| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,609,336 | (C)7→8 | intergenic (+13/‑78) | yjjG → / → prfC | dUMP phosphatase/peptide chain release factor RF‑3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,609,329 | 1 | . | C | 95.7% | 74.8 / ‑3.3 | 23 | intergenic (+6/‑85) | yjjG/prfC | dUMP phosphatase/peptide chain release factor RF‑3 |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (12/10); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACTT > NC_000913/4609192‑4609459 | ctcGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑c < 1:480833/139‑1 (MQ=255) tGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑cccccc < 2:804715/139‑1 (MQ=255) cGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCC‑CGTTGATg < 1:298466/139‑1 (MQ=255) gCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAg > 1:1226661/1‑139 (MQ=255) tCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑cc > 1:985402/1‑90 (MQ=255) tCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCCCCTGTGTAAACACTGATTGCCT‑cc < 2:985402/90‑1 (MQ=255) aGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑cccccc‑ccgttg > 1:498897/1‑90 (MQ=255) aGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑cccccc‑ccgttg < 2:498897/95‑6 (MQ=255) gCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcggcgc > 2:114132/1‑139 (MQ=255) tCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑cccc < 1:586111/72‑1 (MQ=255) tCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑cccc > 2:586111/1‑72 (MQ=255) gACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCt < 2:749759/121‑1 (MQ=255) gACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCt > 1:749759/1‑121 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGa > 2:467217/1‑139 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑cccccc‑ccgttg > 1:1125717/1‑57 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑cccccc‑ccgttg < 2:1125717/62‑6 (MQ=255) ttcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcggcg > 2:634431/1‑105 (MQ=255) ttcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcggcg < 1:634431/105‑1 (MQ=255) tcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑CCCCCCACGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg < 2:591110/139‑1 (MQ=255) tcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCg < 2:614797/83‑1 (MQ=255) tcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCg > 1:614797/1‑83 (MQ=255) gCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑cccccc‑ccgttg > 1:1230653/1‑47 (MQ=255) gCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑cccccc‑ccgttg < 2:1230653/52‑6 (MQ=255) ggAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCa < 1:114132/139‑1 (MQ=255) agcagcTCCTGTGTAAACACTGATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAag > 2:1121827/1‑139 (MQ=255) agcagcTCCTGTGTAAACACTGATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAag < 1:1121827/139‑1 (MQ=255) cTCCTGTGTAAACACTGATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga > 2:1167751/1‑102 (MQ=255) cTCCTGTGTAAACACTGATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga < 1:1167751/102‑1 (MQ=255) aaCACTGATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGaa > 2:335563/1‑134 (MQ=255) aaCACTGATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGaa < 1:335563/134‑1 (MQ=255) acacTGATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGcgccg < 1:63700/139‑1 (MQ=255) acacTGATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGcgccg > 1:613924/1‑139 (MQ=255) tGATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGTGAAGCGCCGCACt > 1:1236449/1‑139 (MQ=255) gATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACtt > 2:697604/1‑139 (MQ=255) gATTGCCTCCCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACtt < 2:805667/139‑1 (MQ=255) | CTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACTT > NC_000913/4609192‑4609459 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |