Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,266,013	T→C	R188R (CGT→CGC)	yeiP →	elongation factor P‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,266,013	0	T	C	100.0%	135.9 / NA	38	R188R (CGT→CGC)	yeiP	elongation factor P‑like protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/19);  total (19/19)

CAAAGGGGCATCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCT  >  NC_000913/2265881‑2266147                                                                                                                                      |                                                                                                                                       cAAAGGGGCATCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGAc                                                                                                                                  >  2:139522/1‑139 (MQ=255)           tCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGc                                                                                                                        >  1:584076/1‑139 (MQ=255)              gCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGTATACTTAAGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGc                                                                                                                     >  2:530115/1‑139 (MQ=255)                  gcgcCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGc                                                                                                                 <  2:945739/139‑1 (MQ=255)                      ccGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGaa                                                                                                             <  1:384625/139‑1 (MQ=255)                           aCAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                        <  1:640111/139‑1 (MQ=255)                                 cGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGTATACTTAAGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCt                                                                                                  <  1:530115/139‑1 (MQ=255)                                                    tctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTaa                                                                               <  1:139522/139‑1 (MQ=255)                                                       ggtGATTCAGGTACCAGAATACTTAAGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAAttt                                                                            >  1:667887/1‑139 (MQ=255)                                                        gtGATTCAGGTACCAGAATACTTAAGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTaa                                                                               <  2:572936/135‑1 (MQ=255)                                                        gtGATTCAGGTACCAGAATACTTAAGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTaa                                                                               >  1:572936/1‑135 (MQ=255)                                                             tCAGGTACCAGAATACTTAAGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGcc                                                                                                                       <  1:1103414/90‑1 (MQ=255)                                                             tCAGGTACCAGAATACTTAAGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGcc                                                                                                                       >  2:1103414/1‑90 (MQ=255)                                                             tCAGGTACCAGAATACTTAAGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATtata                                                                      >  1:1198242/1‑139 (MQ=255)                                                                  tACCAGAATACTTAAGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAAt                                                                 >  2:267550/1‑139 (MQ=255)                                                                             ttAAGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAgg                                                                                              >  2:156251/1‑99 (MQ=255)                                                                             ttAAGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAgg                                                                                              <  1:156251/99‑1 (MQ=255)                                                                               aaGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACt                                                                                                                                 >  1:501021/1‑62 (MQ=255)                                                                               aaGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACt                                                                                                                                 <  2:501021/62‑1 (MQ=255)                                                                               aaGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATg                                                                                                                  >  2:715554/1‑77 (MQ=255)                                                                               aaGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATg                                                                                                                  <  1:715554/77‑1 (MQ=255)                                                                                  cccGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                        >  2:1225351/1‑84 (MQ=255)                                                                                  cccGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                        <  1:1225351/84‑1 (MQ=255)                                                                                  cccGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAc                                                                     >  1:355930/1‑119 (MQ=255)                                                                                  cccGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAc                                                                     <  2:355930/119‑1 (MQ=255)                                                                                    cGGGCGAAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTc                                                >  2:338458/1‑139 (MQ=255)                                                                                    cGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAc                                                                     >  2:366376/1‑117 (MQ=255)                                                                                    cGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAc                                                                     <  1:366376/117‑1 (MQ=255)                                                                                      ggCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTgcgc                                                   >  1:15043/1‑133 (MQ=255)                                                                                      ggCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTgcgc                                                   <  2:15043/133‑1 (MQ=255)                                                                                       gCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGa                                            <  1:536244/139‑1 (MQ=255)                                                                                        cG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGaa                                           <  2:584076/139‑1 (MQ=255)                                                                                                     tATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGccc                               <  2:59964/139‑1 (MQ=255)                                                                                                                aagaaCGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGc                                                                                                                 <  1:1069649/46‑1 (MQ=255)                                                                                                                aagaaCGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGc                                                                                                                 >  2:1069649/1‑46 (MQ=255)                                                                                                                     cGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGtt               >  1:702513/1‑139 (MQ=255)                                                                                                                                  ggCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCt  >  2:519172/1‑139 (MQ=255)                                                                                                                                  ggCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCt  <  1:996267/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       CAAAGGGGCATCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCG‑AAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCT  >  NC_000913/2265881‑2266147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑