| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,379,266 | (C)7→8 | coding (732/1128 nt) | zapE ← | divisome ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,379,259 | 1 | . | C | 96.8% | 218.9 / ‑0.4 | 63 | coding (739/1128 nt) | zapE | divisome ATPase |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (28/33); total (29/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
GTGAGAGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTACATCA > NC_000913/3379121‑3379392 | gTGAGAGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGttt < 2:786673/139‑1 (MQ=255) tGAGAGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑‑c > 1:829415/1‑139 (MQ=255) agagCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑‑ccc < 2:398868/139‑1 (MQ=255) aGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAgcg > 2:375999/1‑139 (MQ=255) gTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAgcgc < 1:1261738/139‑1 (MQ=255) tGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAgcgc < 2:389357/135‑1 (MQ=255) tGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAgcgc > 1:389357/1‑135 (MQ=255) tGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAAc > 1:224143/1‑139 (MQ=255) tGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAAc > 1:2782/1‑139 (MQ=255) gCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAAcc < 2:1190991/139‑1 (MQ=255) cTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCa < 1:375999/139‑1 (MQ=255) gACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑‑CCCCCCCGCCAGCGCCAACCATAg > 1:1134648/1‑139 (MQ=255) tgagggCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCCACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAAcc > 2:749926/4‑133 (MQ=255) tGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAAcc < 1:749926/133‑1 (MQ=255) tGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGtt > 2:835578/1‑139 (MQ=255) tGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGtt > 1:538072/1‑139 (MQ=255) tCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCAtt > 2:565587/1‑139 (MQ=255) cGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCGGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTg < 2:1134648/139‑1 (MQ=255) gACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTg > 2:720404/1‑139 (MQ=255) cGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTGTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCGCGGGt > 1:804385/1‑139 (MQ=255) tagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGttt < 1:835578/139‑1 (MQ=255) tagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGttt < 2:829415/139‑1 (MQ=255) gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGttt < 1:508098/89‑1 (MQ=255) gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGttt > 2:508098/1‑89 (MQ=255) gagaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGa > 1:4573/1‑139 (MQ=255) gagaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGa > 1:610778/1‑139 (MQ=255) gaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTTATTTCTAACGTCGGTGAATTTTCTCGttt < 1:621295/81‑1 (MQ=255) gaCAGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGttt > 2:621295/1‑81 (MQ=255) ccAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTgc > 1:604948/1‑114 (MQ=255) ccAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTgc < 2:604948/114‑1 (MQ=255) cAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCACCGTGAAGTGGCGa < 1:565587/139‑1 (MQ=255) tctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGAt < 2:538072/139‑1 (MQ=255) tcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTAATCGTGAAGTGGCGAAAGCCACAGATgc > 1:811658/1‑139 (MQ=255) gACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGaa < 2:567729/117‑1 (MQ=255) gACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGaa > 1:567729/1‑117 (MQ=255) gACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcgc > 1:726102/1‑139 (MQ=255) gACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCAAAAGCCACAGATgcgc < 2:804385/139‑1 (MQ=255) ccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag < 1:1178537/139‑1 (MQ=255) aTTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtg < 2:4573/139‑1 (MQ=255) gttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTgagtgagt < 2:610778/139‑1 (MQ=255) cTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGcc < 2:555445/63‑1 (MQ=255) cTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGcc > 1:555445/1‑63 (MQ=255) ggCCGATGGTTGGTTTCTAAGGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATGGTTTATCCATTTGCGCCCGGGTTTCAGCGGGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGa < 2:764124/139‑1 (MQ=255) ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGttt > 2:1106468/1‑41 (MQ=255) ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGttt < 1:1106468/41‑1 (MQ=255) ccGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACg > 1:566219/1‑139 (MQ=255) ccGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTCTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACg > 2:883432/1‑139 (MQ=255) cGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGa < 2:1018528/139‑1 (MQ=255) aTGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAAc < 1:1213700/56‑1 (MQ=255) aTGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAAc > 2:1213700/1‑56 (MQ=255) aTGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATa > 2:1182210/1‑139 (MQ=255) ggTTGATTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAAt < 2:1178197/139‑1 (MQ=255) aTTTCTAACGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAaca < 2:230635/139‑1 (MQ=255) aCGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAgcgc < 2:424358/38‑1 (MQ=255) aCGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAgcgc > 1:424358/1‑38 (MQ=255) aCGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAgc < 2:180287/36‑1 (MQ=255) aCGTCGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAgc > 1:180287/1‑36 (MQ=255) cGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGcc < 2:138372/35‑1 (MQ=37) cGGTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGcc > 1:138372/1‑35 (MQ=37) ggTGAATTTTCTCGTTTCCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTgcgc < 2:1239245/58‑1 (MQ=255) ggTGAATTTTCTCGTTTCCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTgcgc > 1:1239245/1‑58 (MQ=255) ggTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACg > 2:1034348/1‑111 (MQ=255) ggTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACg < 1:1034348/111‑1 (MQ=255) ggTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACg < 2:726102/139‑1 (MQ=255) ggTGAATTTTCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACg < 2:224143/139‑1 (MQ=255) tttCTCGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTa < 1:1182210/139‑1 (MQ=255) tctcGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACg > 2:1161921/1‑130 (MQ=255) tctcGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACg < 1:1161921/130‑1 (MQ=255) tcGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc < 1:267696/101‑1 (MQ=255) tcGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc > 2:267696/1‑101 (MQ=255) cGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTACATca < 1:720404/139‑1 (MQ=255) cGTTTC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTACATca < 1:883432/139‑1 (MQ=255) | GTGAGAGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTACATCA > NC_000913/3379121‑3379392 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |