MQ=255) gAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACt > 2:741780/1‑127 (MQ=255) aGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCAcc < 1:55468/66‑1 (MQ=255) aGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCAcc > 2:55468/1‑66 (MQ=255) ggATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACt > 2:559573/1‑125 (MQ=255) ggATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACt < 1:559573/125‑1 (MQ=255) tGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCa > 1:371594/1‑103 (MQ=255) tGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCa < 2:371594/103‑1 (MQ=255) tGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGgcg < 1:509918/139‑1 (MQ=255) | AGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGCG > NC_000913/3100900‑3101165 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |