Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,982,684	(C)8→7	noncoding (79/87 nt)	leuT →	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,982,677	0	C	.	100.0%	186.7 / NA	45	noncoding (72/87 nt)	leuT	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (20/25);  total (20/25)

TTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAC  >  NC_000913/3982553‑3982809                                                                                                                             |                                                                                                                                     ttGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGcacca                                                                                                                       <  2:1009673/139‑1 (MQ=255)  tGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGcaccac                                                                                                                      >  2:780147/1‑139 (MQ=255)  tGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGcaccac                                                                                                                      <  1:729240/139‑1 (MQ=255)           ttCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTccccccctcg                                                                                                                             >  2:121081/1‑121 (MQ=255)           ttCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTccccccctcg                                                                                                                             <  1:121081/124‑4 (MQ=255)                              cccATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCa                                                                                          >  1:1073902/1‑139 (MQ=255)                              cccATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCa                                                                                          >  2:363652/1‑139 (MQ=255)                               ccATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGcacca                                                                                                                       <  1:293780/109‑1 (MQ=255)                               ccATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGcacca                                                                                                                       >  2:293780/1‑109 (MQ=255)                                        gaggTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaa                                                                                        <  2:762448/128‑1 (MQ=255)                                        gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaa                                                                                        >  1:762448/1‑131 (MQ=255)                                        gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                                                                <  2:1075677/139‑1 (MQ=255)                                           gTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGcacca                                                                                                                       >  2:706227/1‑97 (MQ=255)                                           gTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGcacca                                                                                                                       <  1:706227/97‑1 (MQ=255)                                               tgACAATGCGAAGGTGGCGGAATTGGTAGACTCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGc                                                                         >  2:802144/1‑139 (MQ=255)                                               tgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACt                                                                                                                  >  1:272368/1‑98 (MQ=255)                                               tgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACt                                                                                                                  <  2:272368/98‑1 (MQ=255)                                               tgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCg                                                                           >  2:998081/1‑137 (MQ=255)                                               tgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCg                                                                           <  1:998081/137‑1 (MQ=255)                                               tgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGc                                                                         >  2:130587/1‑139 (MQ=255)                                                gACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCg                                                                        >  2:398172/1‑139 (MQ=255)                                                 aCAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGa                                                                       <  1:675345/139‑1 (MQ=255)                                                     tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAg                                                                   <  2:306532/139‑1 (MQ=255)                                                         aaGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGa                                                                       <  1:910014/131‑1 (MQ=255)                                                         aaGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGa                                                                       >  2:910014/1‑131 (MQ=255)                                                          aGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAg                                                              <  1:780147/139‑1 (MQ=255)                                                             tGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCtt                                                           >  1:594107/1‑139 (MQ=255)                                                               gCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTgg                                                         <  1:924575/139‑1 (MQ=255)                                                                  gAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAg                                                      <  1:363652/139‑1 (MQ=255)                                                                         tAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACt                                               <  2:594107/139‑1 (MQ=255)                                                                                  tAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGa                                      <  2:1073902/139‑1 (MQ=255)                                                                                              ttAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCa                                                                                          >  2:1159075/1‑75 (MQ=255)                                                                                              ttAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCa                                                                                          <  1:1159075/75‑1 (MQ=255)                                                                                               tAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGa                         <  1:802144/139‑1 (MQ=255)                                                                                                   gtCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGtt                     <  1:130587/139‑1 (MQ=255)                                                                                                     ccTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCa                                   <  1:843410/123‑1 (MQ=255)                                                                                                     ccTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCa                                   >  2:843410/1‑123 (MQ=255)                                                                                                      cTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAg                                                                                     <  2:147889/72‑1 (MQ=255)                                                                                                      cTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAg                                                                                     >  1:147889/1‑72 (MQ=255)                                                                                                        tACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTgg                                        <  1:1258697/115‑1 (MQ=255)                                                                                                        tACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTgg                                        >  2:1258697/1‑115 (MQ=255)                                                                                                         aCGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGtt                     <  2:1010543/133‑1 (MQ=255)                                                                                                         aCGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGtt                     >  1:1010543/1‑133 (MQ=255)                                                                                                              cGTGGGGGTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCctctc          >  1:1199237/1‑139 (MQ=255)                                                                                                                 gggggTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGtt                     <  1:861708/125‑1 (MQ=255)                                                                                                                 gggggTTCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGtt                     >  2:861708/1‑125 (MQ=255)                                                                                                                      ttCAAGT‑CCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  <  2:1199237/139‑1 (MQ=255)                                                                                                                             |                                                                                                                                     TTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAC  >  NC_000913/3982553‑3982809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑