Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,288,561	(C)7→6	pseudogene (354/2525 nt)	yejO ←	pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,288,555	0	C	.	100.0%	229.0 / NA	55	pseudogene (360/2525 nt)	yejO	pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (24/31);  total (24/31)

GACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCG  >  NC_000913/2288420‑2288688                                                                                                                                        |                                                                                                                                      gaCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGcccc                                                                                                                                    <  1:1023572/139‑1 (MQ=255)  aCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGccccc                                                                                                                                   >  2:1273618/1‑139 (MQ=255)         gTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCttt                                                                                                                           <  2:987658/139‑1 (MQ=255)                       cATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAgc                                                                                                             >  1:1046539/1‑139 (MQ=255)                              cgcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGcgcccgcc                                                                                                      >  1:746050/1‑139 (MQ=255)                                cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGcccccca                                                                                                                                 <  1:869740/111‑2 (MQ=255)                                cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGcccccca                                                                                                                                 >  2:869740/1‑110 (MQ=255)                                   ggTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTaa                                                                                                 <  2:154610/139‑1 (MQ=255)                                   ggTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTaa                                                                                                 <  2:46933/139‑1 (MQ=255)                                        ttttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTGACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTg                                                                                            <  1:762349/139‑1 (MQ=255)                                                 cACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTc                                                                                   >  2:1207068/1‑139 (MQ=255)                                                  aCCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCg                                                                                  <  2:746050/139‑1 (MQ=255)                                                      ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                              >  1:888581/1‑139 (MQ=255)                                                          cTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCa                                                                          <  1:1207068/139‑1 (MQ=255)                                                          cTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCa                                                                          <  1:1152914/139‑1 (MQ=255)                                                           tttCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCAt                                                                         <  2:860749/139‑1 (MQ=255)                                                               tGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAAc                                                                     <  1:747766/139‑1 (MQ=255)                                                                     aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  1:1273618/139‑1 (MQ=255)                                                                      ccGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGcgccc                                                                                                         >  1:988258/1‑96 (MQ=255)                                                                      ccGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGcgccc                                                                                                         <  2:988258/96‑1 (MQ=255)                                                                       cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:505783/139‑1 (MQ=255)                                                                       cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  1:816077/139‑1 (MQ=255)                                                                           aatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCCACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               >  2:1054154/1‑133 (MQ=255)                                                                           aatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  1:1054154/133‑1 (MQ=255)                                                                           aatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             >  1:57603/1‑135 (MQ=255)                                                                           aatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:57603/135‑1 (MQ=255)                                                                            atgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCa                                                        <  1:531886/139‑1 (MQ=255)                                                                                aaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCattat                                                    <  1:154264/139‑1 (MQ=255)                                                                                       cccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAg                                                                                                              <  1:187224/74‑1 (MQ=255)                                                                                       cccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAg                                                                                                              >  2:187224/1‑74 (MQ=255)                                                                                        ccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTACCACACCATTATTGAGCGTa                                            >  2:1293569/1‑139 (MQ=255)                                                                                          tctTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGcccc                                                                                                                                    <  1:1029154/50‑1 (MQ=255)                                                                                          tctTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGcccc                                                                                                                                    >  2:1029154/1‑50 (MQ=255)                                                                                              tAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTgg                                      >  2:298316/1‑139 (MQ=255)                                                                                                  cTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTg                                  <  2:1130822/139‑1 (MQ=255)                                                                                                    ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTg                                                                                            <  1:1175948/79‑1 (MQ=255)                                                                                                    ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTg                                                                                            >  1:1337244/1‑79 (MQ=255)                                                                                                    ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTg                                                                                            >  2:1175948/1‑79 (MQ=255)                                                                                                    ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTg                                                                                            <  2:1337244/79‑1 (MQ=255)                                                                                                    ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCa                                                        >  1:1386598/1‑115 (MQ=255)                                                                                                    ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCa                                                        <  2:1386598/115‑1 (MQ=255)                                                                                                      agtagtCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCt                                                                                >  2:509840/1‑89 (MQ=255)                                                                                                      agtagtCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCt                                                                                <  1:509840/89‑1 (MQ=255)                                                                                                          gtCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCa                          >  1:1086133/1‑139 (MQ=255)                                                                                                             ctccCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             >  2:1414778/1‑101 (MQ=255)                                                                                                             ctccCTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  1:1414778/101‑1 (MQ=255)                                                                                                               cccTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:1374390/99‑1 (MQ=255)                                                                                                               cccTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             >  1:1374390/1‑99 (MQ=255)                                                                                                                ccTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGcgcc                                                                                                          <  2:1175168/53‑1 (MQ=39)                                                                                                                ccTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGcgcc                                                                                                          >  1:1175168/1‑53 (MQ=39)                                                                                                                ccTTTTCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCAtt                    >  1:214157/1‑139 (MQ=255)                                                                                                                     tCGATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAAc               <  1:298316/139‑1 (MQ=255)                                                                                                                       gATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACaa             >  2:478656/1‑139 (MQ=255)                                                                                                                       gATAGCGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACaa             >  2:690975/1‑139 (MQ=255)                                                                                                                            cGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTg                                                                  >  2:1224074/1‑81 (MQ=255)                                                                                                                            cGACAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTg                                                                  <  1:1224074/81‑1 (MQ=255)                                                                                                                              aCAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  2:1097581/82‑1 (MQ=255)                                                                                                                              aCAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               >  1:1097581/1‑82 (MQ=255)                                                                                                                               cAAAGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATaa     >  1:920798/1‑139 (MQ=255)                                                                                                                                  aGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:1086133/139‑1 (MQ=255)                                                                                                                                  aGACGG‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTAGTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:690975/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      GACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCG  >  NC_000913/2288420‑2288688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑