| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,358,163 | G→A | A228V (GCG→GTG) | rhmA ← | 2‑keto‑3‑deoxy‑L‑rhamnonate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,358,163 | 0 | G | A | 97.7% | 130.2 / ‑6.4 | 43 | A228V (GCG→GTG) | rhmA | 2‑keto‑3‑deoxy‑L‑rhamnonate aldolase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (26/16); total (26/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GGCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGTAGCCCAACG > NC_000913/2358031‑2358298 | ggCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGc < 2:1183833/139‑1 (MQ=255) cTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGc > 1:47469/1‑139 (MQ=255) ttATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGACATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACACAGTTCGCTCCCCACACCAGGAATTG‑CTGCGCCATATCAGGAGCcac > 1:810343/1‑139 (MQ=255) tGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCCTGCGCCATATCAGGAGCCACAGCCAg > 2:390821/1‑139 (MQ=255) tGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGa > 2:1116717/1‑139 (MQ=255) tGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGa > 1:265541/1‑139 (MQ=255) gCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCt < 2:47469/139‑1 (MQ=255) tttAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCcac > 2:613445/1‑114 (MQ=255) tttAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCcac < 1:613445/114‑1 (MQ=255) cATCGCCAGTCGTTGATCCAGGGCACCGCTGCAGAGCACCGTGTCAACGCCCACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGCGCCACAGCCAGAAAACCAGCCGCTTTACCCgcagca < 2:265541/139‑1 (MQ=255) cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTG‑CTGCGCCATATCAGGAGCcaca < 1:914478/106‑1 (MQ=255) cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCcaca > 2:914478/1‑106 (MQ=255) cAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGAt < 1:390821/139‑1 (MQ=255) gTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATccg < 2:770328/139‑1 (MQ=255) ggCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGtt > 2:1344476/1‑139 (MQ=255) tCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCaa > 1:1122705/1‑139 (MQ=255) cATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGc < 1:315026/69‑1 (MQ=255) cATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGc > 2:315026/1‑69 (MQ=255) cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCcaca > 2:1172505/1‑70 (MQ=255) cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCcaca < 1:1172505/70‑1 (MQ=255) cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAg < 2:1080970/83‑1 (MQ=255) cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAg > 1:1080970/1‑83 (MQ=255) cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATa < 1:714758/115‑1 (MQ=255) cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATa > 2:714758/1‑115 (MQ=255) cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACt > 2:98550/1‑139 (MQ=255) tgtCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTc > 2:1040862/1‑139 (MQ=255) tgtCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTc > 2:818526/1‑139 (MQ=255) tgtCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTc > 2:1197797/1‑139 (MQ=255) tgtCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTc > 1:328055/1‑139 (MQ=255) gCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGc > 2:1325868/1‑139 (MQ=255) gCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGc < 2:492316/139‑1 (MQ=255) aaCAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTAcc > 2:190011/1‑81 (MQ=255) aaCAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTAcc < 1:190011/81‑1 (MQ=255) cAGCGACAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGc < 1:1197797/139‑1 (MQ=255) aCAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCg > 2:578865/1‑76 (MQ=255) aCAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCg < 1:578865/76‑1 (MQ=255) aCAAAGTTCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTAt > 1:847533/1‑139 (MQ=255) ttCGCTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGt > 2:144195/1‑139 (MQ=255) cTCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGGCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGTAGcc > 1:1414183/1‑139 (MQ=255) tCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGcc > 1:1256186/1‑37 (MQ=255) tCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGcc < 2:1256186/37‑1 (MQ=255) tCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCcaca > 1:1363419/1‑40 (MQ=255) tCCCCACACCAGGCATTG‑CTGCGCCATATCAGGAGCcaca < 2:1363419/40‑1 (MQ=255) cacaCCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGTAGCCCAacg < 2:847533/139‑1 (MQ=255) | GGCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTG‑CTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGTAGCCCAACG > NC_000913/2358031‑2358298 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |