| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,734,543 | (C)8→9 | coding (492/732 nt) | yfiH ← | UPF0124 family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,734,535 | 1 | . | C | 98.0% | 176.2 / ‑3.4 | 51 | coding (500/732 nt) | yfiH | UPF0124 family protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (28/22); total (29/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
AAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑T‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGC > NC_000913/2734403‑2734662 | aaaaTTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑T‑‑cccccc < 1:635463/139‑1 (MQ=255) gCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGaa > 2:431696/1‑116 (MQ=255) gCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGaa < 1:431696/116‑1 (MQ=255) gCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAAc < 1:1341848/139‑1 (MQ=255) tAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGcc > 2:67660/1‑125 (MQ=255) tAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGcc < 1:67660/125‑1 (MQ=255) gCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑t < 2:776174/76‑1 (MQ=255) gCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑t > 1:776174/1‑76 (MQ=255) gATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGa < 1:415044/139‑1 (MQ=255) aTCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCa < 2:509780/113‑1 (MQ=255) aTCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCa > 1:509780/1‑113 (MQ=255) aTCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TCCCCCCCCCCACTTAGAACGCGCGTGGACCAATTGCCGGCCCAAACCAGGCGAGAATATTTCCCGGATTAACAg > 2:196094/1‑139 (MQ=255) aTCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑T‑‑cccccc > 2:220079/1‑71 (MQ=255) aTCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑T‑‑cccccc < 1:220079/71‑1 (MQ=255) aCCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCaaa > 1:873603/1‑139 (MQ=255) ccATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTCCGACCCGGCGGGACCCAATGGCCGCCCTTACCAAGCCAAGATTATTTTCCGGATAGCCAGCaaaa > 2:843560/1‑139 (MQ=255) cATGCTGCATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAAc > 2:938079/1‑139 (MQ=255) cATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAAcgcg < 2:1347728/81‑1 (MQ=255) cATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAAcgcg > 1:1347728/1‑81 (MQ=255) cATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAgg > 1:477193/1‑109 (MQ=255) cATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAgg < 2:477193/109‑1 (MQ=255) tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCgg < 1:1198096/96‑1 (MQ=255) tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCgg > 2:1198096/1‑96 (MQ=255) tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAg > 1:371812/1‑139 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑t < 2:584287/57‑1 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑t > 1:584287/1‑57 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCCCTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAgg > 1:1075758/1‑139 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTaa < 1:16371/101‑1 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTaa > 2:16371/1‑101 (MQ=255) aatgaaAGCTGCACTTGCTTTAGCGTCTAGTGCCATAAACGCCTCCCGAACCAC‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAAAAGGAAAc > 2:827241/1‑139 (MQ=255) gaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc > 1:284762/1‑85 (MQ=255) gaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc < 2:284762/85‑1 (MQ=255) aGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGtctc > 2:182952/1‑139 (MQ=255) gCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAAc < 2:574275/88‑1 (MQ=255) gCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAAc > 1:574275/1‑88 (MQ=255) gCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTcc > 2:88328/1‑139 (MQ=255) tttAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGcc < 1:182952/139‑1 (MQ=255) ttAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCg < 1:87342/122‑1 (MQ=255) ttAGCGTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCg > 2:87342/1‑122 (MQ=255) gTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc > 2:999865/1‑64 (MQ=255) gTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc < 1:999865/64‑1 (MQ=255) gTCTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca < 1:938079/139‑1 (MQ=255) cTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa < 2:395159/127‑1 (MQ=255) cTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa > 1:395159/1‑127 (MQ=255) cTACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGcaca > 2:1288624/1‑139 (MQ=255) tACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc < 2:500406/61‑1 (MQ=255) tACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc > 1:500406/1‑61 (MQ=255) tACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAg > 2:1379177/1‑99 (MQ=255) tACTGCCATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAg < 1:1379177/99‑1 (MQ=255) cATAAACGCCTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACg < 1:88328/139‑1 (MQ=255) cTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc < 2:15319/46‑1 (MQ=255) cTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc > 1:15319/1‑46 (MQ=255) cTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCga < 1:840537/63‑1 (MQ=255) cTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCga > 2:840537/1‑63 (MQ=255) cTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCa > 2:754372/1‑86 (MQ=255) cTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCa < 1:754372/86‑1 (MQ=255) cTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCcagccagc > 1:852491/1‑139 (MQ=255) cTCGCGAACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCcagccagc > 1:1041291/1‑139 (MQ=255) | AAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACC‑T‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGC > NC_000913/2734403‑2734662 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |