| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,101,039 | (C)7→8 | coding (585/720 nt) | yggN ← | DUF2884 family putative periplasmic protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,101,032 | 1 | . | C | 98.0% | 172.9 / ‑3.4 | 50 | coding (592/720 nt) | yggN | DUF2884 family putative periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (22/27); total (23/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01 | |||||||||||
AAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGCG > NC_000913/3100894‑3101165 | aaaTAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAg < 1:1337189/139‑1 (MQ=255) aaTAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑c < 2:726499/139‑1 (MQ=255) gagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑c < 2:1368804/135‑1 (MQ=255) gagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑c > 1:1368804/1‑135 (MQ=255) agGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑accccc < 2:915689/139‑1 (MQ=255) attattTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑cccccccca < 1:1141723/139‑3 (MQ=255) aaaTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTccc < 2:1311316/139‑1 (MQ=255) aaTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCa < 1:1276323/139‑1 (MQ=255) aaTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCa < 2:760231/139‑1 (MQ=255) ttCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGc < 1:1251995/139‑1 (MQ=255) ccGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGcaca > 2:559571/1‑139 (MQ=255) ccGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGcaca > 1:1175723/1‑139 (MQ=255) gACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACAtt < 2:599256/139‑1 (MQ=255) gACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACAtt < 1:486710/139‑1 (MQ=255) ttGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATggg > 1:815497/1‑139 (MQ=255) tCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTAccgccg < 1:1165854/139‑1 (MQ=255) tCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTAccgccg < 2:815497/139‑1 (MQ=255) tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTccc < 1:1057073/102‑1 (MQ=255) tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTccc > 2:1057073/1‑102 (MQ=255) tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGcac > 1:1158345/1‑107 (MQ=255) tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGcac < 2:1158345/107‑1 (MQ=255) tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCa > 2:698224/1‑139 (MQ=255) tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCa > 1:1146877/1‑139 (MQ=255) acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCAc > 1:1163514/1‑138 (MQ=255) acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCAc < 2:1163514/138‑1 (MQ=255) aaCGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccccccccca < 1:383234/91‑4 (MQ=255) aaCGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccccccccca > 2:383234/1‑88 (MQ=255) gCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgc > 1:187845/1‑139 (MQ=255) cTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg < 2:950755/139‑1 (MQ=255) cATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAttt < 2:187845/139‑1 (MQ=255) tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGca < 2:862996/82‑1 (MQ=255) tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGca > 1:862996/1‑82 (MQ=255) ccAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg < 2:447292/139‑1 (MQ=255) ctgctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt < 2:802829/124‑1 (MQ=255) ctgctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:802829/1‑124 (MQ=255) ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACAttacaga < 2:633092/81‑6 (MQ=255) ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACAttacaga > 1:633092/1‑76 (MQ=255) ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTaa < 1:937749/82‑1 (MQ=255) ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTaa > 2:937749/1‑82 (MQ=255) ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg < 2:1114242/131‑1 (MQ=255) ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg > 1:1114242/1‑131 (MQ=255) ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTg > 2:210197/1‑139 (MQ=255) ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGCTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTg > 2:447877/1‑139 (MQ=255) tttCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑c > 1:568386/1‑41 (MQ=255) tttCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑c < 2:568386/41‑1 (MQ=255) ccTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCa < 1:698224/139‑1 (MQ=255) gCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGcaca < 1:826397/57‑1 (MQ=255) gCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑c < 2:1413561/35‑1 (MQ=255) gCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑c > 1:1413561/1‑35 (MQ=255) gCTTTTTCCACTCGGTTTGGATTGAGGATAGCAGC‑CCCCCCCAGG‑CTTCCCAGcaca > 2:826397/1‑57 (MQ=255) cTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGc < 1:447877/139‑1 (MQ=255) cTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGc < 2:1175723/139‑1 (MQ=255) cGGTTTGGATTGAGGATTGCAG‑‑CCCCCCCCGGCCTTCCCAGCCCTTTCTGTAATGGGTTACCGCCGC‑TTTCAGCACCCTTTTCCCGCCCCTTCCATTAATGCGGCCCTGTAAAATTCCCCCCATTGCCTGATTCACTaa > 2:283605/1‑139 (MQ=255) gTTTGGATTGAGGATTGCAGCCCCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTaa > 1:1026073/1‑139 (MQ=255) tttGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTa > 2:1427975/1‑109 (MQ=255) tttGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTa < 1:1427975/109‑1 (MQ=255) tttGGATTGAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAAtt > 1:1219661/1‑139 (MQ=255) gAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAt < 2:1046032/86‑1 (MQ=255) gAGGATTGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAt > 1:1046032/1‑86 (MQ=255) tGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg < 2:174286/85‑1 (MQ=255) tGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg > 1:174286/1‑85 (MQ=255) tGCAGC‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGgcg < 1:210197/139‑1 (MQ=255) | AAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑CCCCCCCAGG‑CTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGCG > NC_000913/3100894‑3101165 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |